EMAGE mouse gene expression spatial database

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EMAGE:21572

C030048B08Rik RIKEN cDNA C030048B08 gene ( MGI:2442653)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001233_01	euxassay_001233_02	euxassay_001233_03	euxassay_001233_04	euxassay_001233_05

euxassay_001233_06	euxassay_001233_07	euxassay_001233_08	euxassay_001233_09	euxassay_001233_10

euxassay_001233_11	euxassay_001233_12	euxassay_001233_13	euxassay_001233_14	euxassay_001233_15

euxassay_001233_16	euxassay_001233_17	euxassay_001233_18	euxassay_001233_19	euxassay_001233_20

euxassay_001233_21	euxassay_001233_22	euxassay_001233_23	euxassay_001233_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:21572

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21572_wholemount_strong.wlz

21572_wholemount_moderate.wlz

21572_wholemount_weak.wlz

21572_wholemount_possible.wlz

21572_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21572_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

two stars

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 3 4 5 6 7 8 9 0 9 0 1 2 3 4

c2 nucleus pulposus

moderate

regional

moderate expression: see section 3

c3 nucleus pulposus

moderate

regional

moderate expression: see section 3

c4 nucleus pulposus

moderate

regional

moderate expression: see section 3

c5 nucleus pulposus

moderate

regional

moderate expression: see section 3

c6 nucleus pulposus

moderate

regional

moderate expression: see section 3

submandibular gland primordium

weak

regional

weak expression: see section 7 8 9 0 1 7 8 9 0

foregut-midgut junction

weak

regional

weak expression: see section 3 4 5 6 7

cerebral cortex marginal layer

weak

homogeneous

weak expression: see section 3 4 5 6 7 8 9 0 1 2 3 7 8 9 0 1 2 3 4

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 1 2 3 4

facial vii ganglion

weak

regional

weak expression: see section 4 5 6 0 1 2 3

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 7 8 9

trigeminal v ganglion

weak

regional

weak expression: see section 4 5 6 7 8 9 0 8 9 0 1 2 3 4

vagus x ganglion

weak

regional

weak expression: see section 9 8

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 7 9 0

dorsal root ganglion

weak

regional

weak expression: see section 9 0 1 2 7 8 9

stomach fundus

weak

regional

weak expression: see section 3 4 5 6 7 8 9 0

hindgut

weak

regional

weak expression: see section 4 5

midgut

weak

regional

weak expression: see section 9 0 1 2 3 4 5 6 7 8 9

chondrocranium

weak

regional

weak expression: see section 1 2 3 4 5 6 7 8 9 9 0 1 2 3 4

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4664
