EMAGE mouse gene expression spatial database

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EMAGE:21504

Shf Src homology 2 domain containing F ( MGI:3613669)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001116_01	euxassay_001116_02	euxassay_001116_03	euxassay_001116_04	euxassay_001116_05

euxassay_001116_06	euxassay_001116_07	euxassay_001116_08	euxassay_001116_09	euxassay_001116_10

euxassay_001116_11	euxassay_001116_12	euxassay_001116_13	euxassay_001116_14	euxassay_001116_15

euxassay_001116_16	euxassay_001116_17	euxassay_001116_18	euxassay_001116_19	euxassay_001116_20

euxassay_001116_21	euxassay_001116_22	euxassay_001116_23	euxassay_001116_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21504_wholemount_strong.wlz

21504_wholemount_moderate.wlz

21504_wholemount_weak.wlz

21504_wholemount_possible.wlz

21504_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21504_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex

weak

regional

weak expression: see section 3 4 5 6 7 8 9 0 1 2 4 5 6 7 8 9 0 1 2 3

medulla oblongata basal plate

moderate

homogeneous

moderate expression: see section 6 7 weak expression: see section 7 8 9 0

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 2 3 4

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 3 4 5 6 7 8 9 0

facial vii ganglion

weak

homogeneous

weak expression: see section 5 6 7 0 1 2

glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 7 8 9

trigeminal v ganglion

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 9 8 9 0 1 2 3

vagus x ganglion

weak

homogeneous

weak expression: see section 9 0 7 8

vestibulocochlear viii ganglion

weak

homogeneous

weak expression: see section 6 7 8 8 9

dorsal root ganglion

weak

regional

weak expression: see section 8 9 0 1 2 3 6 7 8 9

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4191
Entity Detected:	Shf, Src homology 2 domain containing F ( MGI:3613669)
Sequence:	sense strand is shown >T4191 CGAACGCGTGGGAAAGGATCCAGGATGTAGCATCTGAGCTGTCCCATGGGCTGACCTAGGCTCCTCATTA TTCAGAAATCAATCAACTCCCAACCATCTAGGAACAGAATTTGAGAGATCCCAGAGTATTCCCCGAGGAA AAGCGAAGCCAGACAAGTTGGCAATCCTAGAGGACTATGCGGACCCATTTGATGTTCAGGAGACTGGTGA AGGCTCAGGAGGGACTTCAGGAGCCCCGGGGAAGGTCCCTGAGAATGATGGCTACATGGAGCCCTACGAG GCCCAAAAGATGATGGCTGAGATCCGGGGTTCTAAGGAGACTGCAGCTCAACCCCTACCTCTCTATGACA CACCCTATGAGCCAGAAGATGAGGGGGCCAGCCCAGAAGGTGAGGGGACTCCCTGGCCCAGGGAGTCGCG CCTGCCAGAGGATGACGAGAGGCCTCCAGAAGAGTATGACCAGCCCTGGGAGTGGAAGAAGGAACGGATT TCCAAAGCCTTTGCTGCCCAGTTTGAAGGATCGGAGAAGAACTGCCTGTCACCTGGCCGGGAGGAGAAGG GGCGGCTACCTCCCAGGCTATCTGCTGGGAACCCCAAGTCAGCCAAGCCCCTAGGCATGGAACCCAGCAG CCCCCTGGGGGAGTGGACAGATCCAGCACTGCCTCTGGAAAACCAGGTCTGGTACCACGGGGCCATCAGC CGAACAGATGCCGAGAACCTGCTCAGGCTGTGCAAAGAGGCCAGCTACCTGGTGCGCAACAGTGAGACCA GCAAAAATGACTTCTCTCTCTCCCTCAAGAGCAGTCAGGGCTTCATGCACATGAAGCTGTCCCGGACTAA GGAACACAAGTATGTGTTGGGCCAGAACAGCCCACCCTTCAGCAGCGTCCCTGAAATCGTGCACCACTAT GCCAGCCGCAAGCTGCCCATTAAGGGAGCGGAACACATGTCCCTGCTCTACCCTGTGGCCATCCGGACTC TGTAGATGTGAGACCAGGGCACTATGATAGACCTGGACCCAGTCCTTGTCACCTGCACGGGGGCAGCGGT TCTTACCAAGGACATGATGTCAGAAACCTGGCTTCCCCTTGTCCTAGGATCTAGCAGGCGCTGAAGATTC CTTAATTTATTATAAAGGGGAGGGAATCCTGGGCCTTGGAATAAAGGGACTGTGTGGAGCTGGGCACCGA GGAGAGCCTGAGGAGGAAGGGGTCTCCCCGCCCCAGTCAGACACTAGGGAGGGAGTGGCGACCTCCCTCC CGCACCACCCACCCTCCGCATCCTCCCTTCTCCCTGTCCCCAGCGGGCCGCCCGGGCTCCGGGCCAGAGG CTCGGCCAGGCAGAGCTGCGGCTGCCGGCGCCGCCCAGGGGCTGCCGGGAGGGGGCGGGGAGCGAGCCCG ACTATAAATTGAGGCTGGAGCCGACGCCGGCCGCCTGCGAAAAGCAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4488005 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4488005 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001116 same experiment
	EMAGE:21502 same embryo
	EMAGE:21501 same embryo
	EMAGE:21503 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source