EMAGE mouse gene expression spatial database

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EMAGE:21569

Nop14 NOP14 nucleolar protein homolog (yeast) ( MGI:1922666)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001210. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001210_01	euxassay_001210_02	euxassay_001210_03	euxassay_001210_04

euxassay_001210_05	euxassay_001210_06	euxassay_001210_07	euxassay_001210_08	euxassay_001210_09

euxassay_001210_10	euxassay_001210_11	euxassay_001210_12	euxassay_001210_13	euxassay_001210_14

euxassay_001210_15	euxassay_001210_16	euxassay_001210_17	euxassay_001210_18	euxassay_001210_19

euxassay_001210_20	euxassay_001210_21	euxassay_001210_22	euxassay_001210_23	euxassay_001210_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21569_wholemount_strong.wlz

21569_wholemount_moderate.wlz

21569_wholemount_weak.wlz

21569_wholemount_possible.wlz

21569_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21569_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 1 weak expression: see section 8 9 0 8 9 0

foregut-midgut junction

weak

regional

weak expression: see section 3 4 5 6 7 8 9

facial vii ganglion

weak

regional

weak expression: see section 5 6 7 8 1 2 3

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 7 8 9 0

trigeminal v ganglion

weak

regional

weak expression: see section 4 5 6 7 8 9 0 9 0 1 2 3 4

vagus x ganglion

weak

regional

weak expression: see section 9 8

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 6 7 8 9 8 9 0

dorsal root ganglion

weak

regional

weak expression: see section 7 8 9 0 1 2 3 4 7 8 9 0

pharyngo-tympanic tube

weak

regional

weak expression: see section 1 2 3 4 5 4

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 0 1 2 3 4 5 6 7 8 9 0 1

anal canal

weak

regional

weak expression: see section 6 7

esophagus

weak

regional

weak expression: see section 4 5

stomach

weak

regional

weak expression: see section 4 5 6 7 8 9 0 1

hindgut

weak

regional

weak expression: see section 5 6 7

midgut

weak

regional

weak expression: see section 9 0 1 2 3 4 5 6 7 8 9 0

bladder

weak

regional

weak expression: see section 5 6

urethra of male

weak

regional

weak expression: see section 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4660
Entity Detected:	Nop14, NOP14 nucleolar protein homolog (yeast) ( MGI:1922666)