Entity Detected:	C030048B08Rik, RIKEN cDNA C030048B08 gene ( MGI:2442653)
Sequence:	sense strand is shown >T4664 CGGACGCGTGGGCTGCTCCACATTGTCCCAAGGTTCAACTCTCACCGGAGAGCGGAGTCCACCGGCTTCT CGTCAGGTGAGAGCGTCGGAACCCGAGAACATCGATCAGGCCCGCCCCCCTCTTCCCACGCCCGGCCGCT GTCACCCTCGGGCCACCTCCGAAGCTCTAGGGGGCGGCACGTCGCTCGTCGAATTCTCGTTCTCAGGAGA TGATCGTCGATGGGGTCGATCCTTCAGCCAATCCTAGCACGCCTGGTCCATCCTGCTCTCTTATTGGACT CAGAGGAGCAGGGCTGGCGGATGTTGTTCTGGCGCGGGGGACTAGAGCCGGCAAGATGGCATCCAGTGAC GGGAAACCTGGTGGCGTATTCGATCACCACGTCCAGACGGCTGTGTGTGACTCGCGGGCCAAATACCGGG AGGGCCGACGCCCTCGCGCGGTCAAGGTATATACAATCAATTTGGAGTCTCAGTACTTACTAATACAAGG AGTTCCTGCAGTGGGAGCCATGAAGGAATTAGTTGAGCGTTTTGCTCTGTATGGTGCGATTGAACAGTAT AACGCTCTAGATGAATACCCAGCGGAAGATTTTACAGAAGTTTATCTCATTAAATTTGTGAAATTACAGA GTGCAAGGGTAGCTAAGAAAAAGATGGATGAACAGAGCTTCTTTGGAGGGTTACTTCATGTGTGCTACGC TCCAGAATTTGAGACAGTTGAAGAAACTAGAAAAAAATTACAAGAAAGAAAGGCTTATATTACAAGAGTT ACTAAAAACCAAGATTGTTACATGGCGAAGAAGAAACCAGTTCCAGAGCAGAAAGGAACAAAAGATTCTA GACAAGGTTTCCATCCACCTATGCCTGGATTTGGTACAGCTGCTTTAAACACATCTCCTGAGAGTCCTCC AGAGAACTCGAGTTCTTGTCTTCCTTATTCTTGTGAATTCCCTTTAAGCTGTTTTGCTTCCAAGAGCACA TGTTCACGTGGGGAACATGTGGACAGAGTATCAGACTCCTGTAATAGTGCTAGGAACCATGGTGAACTGT CTAAGCATCGTGACCACAGTGCCTTTTCACCAAAGCTGCAGATGAACACTTACAAAAACTCCGTCCCTTG CTCTAGTGTGCAAGAGGCCATTGCTACCTCACAGGCAGTTGGCAGGTTCATGCCTAGGACAACACAGCTG CAGGAGAGGAAACGGCGGCGAGACTGTGATCGAGAACTGGGAACTCTGCTTGAGACACACACAAGCAGTA ACGAGGTTTTGATTGGACCTAAGTTACCAGGTATCCCCACAGTAGATCTGCAGGACGACTCCTTGAATAC AACGGCAACCTTAATTCGGAGGAAACTTAAAGAGGTGATTTCATCTGTGCCAAAGCCTCCAGAGGACAAT ATAAAAGACGTGTGTACAAGTCACCCAGGAAAACAAAGAAGAAGAATATAGCTTGCCAGTGGGGAGTTAG TCTTGCCTTTTCTTTTTTCCTTTGAATCAGAGTCTCACTGGATAGCTCTGGCTACTCTGGGACTCTCTAT GTAGACCAGGCTGGCCTGAGTGCTGGGACTAAAGGGTGTGTGCCACTACCCCCAGCTAAATTGGTGTATT CTGGAGAGATTCTTTTTTATGTTTGGCCCATTTTAATTCTTTCAGGCATCTTACTACTGCTGTTTTAAAG TTTTTTTCTTTTAAACCAGTTGCAAATGTTTCCATCTTTCTTGGATCTACATGCAATGTGGCAGAGACTG TTCTCTTTATACTGTATTAGTTCTTATATTAGAATACGTAAATTGATTGAAACAAATGTAGAAATATGTC ATACAAATAAGGCAATCAGGGTCTCATTTTAATTCAGGTATTGAGCAATCTGTGTTCAGAGACTGGCAGG GGGGTTGTTTTTGAACACCAGGGCTTCAAAGTGAAAGCAGAACTGTAGAGAAAAAAATGCCAACAAATGT TACAGCAACATTGATATTAGAAACACATTTTAAAAAAAGCAGCATACATAATGTAACTTCTAAGACTAAT TTAAATTGACTATGGTGATGGAAAAAACTAATTTTGGAAATTCTAGATTTCTACACTGTCAGCATATCTG TACTTTTTCTTCTGTCAGCGGGCCACAGTATAATTTTTCAGGCTATTTATATTTCTTTTCTTGGAAAAAA TAAAGTGAGAAGGCACATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5012510 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5012510 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001233 same experiment
	EMAGE:21569 same embryo
	EMAGE:21573 same embryo
	EMAGE:21571 same embryo
	EMAGE:21570 same embryo
	EMAGE:21574 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source