EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:21519

Ccdc9 coiled-coil domain containing 9 ( MGI:1921443)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002945_01	euxassay_002945_02	euxassay_002945_03	euxassay_002945_04	euxassay_002945_05

euxassay_002945_06	euxassay_002945_07	euxassay_002945_08	euxassay_002945_09	euxassay_002945_10

euxassay_002945_11	euxassay_002945_12	euxassay_002945_13	euxassay_002945_14	euxassay_002945_15

euxassay_002945_16	euxassay_002945_17	euxassay_002945_18	euxassay_002945_19	euxassay_002945_20

euxassay_002945_21	euxassay_002945_22	euxassay_002945_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21519_wholemount_strong.wlz

21519_wholemount_moderate.wlz

21519_wholemount_weak.wlz

21519_wholemount_possible.wlz

21519_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21519_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4340
Entity Detected:	Ccdc9, coiled-coil domain containing 9 ( MGI:1921443)
Sequence:	sense strand is shown >T4340 GAAGTACAAGTGCATTTTCCTGTGAGTCTGTGTTTTTGGAACCCTCAATAAAGGAGAAGAGACAGCATTT CAGAATGCTGTTGATCTTAACATACTGTTCTCTCTGCTTGCAGGAGCTTCCAGTGCTACATGTATTTTCT GTGAGGGAGATTACAGCCAATGATGGCTACCACACTAGATTTGAAGTCAAAGGAGGAGAAGGATGCAGAA TTGGACAAGAGGATCGAGGCTCTTCGTCGGAAGAATGAGGCCCTCATCCGGCGCTACCAGGAGATTGAGG AGGATCGGAAAAAAGCTGAACTGGAGGGCGTGGCAGTGACAGCCCCCCGGAAGAGCCGCTCCATGGAGAA GGAGAATATGGCAGTGGAGGAGAAGAGCCTGGGTCCCTCTCGGAGGACTCCTGGGACTCCTCGACCCCCA GGGGCCAGCAGAGGGGGCCGGACCCATCCCCAGCAGGGAGGCCGGGCAGGCGTAGGCCGGGCATCCCAAG GCTGGGAGGATGGTGCTGGGGAGCAGCTTCGAGGGGGTCCTGGGGGCCGGGGCCGGAGGGGCCGGGGCCG GGGATCCCCTCATCTCTTAGGGGCAGGAGACAACTCAACCTCTGATCGAAAGTCTAAGGAGTGGGAGGAG CGGCGCAGGCAGAATATCGAGAAGATGAATGAGGAGATGGAGAAGATTGCCGAATATGAGCGCAACCAGC GGGAAGGGGTGCTGGAGCCCAACCCAGTACGCAACTTCCTGGATGATCCCCGACGTCGGGGTGGGCCCCT CGAGGAGTCTGAGAGGGATCGCCGGGAAGGCAGCCGCCGGCATGGACGCAACTGGGGAGGCTCAGATTTT GAGCGGGTGCGCTCTGGCTTGGAACAGGAGCGACAGGGCCGCAGGGCTGGCCTGGGCAGTGGGGGCGACA TGACGATGTCCATGACGGGCCGGGAGAGGTCAGAGTACCTGCGCTGGAAGCAGGAGAGGGAGAAGATCGA CCAGGAACGTCTGCAGCGACACCGCAAGCCCACCGGGCAGTGGCGGCGGGAGTGGGATGCCGAGAAGACC GATGGCATGTTCAAGGATGGCCCAGCCCCTACCCATGAACTGTCCCACCGCTATGATGACCAAGCTTGGG CAAGACCACCTAAGCCTCCCACTTTTGGAGAGTTCCTTTCTCAGCACAAAGCTGAAGTCAGCAGCCGCAG AAGGAGAAAGAACAGCCGACCTCAGGCCAAGGTGGCCCCTCGTGCCTATAGTGACCACGATAATCGCTGG GAGACAAGAGAGGAAGCTGTGTCCTCGGCCCCCGAGTCCTCTCAGTCCATATCCCTGGAGGAGACACCCA CACAGGCTTCTGAGACCCCAGCTCCAGCTCATCGGCCTCCCGAGGAAGATGGTGAGGAAGATGTGGGGGA AGAGGAGGAGGGAGAAGAAGAGGGGGAAGATGAGGAGGATGAAGAGTGGGAGGACGTGAGTGAAGATGTT ACTGAGGAGGAGGAGGAAGAGGAAGAAGAGTTTGAAGAGGATGAAGAGGGTCCTAAGGACCAGGAAGCAG CAACTGTTCCTGATCACCAGCCTGAAGCTGAGCCTGCTGGGAAGCCCACCTGTGAGCAGGTGGACCCTGT GCCTGCCGGGTCCCAGGAGTTGCTGTCCCCAGTCCCTGTTGAACCTCCCATCCCCTTCTCACCCTCTGAG GACCACCAGCCTGTGTCTGACTGGGGTGAGGAGATGGAACTGAATTCTCCCGGCACTGCCCACTTACCTG GTACCCACTCTTCGGGTGAGGCCTGGCCTTTTGCGAATGCTTGAAACTGGCTGCTTGTGTGTGTGTTTGT GTGCACAAGGGTGTGGCTGTGCCCACACCTCCTTCAACCCCCGTGGCAGAGAAGGGCAGTTGGTGCCTGC TTCCTGCCCTTATCTCAGTTCCCAAAGCCTGCTCTTGCTGGTTCAGAGCCTCTCTCCAATAAAAAGTTCA CTTCCTTAAATGACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:4527178 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527178 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002945 same experiment
	EMAGE:21521 same embryo
	EMAGE:21523 same embryo
	EMAGE:21520 same embryo
	EMAGE:21522 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source