EMAGE mouse gene expression spatial database

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EMAGE:32176

Fgd1 ( MGI:104566)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003476_01	euxassay_003476_02	euxassay_003476_03	euxassay_003476_04	euxassay_003476_05

euxassay_003476_06	euxassay_003476_07	euxassay_003476_08	euxassay_003476_09	euxassay_003476_10

euxassay_003476_11	euxassay_003476_12	euxassay_003476_13	euxassay_003476_14	euxassay_003476_15

euxassay_003476_16	euxassay_003476_17	euxassay_003476_18	euxassay_003476_19	euxassay_003476_20

euxassay_003476_21	euxassay_003476_22	euxassay_003476_23	euxassay_003476_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

moderate

regional

moderate expression: see section 07 08 19 20

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19 20 21

upper jaw molar

moderate

regional

moderate expression: see section 07 08 19 20

arm

strong

regional

strong expression: see section 01 02

upper arm

strong

regional

strong expression: see section 03

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 15 16 17

metanephros

moderate

regional

moderate expression: see section 09 10 11 12 13 18 19 20 21 22 23

lung

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

leg

strong

regional

strong expression: see section 02 03 05 06

axial skeleton

moderate

regional

moderate expression: see section 15 16 17

tail paraxial mesenchyme

strong

regional

strong expression: see section 14 15 16 18 19 20 moderate expression: see section 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6765
Entity Detected:	Fgd1, ( MGI:104566)
Sequence:	sense strand is shown >T6765 GTCGATTCTCATTCCGGGAGAAAGAGACCCCCCTCGGTGGGATCCGGCTGGCCGCTCGCCCCCGGCTACG GAAGTGACTGGTCATCTATTAGGAGAGCTCGTGCCTAGCGCAGCCCCCGACCCTCGCCTAGCCGCAGCCG CATAGACAGCGCTCCCCAGCGGGCCCCCGGCCCCGCCGAGCCCACTCCCCGCTTGGGGCCAGGGCTGGCG ATGCCTGGCGCGGCAACGGCGGTGGCCGCGGCTGCTGCTGCTGCTGCCGCCACGGCTGCTGGCCGGAGCC CGGGTCGGGGGCTCGAGTCTCCGCAGTGGGGCTGAGCCCGCAGCCCCGCCCCCCGAGCCTGAGGCCGCTG CTCCGCCGGGCGCCGGGCCTTCTCCGCCCGCGCCTCCGCCCCAGGAACTGGAGCCAAGCGGGGAGCCCGG GCCCCGCGCCCAGGCCCGGACCATGCATGGCCACCGAGTCCCGGGGGGCCCTGGGCCTTCGGATCCCGAA CGCTCAGCGGCAAATACCCCCGGTGCCGCTCCGCTGGCCTGTGCCGACTCGGACCCTGGAGCCTTGGAAC CCGGGCTGCCGGTGAGCAGGGGCTCAGGCACGGCTCTTGGAGGCCCTTTGGATCCTCAGTTTGTTGGACC CTCGGATGCCAGCCTGGGAGCGCCTCCAAGCTCCCGGGTCTTACCCTGCGGCCCTAGTCCTCAGCACCAC CGGGCCCTGCGCTTCTCCTACCACCTGGAGGGCTCGCAGCCTCGGCCTGGACTGCACCAGGGAAACCGGA TCCTGGTTAAAAGTTTGTCCCTTGACCCTGGCCAGAGCCTTGAGCCTCATCCAGAAGGTCCCCAACGGCT TCGCTCAGATCCAGGCCCTCCCACTGAAATCCCTGGCCCACGTCCTTCACCACTGAAGCGGGCACCGGGC CCAAAGCCACAGGTTCCCCCAAAGCCCAGCTACCTGCAGATGCCCCGGGTGCTTCCTCCTCCTGAGCCCA TTCCTCCACCACCATCACGCCCTCTGCCTGCAGACCCTAGAGTGGCCAAGGGACTCGTCCCCAGAGCTGA GGCCAGCACTAGTTCTGCAGCAGTATCATCATTGATTGAGAAGTTTGAAAGAGAGCCTGTGATTGTTGCC