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EMAGE:29414

C330005M16Rik ( MGI:2142121)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29414
euxassay_006815_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8150
Entity Detected:C330005M16Rik, ( MGI:2142121)
Sequence:sense strand is shown

>T8150
GGAAGATGCTGAGGGCTCAACGGAGATGGATGGGAATCCCAGGATCGCGGCCTCCCTTGGGACTGAGGAC
ATCCTGACAGACGGACACACGGCTCTGCCTGGCATCGGTCCATTCTCCATCCTCTGTGGGACCCACCAGC
GGAATTTGATCACATCCCTGCATTCCACTCGGCTTCCCACCATCCCCTTTCTTCCTACCATGAGCCCCTT
TCTTGGAGGCTGGCTCTTCTTCTGCATGCTCCTGCCTTTCTCCCCGGGAGTCCAGGGAGCCCAAGAGTAT
CCTCATGTTACACCAGAGCAAATCCATCTGTCCTACCTGGGGGAGCCAGGCACCATGACTGTGACCTGGA
CCACATGGGCTCCAGCCCGGTCTGAGGTGCAGTTCGGCTCGCAGCTGTCTGGTCCTCTCCCATTCCGTGC
CCACGGCACCGCCAGGGCCTTCGTGGATGGAGGAGTTCTGCGGCGCAAACTCTACATTCACCGCGTGACG
CTGCGGAAGCTGCAGCCGGGGGCTCAGTATGTTTACCGTTGTGGCAGCTCTCAGGGCTGGAGCCGGAGGT
TTCGCTTCACAGCCTTGAAGAATGGGGTCCATTGGAGCCCCCGCCTGGCTGTGTTTGGTGACATGGGGGC
TGACAACCCGAAGGCCCTGCCCAGGCTACGGAGGGACACCCAGCAGGGCATGTTTGATGCAGTGCTGCAC
GTGGGAGACTTTGCCTACAACATGGACCAGGACAATGCTCGCGTTGGGGACAGATTTATGAGGCTCATTG
AACCTGTTGCTGCCAGCCTGCCATACATGACATGCCCTGGGAATCATGAACAGCGCTACAATTTCTCTAA
CTACAAGGCTCGCTTCAGCATGCCAGGGGACAATGAAGGCTTGTGGTACAGCTGGGACCTGGGGCCTGCC
CATATCATCTCATTCTCCACTGAGGTATACTTCTTTCTGCACTATGGCCGTCACCTGATTGAGAAGCAGT
TCCGTTGGCTGGAGAATGACCTCCAGAAAGCCAATAAGAACCGGGTGGCCCGACCTTGGATCATCACGAT
GGGTCATCGGCCTATGTACTGTTCCAATGCGGACCTGGACGACTGCACGAGGCACGAAAGCAGGGTCCGC
AAAGGCCTCCACGGCAAGTTGTTCGGGCTAGAAGACCTTTTCCATAAATACGGCGTGGATCTGGAGTTCT
GGGCTCATGAACACTCCTACGAGCGACTATGGCCAATTTACAACTACCAGGTATTTAATGGAAGCCTGGA
AAGCCCCTACACCAACCCTCGAGGCCCAGTTCACATCATTACAGGATCTGCGGGTTGTGAAGAGCTGCTG
ACTCCCTTTGTCAGGAAACCCAGACCGTGGAGCGCCGTGCGCGTGAAGGAGTACGGCTACACTCGGATGC
ACATCCTTAATGGCACCCATATGCACATCCAGCAGGTGTCGGATGACCAGGATGGGAAGATTGTGGACGA
CGTCTGGGTGGTTAGACCTTTGCTTGGCCGGATGATGTACCACTAAGACCGGGGTACCTCTTGTGAAGAC
ACTTTGTTGCTGTGACCAGGCAGTACCTTGGCCTGGGGAGGAAGTCAGGGCAGGACCCCACTCCCTCAGC
ATCCAGAAGCCTGAGGGCACAGCCTTGAGAGAGGCCAGACAGTTCTTGCCTCCTGGAGAGGTGGGAGCCC
GAGCTGGCTGGAGAGGGAGGGCAGGTATGTGGGCACCAGCAGGGCTGACTGCCAGCATAGCCCTGCCCTT
CTGAATTGCTCTGACAGGGCCAGGGACTCAAGGGCCTCAGAATAAAGAGGCTTGCTGCCATGGCTCCCTG
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30021730 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30021730 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006815 same experiment
 EMAGE:31381 same embryo
 EMAGE:31440 same embryo
 EMAGE:31477 same embryo
 EMAGE:31203 same embryo
 EMAGE:29525 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS