EMAGE mouse gene expression spatial database

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EMAGE:29532

E130307J07Rik ( MGI:2443239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6259
Entity Detected:	E130307J07Rik, ( MGI:2443239)
Sequence:	sense strand is shown >T6259 TCGACCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGTCGACCCACGCGTCCGCGGACGCGTGGGCG ACCCACGCGTCCGAAAGACTGTGGCCATGACCAACCCCTCCCTGCTTATAGGCAGATACCCCAAGGCAGT TTCTGGACCATGGACACTTCTTTGACCAAAAGGCAGCTAGACAGCATCTGTGCATGCTGAGGCCCTGCCC AAAGGACTTGGTAGGGCTTACTAGACTTTGAGCTGCAAGTACATTTTTTTTTTTGGACACCCCGTTGAAC ATCCTGAATCTGGGCAAGGCATTTTCCTCCCAGTCTTGACCTAACAGATAGCTGAGCCATGGCACTGAAA GGCCGAGCCCTCTATGATTTCCACAGTGAGAACAAGGAGGAAATCAGTATCCAGCAGGACGAAGAGCTGG TTATCTTCAGTGAGACCTCACTGGACGGCTGGCTGCAGGGACAGAACAGCCGTGGGGAGACAGGGCTCTT CCCTGCTTCTTACGTCGAGATTGTCCGTCCTGGCATCAGCACCAACCATGTGGACTATTCCAACAGCCCT GCAGGCTCCCTGGGCACCCAGGGGAGTTTGTATAGCAGCCCGAGTATGGCCAGTCCAGCCAGGAGTGGTG GGGGCAGCGGCTTCCTCTCAAACCCAGGAAGCTTCGAGGATGATGATGATGATGATTGGGATGACTGGGA TGATGGGTGCACAGTGGTAGAAGAGCCACTAGCTGGCGGCCTAGGTACCAATGGGCACCCTCCACTCAAC CTCTCCTACCCGGGTGCCTACCCCAACCAGCATATGGCTTTCCGACCCAAGGCACCCCTGGAAAGGCAGG ACAGCCTGGCGTCTGCCAAGCGGGGCAGTGTGGTAGGACGGAACCTCAATCGTTTCTCGTGCTTCGTACG CTCTGGAGTGGAGGCCTTCATCCTTGGTGATGTGCCCATGATGGCCAAGATTGCTGAGACGTACTCCATT GAGATGGGCCCACGTGGCCCTCAGTGGAAGGCCAACCCCCACCCATTTGCCTGCTCCATAGAGGACCCCA CCAAACAGACCAAGTTCAAAGGCATTAAAAGTTACATCTCTTACAAGCTTACACCCACCCACGCAGGCTC GCCTGTTTACCGGCGCTACAAACACTTTGATTGGCTGTACAACCGCCTTCTACACAAGTTCACAGTGATC TCAGTGCCCCACCTGCCTGAGAAGCAGGCCACAGGCCGCTTCGAAGAGGACTTCATCGAGAAGCGCAAGC GAAGGCTCATCCTTTGGATGGACCACATGACCAGCCACCCTGTGCTCTCCCAGTATGAGGGCTTCCAGCA CTTCCTCAGCTGCCTGGATGACAAGCAGTGGAAGATGGGTAAGCGCCGGGCAGAGAAGGATGAGATGGTG GGCGCCAGTTTTCTGCTCACTTTCCAGATCCCCACAGAGCACCAGGATCTGCAGGATGTAGAGGACCGCG TGGATACATTCAAGGCTTTCAGTAAGAAGATGGATGACAGCGTCCTACAGCTTAGCAACGTGGCGGCGGA GCTGGTGCGGAAGCATGTGGGGGGCTTCCGCAAGGAATTCCAGAAGCTAGGAAGTGCCTTCCAGGCCATT AGCCATGCCTTCCAGATGGACCCTCCCTTTAGGTCTGATGCTCTCAACAATGCCATTTCTCACACTGGCC