TCGGATAGGCCAGCCCCTGGCCCCTGCCCAGTTCCCCCAGAGCCAGCCATGTTGCCACAGCCACCCCCGC AGCCAACAGGGTCCCAGCTCCCTGAGGGTGAAGCTTCTCGCTGCCTGTTCCTGCTGGCTCCTGGGCCCCG GGATGGTGAGAAGGTGCCTAACCGGGACAGCGGCATTGACAGCATCAGCTCGCCATCCAACAGTGAAGAG ACCTGCTTCGTCAGTGATGATGGGCCCCCCATCCACAGCCTCTGTCCTGGGCCCCCCGCCCTGGCTAGTA TGCCAGTAGCTTTGGCCGACCCCCACCGGCCTGGCTCCCAGGAGGTTGACAGTGACCTGGAAGAGGAAGA GGAAGAAGAAGAGGAGGAAAAGGAAAGAGAAATTCCAGTGCCCCCGATGGAGAGACAGGAGTCTGTGGAG CTGACTGTGCAGCAGAAGGTGTTCCATATTGCCAATGAGCTCCTGCAAACTGAGAAGGCCTATGTTTCCA GGCTCCATCTCCTGGATCAGGTATTCTGTGCCCGGCTGCTGGAAGAAGCTCGGAACCGCAGTTCCTTCCC CGCAGATGTTGTCCACGGCATCTTCTCTAACATCTGCTCCATCTATTGCTTCCACCAGCAGTTCCTGCTG CCTGAGCTAGAGAAGCGCATGGAGGAATGGGACCGCTACCCACGCATTGGTGACATTCTTCAGAAGTTGG CACCCTTCCTCAAGATGTATGGCGAGTATGTGAAAAACTTCGACCGGGCGGTAGAGCTGGTCAACACATG GACAGAGCGGTCTACCCAGTTTAAAGTCATCATCCATGAAGTACAGAAGGAGGAAGCCTGTGGCAACCTG ACCTTGCAGCACCACATGCTGGAGCCCGTGCAGCGTATCCCCCGCTATGAACTTCTTCTCAAGGACTATC TGTTAAAGCTGCCTCATGGCTCCCCGGACAGCAAGGATGCCCAGAAGTCTTTGGAGCTGATAGCCACAGC AGCAGAACACTCTAATGCTGCCATCCGGAAAATGGAACGAATGCACAAGCTGCTGAAGGTGTATGAGCTG CTAGGGGGTGAGGAGGACATTGTCAGTCCCACCAAAGAGCTCATAAAGGAAGGCCATATCCTTAAGCTGT CAGCAAAGAATGGGACCACTCAAGATCGATACCTCATATTATTCAACGACCGCCTCCTTTACTGCGTGCC CAGGCTGCGGCTCCTTGGTCAGAAGTTTAGCGTGCGGGCACGCATTGATGTAGATGGCATGGAGCTAAAG GAGAGCTCCAACCTCAACATGCCTCGGACCTTCCTGGTGTCAGGAAAGCAGCGCTCCCTCGAACTGCAGG CCAGGACTGAGGAGGAGAAGAAAGACTGGGTCCAGGCCATCAATTCCACCCTCCTGAAGCATGAACAGAC CCTGGAGACTTTCAAACTGTTGAACTCAACAAACAGGGATGATGAAGATACTCCCCCTAACTCTCCAAAC GTGGATCTTGGGAAGAGGGCACCTACGCCTATCCGGGAAAAGGAAGTCACCATGTGCATGCGCTGCCAGG AGCCCTTCAATTCCATCACCAAACGCAGGCACCACTGCAAGGCCTGTGGGCATGTGGTTTGTGGGAAATG CTCTGAGTTCCGAGCCCGACTCATCTATGACAACAATCGTTCCAACCGTGTGTGCACTGATTGCTATGTG GCCTTGCATGGAGCACCTGGGAGCAGTCCAGCCTGTAGCCAGCATACACCCCAGCGCAGGAGGTCCATCC TAGAGAAACAGGCCTCGGTCGCTGCTGAGAACAGCGTCATCTGCAGCTTCCTGCACTACATGGAGAAGGG TGGGAAAGGATGGCACAAGGCATGGTTTGTGGTTCCTGAGAATGAACCCTTGGTGCTGTACATCTATGGA GCCCCTCAGGATGTGAAAGCCCAGCGCAGCCTGCCCCTCATTGGCTTTGAGGTGGGGCCTCCTGAGGCAG GAGAGCGGCCAGACAGGAGGCATGTCTTCAAGATCACACAAAGCCACCTAAGCTGGTACTTCAGCCCAGA GACAGAAGAACTCCAGCGGCGCTGGATGGCTGTTCTCGGCAGGGCGGGCCGTGGGGACACATTCTGCCCA GGGCCCACACTGTCTGAGGACAAGGAGATGGAGGAGACACCAGTGGCAGCCTCAGGAGCCACTGCTGAAC CTCCTGAAGCCTCCCAGACCCGAGACAAGACCTAGAGGGTTTGGGGGGAACTGGGAGTTTCTGCCCCACA CTCCAGCTGCCCATGTTCAATTGGGGGCTCCAGCCCACTCCCAATGCAGTCAATACTTGAATTCCCATCA AGGGCACTTTCAATCCCGAATGCTGGGTCATGGGTTTTTTAATTCATCTTTTCACAAAAGGTGGGCTTTT TAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3674293 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3674293 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003476 same experiment
	EMAGE:29879 same embryo
	EMAGE:29532 same embryo
	EMAGE:29537 same embryo
	EMAGE:31379 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source