EMAGE mouse gene expression spatial database

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EMAGE:31477

Cntn1 ( MGI:105980)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006852_01	euxassay_006852_02	euxassay_006852_03	euxassay_006852_04	euxassay_006852_05

euxassay_006852_06	euxassay_006852_07	euxassay_006852_08	euxassay_006852_09	euxassay_006852_10

euxassay_006852_11	euxassay_006852_12	euxassay_006852_13	euxassay_006852_14	euxassay_006852_15

euxassay_006852_16	euxassay_006852_17	euxassay_006852_18	euxassay_006852_19	euxassay_006852_20

euxassay_006852_21	euxassay_006852_22	euxassay_006852_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

strong

regional

strong expression: see section 09 10 11 12 13 14 15

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 16 17 18

rest of cerebellum mantle layer

strong

regional

strong expression: see section 05 06 07 15 16 17 18 19 20

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

trigeminal v nerve

strong

regional

strong expression: see section 08 16

ureter

moderate

regional

moderate expression: see section 15 16 weak expression: see section 08 09

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 02 04 06 07 08 09 10 11 12 13 14 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 19 20

vagus x ganglion

strong

regional

strong expression: see section 07 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 16 17 18 19 20 21

kidney pelvis

moderate

regional

moderate expression: see section 07 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 15 weak expression: see section 16 17

telencephalon marginal layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

lateral ventricle choroid plexus

strong

regional

strong expression: see section 06 07 08 09 10 11 14 15 16 17 18 moderate expression: see section 05

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

4th ventricle

strong

regional

strong expression: see section 03 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

neural retina

moderate

regional

moderate expression: see section 01 02 03 21 22 23

spinal cord mantle layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15

mandible

strong

regional

strong expression: see section 17 18 19 20 moderate expression: see section 04 05 06 07 08 09 10 16 weak expression: see section 02 03 21 22

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 14 15 16 17

kidney calyx

moderate

regional

moderate expression: see section 05 06 08 18

pons ventricular layer

strong

regional

strong expression: see section 05 17 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 17 18

pons mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

hypothalamus mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 10 15 16 17

medulla oblongata basal plate mantle layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

maxilla

strong

regional

strong expression: see section 17 18 19 20 moderate expression: see section 04 05 06 07 08 weak expression: see section 02 03 21

