EMAGE mouse gene expression spatial database

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EMAGE:21520

Ppp4r1 protein phosphatase 4, regulatory subunit 1 ( MGI:1917601)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002915_01	euxassay_002915_02	euxassay_002915_03	euxassay_002915_04	euxassay_002915_05

euxassay_002915_06	euxassay_002915_07	euxassay_002915_08	euxassay_002915_09	euxassay_002915_10

euxassay_002915_11	euxassay_002915_12	euxassay_002915_13	euxassay_002915_14	euxassay_002915_15

euxassay_002915_16	euxassay_002915_17	euxassay_002915_18	euxassay_002915_19	euxassay_002915_20

euxassay_002915_21	euxassay_002915_22	euxassay_002915_23	euxassay_002915_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21520_wholemount_strong.wlz

21520_wholemount_moderate.wlz

21520_wholemount_weak.wlz

21520_wholemount_possible.wlz

21520_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21520_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4274
Entity Detected:	Ppp4r1, protein phosphatase 4, regulatory subunit 1 ( MGI:1917601)
Sequence:	sense strand is shown >T4274 CACCATTACACAGACACATATGCAGCATACTAATTGATTTACTTTTGCGGAAAAGGAAGAAACGGCTCCA TTATACTGTCAGGGCCAGTGCTAAAAATAACTTGAAGGATGAGAGACAATGATGTCGTAGAAGAAGAGCA TCGCAGGCCAGAGGACGCTCCTTCAGACCTCAGTGCCCCTCACTCCAGCGCCAGGCTGGAGAGCCTCGAA GGCTGTGCTGCCAAGACACCTGGGCACTCTGCAGGTGACGTGCCTGCTCCAGTGGACAGCTCCTTACTCT GTACTTTGTCCTCAGAGTCTCCTCAGGAAGCAGCTAGCAATGATGAGAATGGTAGAAAGCCTGACACCAA CAGCAAGTCTGCGTCCCGGCCAGACGCTGGCACCAGCTCCCCAGAGGCCACTCCCTTAGACCAGGACATG TTCAACTCCTTCCATTTCTGGAGGACTCCTCTACCTAAAATAGATCTTGATAAAGAGCTCCAGCAGGACC CCGAGGAGAGACTCAGCCCAGAGAGAACAGGAGATGTACCTGCAGCCCCTCTACCAGGCCCTCCCAATAT CACCATGGCTACCCGGAAGGAACTAGAAGAAATGATAGAAAACCTTGAGCCACACATGGATGACCCGGAT GTGAAAGCACAGGTGGACGTGCTGTCGGCTGCCCTGCGAGCTTCCAGCCTGGATGCTCACGAAGAGACTG GTGGTGTAGAGCAGCGGAGTGAGCTACAAGATGAAGTGTGTGTCAGTGAGCTTCCAGACTGTAACATAAG TCACGACACTTGTGTGCCTTTGATCAGCGCTGCTGAGGAGAATGCGGAGGCCACTCCTGATTATGTCCAT GGAGGTGCAGACGTAAGCCCTGGTGATGGCTTCAGCCCGGATGAAGATAGGAGACCCAAAGTCCAGGATG TTGTACCACAAGCGTTACTGGATCAGTACTTGTCAATGACCGACCCTTCTCGAGCACAGACAGTTGACAC TGAGATCGCTAAGCACTGTGCATACAGTCTGCCGGGTGTGGCCCTGACTCTTGGCAGACAGAACTGGCAC TGCTTGAGGGAGACTTACGAGACCCTAGCGTCGGACATGCAGTGGAAAGTTCGAAGGACTCTAGCCTTCT CCATCCATGAGCTTGCAGTGATCCTTGGGGACCAGCTGACAGCTGCAGACCTGGTTCCAATCTTCAATGG ATTTTTAAAAGACCTCGATGAAGTCAGGATAGGTGTTCTTAAACACTTGCATGACTTTCTGAAGCTTCTT CACATTGATAAAAGAAGAGAGTACCTTTATCAGCTCCAGGAGTTTTTGGTGACAGACAACAGTAGAAATT GGCGCTTTCGAGCTGAACTGGCAGAACAGCTGATTTTACTTCTGGAGTTATACAGTCCCAGAGATGTTTA TGATTACCTACGACCCATCGCTCTGAATCTGTGTGCAGACAAAGTTTCTTCAGTCCGCTGGATTTCCTAC AAGTTGGTCAGTGAAATGGTGAAGAAACTCCACACAGCAACACCACCAACCTTCGGAGTAGATCTCATCA ATGAGCTGGTAGAGAATTTTGGCAGGTGTCCAAAATGGTCTGGTCGGCAAGCCTTTGTCTTTGTCTGCCA GACTGTCATTGAAGACGACTGCCTCCCCATGGACCAGTTTGCTGTGCACTTGATGCCACATTTGCTGACC TTGGCAAATGACAGGGTTCCCAATGTTAGAGTGCTGCTTGCGAAAACCCTGCGACAGACTCTGCTGGAGA AAGAGTACTTCTTAGCCTCTGCCAGCTGTCACCAGGAGGCCGTGGAGCAGACAATCATGGCCCTGCAGAT GGACCGAGACAGTGATGTCAAGTACTTTGCAAGCATTCACCCCTCCAGTACCAAACTCTCTGAAGATGCA ATGAGCACAGCTTCCTCCACCTACTGACCCACGGTGTCCATCCTGCATCCGCAAGAGCCTGGCCTCGACT GCCTGCACCACTCGGGACAGCTGTGGTGGGGGCCTTCCTCCTGCCAACTCATTCGCAGGTGCAGGTTGCC TACTCCCAATACCAGTGGTTTTAAGAGTCAAGAGAAAGTACAGTAAACACTATTATCTTATCTTGACTTA AGGGAAAGTAAATCTCAGAGGATTATAATTGTCACCAAAGCCTTAACTCGTTCCTTCATCTTCCTGACTG ATGGCTTGAATTGGCAGAGCATTTTCCCTTCGGGAAGGATGAGGTTCCCAGAGACCTGCGTTGCTTTCTT CTGGTTTTATTTAACGGCTGATAAATGACATTCTTACGGCCAGAAGGTGGATGTGCACCGGACAGGAAAG GCCTTCAGTATTAGCCTAAGCATTTCTTCTAGGTGCTGAGCCTGTTCAGCTCCTTGGCTGCCCTGCCCAG CCCAGCCCTAAGTGTGAATAGTTCTTGGCGTGTATAAATGACAAAGGAGTTTTTCCTCTCCTAAGGGCTT GATGTTAACACTAAATAGAATCTGATTTTGACTCCTAATGCAGCACATTGCTGTACACATTTACAGAATG TTTGCAGAATCTCTCCTGATATTTTTCATGCTGGTCATGACCTGAAGGAAATTTCTTAGCACGAATATTG TGTCAGGTATTTTTAACTTGATCATGGTCAGCTCTGAGGTGCAACTGTGTCTTCCCGTGCTGCACCCCTG TGACCACTCTGGGGTGTGCAGCCTGAATCATGCTGTTAGAACTGTTGTGGTACAAGCTCCAAATAAAATT TATTAATAGGATCTATAAAGAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504305 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504305 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002915 same experiment
	EMAGE:21521 same embryo
	EMAGE:21523 same embryo
	EMAGE:21522 same embryo
	EMAGE:21519 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source