EMAGE mouse gene expression spatial database

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EMAGE:21607

Acbd5 acyl-Coenzyme A binding domain containing 5 ( MGI:1921409)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004319. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004319_01	euxassay_004319_02	euxassay_004319_03	euxassay_004319_04

euxassay_004319_05	euxassay_004319_06	euxassay_004319_07	euxassay_004319_08	euxassay_004319_09

euxassay_004319_10	euxassay_004319_11	euxassay_004319_12	euxassay_004319_13	euxassay_004319_14

euxassay_004319_15	euxassay_004319_16	euxassay_004319_17	euxassay_004319_18	euxassay_004319_19

euxassay_004319_20	euxassay_004319_21	euxassay_004319_22	euxassay_004319_23	euxassay_004319_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

21607_wholemount_strong.wlz

21607_wholemount_moderate.wlz

21607_wholemount_weak.wlz

21607_wholemount_possible.wlz

21607_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:21607_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 weak expression: see section 1 2 3 4

facial vii ganglion

moderate

regional

moderate expression: see section 3

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 6 7 7 8

trigeminal v ganglion

moderate

regional

moderate expression: see section 2 3 4 5 6 7 8 6 7 8 9 0 1 2

vagus x ganglion

moderate

regional

moderate expression: see section 7 8 7

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 6 7 7 8

spinal cord

moderate

homogeneous

moderate expression: see section 8 9 0 1 2 3 4 5

dorsal root ganglion

moderate

regional

moderate expression: see section 7 8 9 0 5 6 7

not examined

regional

not examined expression: see section 1 2 2 3 4

neural retina

weak

regional

weak expression: see section 1 2 2 3 4

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 9 0 1 4 5 6 7

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5036
Entity Detected:	Acbd5, acyl-Coenzyme A binding domain containing 5 ( MGI:1921409)
Sequence:	sense strand is shown >T5036 GAGATGCTCTTCCTCGCGTTTCATGCAGGCTCTTGGGGAAGCTGGTGCTGCTGCTGCTGTGTGATTACCG CGGACAGACCTTGGGACCGAGGCCGGCGCTGGCAGCTGGAGATGGCGGACACACCGTCAGTGTATGAAAC CAGATTTGAGGCGGCCGTGAAGGTGATCCAGAGTTTGCCGAAAAATGGATCATTCCAACCAACAAATGAA ATGATGCTTAAGTTCTATAGCTTCTACAAACAGGCAACTGAAGGACCCTGTAAACTTTCACGGCCTGGAT TCTGGGATCCTATTGGAAGATATAAATGGGATGCTTGGAGTTCACTGGGTGATATGACTAAAGAAGAAGC CATGATTGCATATGTTGAAGAAATGAAAAAGATTATTGAAACCATGCCCATGACTGAGAAAGTGGAAGAG TTGCTGCATGTTATAGGTCCATTTTACGAGATTGTAGAAGACAAAAAGAGCAGCAAGAGTTCTGACTTGA CCTCAGATCTTGGTAATGTTCTGACCTCTTCAAATGCCAAAGCTGTTAATGGTAAAGCAGAGAGCAGTGA TAGTGGGGCTGAGTCTGAAGAAGAAGAGGCCCAAGAAGAGTTAAAAGGAGCAGAACAGAGCGGCAGTGAT GATAAGAAAACGCTGAAGAAATCAGCAGATAAGAATTTGGAAATCATTGTTACCAATGGCTATAAAGGTA GCTTTGTTCAGGATATACAGAGTGACATTCATACTGATTCTTCCCGGAGCACCAGAAGCAGTGAAGATGA AAAGCCTGGAGATGAGAGCTCTCAGCAGACAGGACACACCATTGTCTGTGCTCACCAAGATAGAAATGAA GATCCCAGTGAAGATGCTTCAGGAATACACCATTTAACAAGTGATTCAGATAGTGAAGTGTACTGTGACT CTATGGAGCAATTTGGACAAGAAGAGTATTATTTAGGTGGTGATCCTACTCAGCACTTGGAAAGTTCTGG