Sequence:	sense strand is shown >T4660 CCAAAGTGTGGCGGAGTGAATGTCATGGGGAAAGCCAAGCGGACTGGGGCGCGGAGGCAGGTCCACAAAG CGCCCACAGGGGCGTCCGGAGGCCCGGCGAAAACTAACCCAAACCCATTTGAAGTGAAAGTCAACCGGCA GAAGTTCCAGATCCTTGGCAGGAAGACGCGCCACGATGTGGGCCTGCCCGGGGTGTCCCGCGCTCGTGCC ATCAGGAAGAGAACCCAAACTTTACTAAAGGAGTACAAAGAAAGGAACAAGTCCAACGTGTTCGCAGACA AACGCTTTGGGGAGTATAATAGCAACATAAGCCCAGAGGAAAAGATGATGAAGAGATTTGCTCTGGAGCA ACAGCGATATCATGAGAAAAAGAACATCTACAACCTAAATGAAGATGAAGAATTGACTCATTATGGCCAG TCCTTGGCTGACATTGAAAAGCATAATGACATTGTGGACAGTGACAGTGATACTGAAGACCGGGGGGCAT TGTCAGCTGAACTGACTGCTTCCCACTTTGGTGGTGGGGTCCACAAGAACAGCTCTCAGAAGGAGGGCGA GGATGGAGACAAGCCAAAGACACGGAAGGAACTGATTGAGGAGCTCATTGCAAAGTCAAAGCAAGAGAAG AGGGAAAGACAGGCTCAGCGAGAAGATGCCCTTGAGCTCACAGAGAAGCTGGACCAAGACTGGAAAGAAA TACAGATTCTCATGTCACGAAAACCTAAGAAGTCAGAAAACAAAGAGAAAAAGGAAAAACCCCAGCCCGA TGAATATGACATGATGGTTCGTGAGCTTGGCTTTGAAATGAAGGCTCAGCCCTCTAACAGGATGAAAACA GAAGAGGAACTAGCAAAGGAGGAGCAAGAGAGGCTCAAGAAGCTGGAGGCTGAAAGACTTCGAAGAATGC TTGGAAAAGATGAACATGAAAATAAAAAGAAACCAAAACATACATCAGCAGATGATTTAAATGATGGCTT TATACTGGATAAAGACGATAGGCGCTTGCTTTCTTACAAGGATGGGAAGATGAACATTGAAGATGTCCAG GAAGAGCAAAGCAAGGAAGCAGATGGCCAAGAGAATGACCAGAAAGAGGGAGAAGATGAGAGTGAGGAAG AGGATGAGAGCCATGAGGACTCAGAAGAGAGTGAGGACCCAGACAGCCACTCAGACCTAGAATCTAATAT AGAGAGTGAGGAGGAAAACGAGACACCAAAGAAGGAGCAACGTCAGACTCCTGGGGGGAAGTTGCCAAAG GATGACCAGAAAGCTCAAAAGGCTGTTGCAGCTGAGCTGCCCTATGTCTTTGCAGCTCCTGAATCCTTTG AGGAGCTGAAGTTTTTATTGTCAGGAAGATCAATGGAAGAGCAACTTTTGGTGGTGGAAAGAATTCAGAA GTGCAACCATCCAAGTCTTGCAGTAGGAAACAAAGCAAAATTGGAAAAATTGTTTGGCTTCCTCTTGCAA TACATAGGAGATTTGGCTATAGACAGCACACCGGACCTCAAGACCATCGATAAGTTGGTTATGCACTTAT ATAGTCTTTGCCAGATGTTTCCTGAGTCTGCAAGTGACTCCTTAAGATTTGTTCTCCGAGATGCCATGCA CGAGATGGAAGAAATGATTGAGACCAAAGGCCGGGCGGCGTTTCCAGGGTTGGATGTGCTCATTTATTTG AAAATCACTGGGCTGTTGTTTCCAACCTCTGATTTCTGGCATCCAGTTGTGACTCCTGCCCTGCTGTGTA TGAGCCAGATGCTTACTAAGTGTCCAGTCATGTCCCTACAAGATGTGATAAAGGGCCTGTTTGTGTGCTG CTTATTCCTGGAGTACGTGTCCCTGTCTCGGAGGTTCATCCCCGAGCTATTCAATTTTCTACTTGGGATT CTTTATATAGCAACTCCAAATACAAAGAGCCAAGGTTCCACTCTGGTGCACCCTTTTAGAGCACTTGGGA AGAACTCAGAATTGCTCGTGGTCTCCGACAAAGCAGATGTGGCCACCTGGCAGAGGGGAAGCCTCCCCTT GCACTGGGCAAATAGACTGAGCACCCTGACAGCGACTGAGGCAAATCACACCAGGCTGTCCTGCGTAGCC TTGTGTTTGAGCCTGATGAAGCACTGCGTGCTCATGTATCAGGCACTGCCGTCCTTCCACGCCATCGTCA GGCCCCATCAAGCCCTCCTGGCGAAGCATCTTGCAGATTGTAGCCTCCCCCAAGAACTTAAGGAGCTGGC TCAAAGCATACTGTCAGCAATGGAAGGCCTGAAGCAGCACTGCCGGCCGCTGGTGTGTGAGAAGAGCAAG CCCGTCCCACTGAAGCAGTTCACCCCCCGGCTAGTCAAAGTGCTTGAGTTTGGACGGAAACAAGGCAGCA GTAAAGAAGAACAGGAAAGGAAAAGACTAATCCACAAACACAAGCGGGAATTTAAAGGCGCCGTCCGAGA GATCCGAAAGGACAATCAGTTCCTGGCCAGGATGCAGCTCTCAGAAATCATGGAAAGGGATGCAGAAAGA AAGCGTAAAGTAAAACAGCTTTTTAATAGCCTGGCCACGCAAGAAGGTGAATGGAAGGCTCTTAAGAGGA AAAAGTTCAAAAAATAAATTGTCTTGTAATGTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5012070 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5012070 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001210 same experiment
	EMAGE:21573 same embryo
	EMAGE:21572 same embryo
	EMAGE:21571 same embryo
	EMAGE:21570 same embryo
	EMAGE:21574 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source