GGACCTATGAAACCGTTGGCGAAATGTTTGCGGAACAGCCCAAGCACGACCTCTTCCAAATGCTTGACAC ACTGTCTCTCTACCAGGGCCTACTCTCCAACTTCCCTGACATTATCCACCTGCAGAAAGGTGCCTTTGCC AAGGTGAAGGAAAGCCAGCGCATGAGTGATGAGGGCCGAATGGCTCAGGAAGAGGCAGATGGCATTCGCA GGCGCTGCCGTGTGGTAGGCTTCGCCCTGCAGGCCGAGATGAACCATTTCCACCAGCGCCGTGAGCTCGA TTTTAAGCATATGATGCAAAGCTACCTGCGCCAGCAGATTCTTTTCTACCAGCGGGTAGGCCAGCAGCTG GAGAAGACTCTCCACATGTATGACCACCTCTGACAGACTCTGACCAGCTCTGCAGGGCCTCTTCCCACCT GTCCATCCCACTCCTGTCATTAGCAGTGGCTCTGGTGCCCGGGGCGGGTGAGATGAGTGGCCTGTTCTTT CTAGGAGAAGACAAGGGACTTTTGAGAAGTCCTGGTATCCCTGGAATACTTTCCCCTGACTTTAGAGATA GATGAATGGCAGTAGCCCTCTGAGCCAAAGCCTGGGCTGCTGGTCAGTCCCTAAAGATGAAGCTTCTCCT GAGCCTGTAGTGCACACTCATCTGGCCACCACACCCTGTTCATTCCGTGGACTCCATAGACTGCTGTGGC CTTGGCTGAGACATGGGTGACCAAGGCTAGAACTTGTCTGTCCTGGAAGAGCCACTTTTCAAGACAATAG ACTTAGCAGACAATGCAGAATGAGAGGCGACACTTAAAGATTCCTGGAAACTCCTCAGAGGACCACAGAA CTCTGCTGGTTAGGGGGACACTTCACGGAGTAGGTGACATTCCGGCAGTATCTTGAAGAGTAACAAGGAC CTGCAAAAGACAGTAAATGGAGTGAAGGACATTTTGGGCAGAAGAGCGATGTGGTCCCTCCTCTTACCAT TCCCAAAGCAAGTTTGGTGTCCTCTCTGTGGGTTGTGTATGACAGAGGGGAGGGACCATCCCTGCTCTGT GCTCTCTGGCTTTCCCTGTCCTACCAATGCCTCTGCAGGAGGGACTGCAGGCCAACTCCCTCCTATAGGC CTTGGATTCCTCAATATGTTGGTTGAGGGACACCTCCCTCCCCCACTTTTCCTTGATTAGGGGACCATGC AAAAACTTTCCCTTCTGTAGGCTCAAGGTGCTCAATCCAGGGAGCACTGGGCCCTGGTGGTGCCTTTCTG GAGGGGAACTTCCAAAAGTTACCAGCGTAACTCCCCATTCTCCTAGCAGGTCCAGGCACCTTTGTTTCTG TGGCTTGGTCTTCTCAAATTACTCTTGTTGCACAGTGGGAAGGAAATCTGACCGAAATCCCAGAAGCTGG GATCTCTAGACCCCCATGTGTGGCCAGACTCACTGGTCAGTTTGTGCAGTCACCCATCTATGTTTACATC CTCCAGGCATTGCTGCCCTGTAACCCTCAGGCCTGGGTGTGGCCCAGTAGCTGGTTGTTGGGAAGTGACC CTGCCCCCCCTTGCCTCCCTGGGAAGCCATGTTCTGCTCCTGGGTCCTGCTGCTTGACTCTTTTCTTGAT AAACCTTTTCCAGTTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3501525 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3501525 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003364 same experiment
	EMAGE:29879 same embryo
	EMAGE:32176 same embryo
	EMAGE:29537 same embryo
	EMAGE:31379 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source