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8110
Entity Detected:	Cntn1, ( MGI:105980)
Sequence:	sense strand is shown >T8110 GTCGCTATGTGGAGATCCAGCTTTGCAGTCTTATAACCTGTTCTTTGCAGAAGGTTGGCTGAAGGATGGG AGCGGCTATGCCTGCCTCTGAGGTCACAGCATGAGGTCCTTTTAACTTGCCTTCTGCTGCAGAAGAGAAG CCCATTGTGTCATAACTTTCCCTGAGTCTATTCAAAGGGGCAATTTGAAGCAGATATTATAGCAAGATCG GCAGATGATTCAACCCACACGGTCTTTCATAGTACTCAACTGCCATAAAGCTTTCATTACTTTTGGAAAC TGACCCATCTATTCAACAAAAGATGAAAATGCCATTGCTGGTCAGCCATCTCCTGCTAATCTCCTTAACA TCTTGTCTAGGAGACTTTACCTGGCACAGAAGATATGGTCATGGAGTGTCTGAGGAGGACAAAGGATTTG GGCCCATTTTTGAAGAGCAGCCAATCAATACCATTTACCCAGAAGAGTCACTGGAAGGAAAAGTGTCACT CAACTGCCGGGCACGCGCCAGCCCATTTCCAGTTTACAAATGGAGAATGAATAACGGGGACGTGGACCTG ACAAACGATCGGTACAGTATGGTCGGAGGAAACCTTGTTATCAACAACCCGGACAAACAGAAAGACGCTG GGGTGTACTACTGCCTGGCATCCAACAACTACGGAATGGTCAGGAGCACGGAGGCAACTCTGAGCTTCGG GTATCTCGACCCCTTTCCTCCCGAGGAGCGTCCGGAAGTGAAGGTGAAGGAAGGGAAGGGCATGGTGCTT CTCTGTGATCCTCCCTACCACTTCCCAGATGATCTTAGCTACCGCTGGCTTCTGAATGAATTTCCTGTAT TTATCACCATGGATAAGCGAAGATTTGTGTCTCAGACCAACGGCAATCTCTATATCGCAAATGTCGAGTC TTCTGACAGAGGCAACTACTCCTGCTTTGTGTCCAGTCCTTCTATTACAAAGAGTGTGTTCAGCAAGTTT ATTCCCCTCATTCCGATTCCTGAACGAACAACAAAGCCATATCCTGCTGATATTGTGGTGCAGTTCAAGG ACATATACACCATGATGGGCCAAAATGTGACTTTAGAGTGTTTCGCTCTAGGGAACCCTGTTCCTGATAT CCGGTGGCGGAAGGTGCTAGAACCAATGCCCAGCACTGCCGAGATAAGCACGTCAGGGGCTGTCCTCAAG ATCTTCAACATCCAGCTAGAAGATGAAGGCCTCTACGAGTGTGAGGCTGAGAACATCCGGGGAAAGGACA AGCACCAGGCAAGAATCTATGTTCAAGCGTTTCCTGAGTGGGTAGAGCATATCAATGACACTGAGGTGGA CATAGGCAGTGACCTCTACTGGCCTTGCATAGCCACAGGGAAGCCCATTCCTACCATCAGGTGGCTGAAA AATGGATACTCGTATCACAAAGGGGAACTGAGACTGTATGATGTGACTTTTGAAAATGCCGGGATGTACC AGTGCATAGCAGAAAACGCGTATGGGTCCATTTATGCCAACGCTGAGCTCAAGATCCTGGCTTTAGCTCC AACTTTTGAAATGAATCCTATGAAGAAAAAGATCTTGGCTGCGAAAGGTGGAAGGGTTGTCATTGAATGT AAACCCAAGGCCGCACCAAAACCCAAGTTTTCATGGAGCAAAGGGACAGAGTGGCTCGTCAACAGCAGCA GAATACTCATATGGGAAGATGGTAGCTTGGAAATCAATAATATTACAAGAAATGATGGTGGAATCTATAC ATGCTTTGCAGAAAATAACAGAGGAAAAGCTAATAGCACTGGGACTCTGGTGATCACAAATCCCACACGA ATTATTTTGGCCCCAATCAATGCTGACATCACTGTTGGGGAAAATGCCACCATGCAGTGTGCTGCGTCTT TCGATCCTGCCTTGGACCTCACGTTTGTGTGGTCCTTCAATGGCTACGTGATTGACTTTAACAAGGAAAT TACGCATATCCATTACCAGAGGAATTTTATGCTCGACGCCAATGGGGAACTGCTCATCCGAAATGCGCAG CTGAAACACGCAGGCCGGTACACGTGCACGGCGCAGACAATCGTGGACAATTCCTCGGCTTCCGCTGACC TTGTCGTTCGAGGCCCCCCAGGTCCCCCAGGAGGCCTGAGAATAGAAGACATCAGAGCTACCTCTGTGGC ACTGACTTGGAGCCGTGGTTCCGACAATCACAGCCCTATCTCTAAATACACGATCCAGACCAAGACGATT CTCTCAGATGACTGGAAAGATGCGAAGACAGATCCCCCAATCATTGAAGGGAACATGGAGTCAGCTAAAG CAGTGGATTTGATCCCTTGGATGGAGTATGAATTTCGAGTGGTGGCGACCAATACTTTAGGGACGGGAGA GCCCAGCATACCCTCAAACAGGATCAAAACAGACGGTGCTGCACCAAATGTGGCTCCCTCAGATGTAGGC GGTGGGGGCGGAACCAACAGAGAGCTGACAATAACGTGGGCGCCTTTGTCGAGAGAGTACCATTACGGTA ACAATTTTGGTTACATAGTGGCGTTTAAGCCATTTGATGGAGAAGAGTGGAAGAAAGTGACTGTGACTAA CCCAGACACTGGCCGCTACGTCCACAAAGATGAGACCATGACCCCCTCCACCGCGTTTCAAGTCAAAGTG AAAGCCTTCAACAATAAAGGAGATGGGCCCTACAGCCTCGTAGCTGTCATCAACTCTGCACAAGATGCTC CCAGTGAAGCCCCCACAGAGGTAGGGGTCAAAGTGTTATCATCCTCTGAGATATCTGTCCATTGGAAGCA TGTGTTAGAGAAAATTGTGGAAAGCTATCAGATTCGGTACTGGGCTGGGCATGACAAAGAAGCAGCCGCA CACAGAGTTCAGGTCACCAGCCAGGAGTACTCAGCCAGACTGGAGAACCTTCTTCCCGACACCCAGTACT TCATTGAAGTGGGAGCCTGCAACAGTGCTGGATGCGGACCTTCCAGTGATGTGATCGAGACCTTCACCAG AAAAGCCCCTCCTAGCCAACCACCAAGGATCATCAGTTCGGTGAGATCTGGCTCACGATATATAATCACA TGGGATCACGTTGTGGCACTATCCAATGAATCTACTGTGACAGGCTACAAGATACTCTATAGACCGGATG GCCAACATGACGGCAAGCTGTTCTCAACCCATAAACACTCCATAGAAGTCCCCATCCCCAGAGACGGAGA GTATGTTGTCGAGGTTCGAGCACACAGCGACGGGGGAGACGGAGTTGTGTCTCAAGTCAAAATTTCAGGC GTGTCCACGCTGTCTTCGAGCCTCCTCAGCTTGCTCCTGCCCTCCCTTGGCTTTCTTGTCTACTCGGAAT TCTGAATGTTACGACCTTGGCTGTTTTCATCCCTGCTCAGAGGATGCTCTTCATCATGGGATGACTGTAA TTCCTTCTAATCCCTGCGGCTCCATCCTAAGCCAAACAAATTAGACTCTAACAAGCTGTTCAACTGATTT CCCAACACATCACGACTGAGGCATTCAGGAACCCCTTCATCCAAAAGAATAACTTTCAAGTGGAAATAAA GTGATTTTTAACTCGTTCCAATATGCCTTATAAACCATTTAACCTGATTCTGTGAAAGTTGCATGACTTA AATCAATGGACAAGTTACAGTGTTCAATTCAATGCCCTAGGCTGTAGAGTGAAGGTCACCATACACAGGT GCTTTACATGTTTGACAAGTTGTGAAATATAATAGAGATTGAAATGTTGATTGTATGTAGTATGTGTTCG AGTAATGCAGTCTCTTGTATTAGCCTATATGTTTGGGTTCCTGCCTATTTTTGAAAGACCCTCATCATTC ATGATATTTTGAGTTTTCCATAAAATTAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30099512 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30099512 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006852 same experiment
	EMAGE:31381 same embryo
	EMAGE:31440 same embryo
	EMAGE:29414 same embryo
	EMAGE:31203 same embryo
	EMAGE:29525 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source