TTTTTGTGAAGATGCTCAGCAATCTCCTGGAAATGGCAGCATTGGGAAAATGTGGATGGTAGCAGTCAAA GGAAAAGGCGAAGTGAAACACGGAGGAGAAGATGGCAGAAGTAGCAGTGGAGCACCGCACCGTGAGACGA GAGGTGGAGAGAGCGAGGACTTCTCCAGTGTCAGGAGAGGGAGAGGGAATAGAATACCACATTTGAGCGA AGGACCCAAGGGTCGGCAAGTAGGAAGTGGAGGTGATGGAGAACGCTGGGGCTCAGACCGGGGGTCCAGA GGCAGCCTCAATGAGCAGATCGCTCTTGTGCTCATTAGATTGCAGGAGGACATGCAGAATGTCCTTCAGA GACTTCATAAACTGGAAACACTAACTGCTTCGCAGGCGAAATTATCTTTGCAGACAAGTAATCAGCCCTC CTCACAGAGACCAGCTTGGTGGCCCTTTGAGATGTCCCCTGGTGCATTAGCTTTTGCTATTATATGGCCT TTTATTGCTCAGTGGTTGGCACATTTGTATTATCAAAGAAGGAGAAGAAAACTAAATTGAAGAAATGGCT TTTTACTCAAGAAGACTGCTGGGCCTGGACGACCTAAGAGACGGACTGGAAATGAAGTGGAATTCAGAAG AAAATCTCAGTTTTGATGATTACGTTGCTTTTTTGTCCAGTAGTTTGGTTTATATACATAAATATTCTTT GCACTACACAAATGGTAACATTTTAAAGACTAATATTGCTGATACTTGAAAAATCAATCTCAACTAGATT TTAAGTAGCATCCATAGACTTACTCTTTTTGAGCTTGAAGGACTAAATTATTAATTATTGTTCAGTAATG ATCACCTAATTTTTCCCATAAGAATTATAAGCTGCATTTCTAAGGCATAGCTCCAATTATGAGATAAAAT TTATACTTTTTACTTTTCTAAATTAAACACAAGAAAGAATGCAAAAACAAAAGTGATTTTTTAAAAATGA GGTTAAATCGATTTTGCATCAGTCACTGAACTGTCAGCTACGTGGCAGGAACCGGACGGAAGAATCCTCT CCAGGAGACTGGGCTTTAGTTAGTGGCTTGATTCCCCGGCTTGCTCAAGGGAACTCAGAAAATCATCGTC CTTGTACTGCGCTTCTGTTTACAAACGATTCACTGAACAGACTTAACTTGATGATGGAAATGATGAAAAA CAGGCCTAATGTAGGTGTAAAAGTGTATTCAAACTCTAGACACGGCTAATCAGATTTTAATTCAATCATA CACACATAATCCTTTGTAATCATTTTTCTTTTAAGTGAGGATATCTACTGTCTGCTTCATAGGGTGCTTT GCAATAAAATGAAAACGTATTTATACTGTTTTTACTAAGGTAACAAAAGCATGATAATCCTTTTTTTGCA AGTGTAAACGACAGTCTGGACTCTTAACTTCTTAGTGCCTCAGTTTTCCTTTGGGGTATACTTCACAAGG TATCAGAAGTACTTTCAGTCTACAGGTCTCTATGTCCACATAACTAAGGAATGCCTAACTAATGGAAACT TAAGTTCCTTCTAGCCATTAAAACAGAGAGAAGATCAATTTAGATCCCGGAACACTGTATTTGAAAAATA ATCTTCCTGTGTACTTTCTCAAACCTGCGCTCAGTAATATACTTTATTCTTTGAAGATTTAATTTAAAAA CTTCATAGAAAATCCTTTTTTGCTTTTCCTTTTGAGACAGAGTTTTGGTAGCTCCTACCAGCCTCCAATT CTCTATGTAGCTGAAGATGACCTTGACTACCTGAGTTTCCTGTCTCTACTTCTTTTCAGCTTCTGGGACC ACAGGCCTGTACCACCATGGCCAGCTTTAGAGAGTAAAACATTTTAATTTGTTCTTGATTTGTATGATGT TGGGGGCGTTAGCATTACGTGTTGAATATTTTGAGTAAGAAGAAAAGAATCCATCTTCAGAATATAGGGT TGTTCTTTGGGAAAAAGAGTATAAGAACATAATTTCTTTGTCCTAGACTTTACAAATTTTTCAAAACTGT AACTTTATTTTAAACTTGTGTATCACAACTTGGTTGATATGCCTAAAATGATCTACTGGCTTTTGCTTAT GATTAAAAAACCTCAAATCTATCTTCTACATTTTAAAAGGAAGAAAAAAAAGCAAATAACTGAATTATTA TAGAAAACTTTAGACCTGTGACATTTTTTTGTTTTAAATTACAAAAGAGGTTGGTATGATAAAGTGATGG CTTTTTTCTTATATAAATAGAATGCAAATCAAAGATATAACCAGTGTGATTGATTGCAAGTAGTCAAGTG TAACTAACTCCTAAGGGCAACCATTTGTATCTTATATATCAGAATTACTTTATAGTTTGAAAGCACTTTG CACACATTTCCTGTATATAAAGGATGTAATTACAGGATATAGTGGTAATGCATTAAAACATGAACCTCAT TTTAGAAATCGACAGCTATGGTATTTTTTTAATGATCAGCTTTTCTGTTTATTGTAAAACTAAGTTAAAA AATAAAAGGTTAATGAGAAATGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5257773 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5257773 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004319 same experiment
	EMAGE:21611 same embryo
	EMAGE:21610 same embryo
	EMAGE:21612 same embryo
	EMAGE:21609 same embryo
	EMAGE:21608 same embryo
	EMAGE:21606 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source