EMAGE mouse gene expression spatial database

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EMAGE:29589

Gpr22 ( MGI:1920260)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017253_01	euxassay_017253_02	euxassay_017253_03	euxassay_017253_04	euxassay_017253_05

euxassay_017253_06	euxassay_017253_07	euxassay_017253_08	euxassay_017253_09	euxassay_017253_10

euxassay_017253_11	euxassay_017253_12	euxassay_017253_13	euxassay_017253_14	euxassay_017253_15

euxassay_017253_16	euxassay_017253_17	euxassay_017253_18	euxassay_017253_19	euxassay_017253_20

euxassay_017253_21	euxassay_017253_22	euxassay_017253_23	euxassay_017253_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9982
Entity Detected:	Gpr22, ( MGI:1920260)
Sequence:	sense strand is shown >T9982 GCACTGCATCTCTCTGCAGTGGGGAGAAGCGACAAGGTAGGCTGTCACTCTGCAAAAGCTGCTCGGAGCA GGCTTCCCTCTCCAGAGCAGTGCCCTTCCTTTTCAGAAAGCCTGGATCCGAATGCTGTCTCAGGAGACAA GAAGCCTTCAGTATGCTAAATTACTTTCATTACGCATTTTCAGATGCTTATTGACTCCACAGTAGGAAGA GTGAGAGGCTGCTGCAGCCGAAGCAGCACTACATATTCTATACATTAGCAGGGCTGCTGAAACAATGGCA CAGTAGATACATTTCCATTAAGGTCGCTTGGAAGAGATTTTTATAACCCTTTTCCCCCAGCCCTTGTACT TTCAAATCAACAGAATGAATCAATCAAAACTGGAAATGCTTCAACTACATCAGGAACTCGTCAGCTCTTT AGCAGAGGGCCCACACCTAATTCAGTATACACAACTGACAATTTGAAGACTGCAACTGACTGAGAGGCTC GCTAGCCTCTGAACCACAGACTACCAGATATTCTTGTAACACATGATTTTAAGACACTAAGGAGTTAAAG CCTCAGATTAGCTGTGCTGGAACGGAACAAGCTGACAGCATCCCAAGACGATGCTACATAGACTAACCAC GAAAAGAAAAAAAATAGAACTGTAAAAGCCTGAAGACCAACTGGTGAGCGTTTTAAAAGGAAGATCTCAC ACTTTAATATAGCAGAATTATGGACACTATTGATTGTGCAAGAAACAGTGAAGCAAAAACTTCAAAAAGG AGAATGTCATGATGTGCCAGCAAGCAAAACAAAGCTTTAGCAAAGGTTTTTCTGCACTATTTAGATATTC ATATCAATTGGTATGACTAATAGAGTTTTCCATCTGAGCTTTATGACCAATTATGCATCCTCTTCTAATG AGAACAAACCGAATTAAATAGCAGATGGTTTTTATCAAAAGAGCGTGTACTGGATTTATAATATAAAGCA AATTATGTGATCAAGAAATCATTTCCAAACAATGAGACCTGCTGCAGACAGAGCCTGAAAACAAATGGAT ATCTAAATGCATTTTAGAAGCTAGAGACGGGATGTTTCCTTTTCTATTTTCACATATTCTGGAAAATAAA GAGATACAATCTTTTATTTCTTCAAAAAGAAAATGCAATTTCTATCATTTGATGAAGTTTCATTTGCCTG AAAATTATGTGCTGTTCTTTAGATATCCATTAGCCAACTAAATCTCATCATACAACTCAAGTACTCAAAT AGCAAATGAATGAATTCCTACAAAACATGAAATAAAACACAGCTACTCCACTGTTACCAGGAAAAAAAAA TTCATGTCAGAATTGTCAATGACATCATGTATCAATTTGTGGAAAACTACCATGGTGAGCCCAAAGGCCC ATAAAGCAACAGTAAACAGGCAAAACACCAACTGCTCCAAAAGAATGTGTTTTTCTCCTGTTCTGGAAAT CAACATGCAGTCTGAATCAAACGTCACGGTGCGAGATGACATTGATGACATCGACACCAATATGTACCAA CCACTGTCATACCCACTAAGCTTTCAAGTGTCTCTCACTGGATTTCTCATGTTAGAGATCGTGCTGGGGC TTGGCAGCAACCTTACCGTCCTGGTACTTTACTGCATGAAATCCAACTTAATCAACTCTGTCAGTAACAT TATTACAATGAACCTCCATGTACTTGATGTCATAATTTGTGTGGGATGCATTCCTCTAACTATAGTGATC CTTCTGCTCTCACTGGAGAGTAACACTGCTCTCATCTGCTGTTTCCACGAAGCTTGTGTTTCCTTTGCAA GTGTTTCGACAGCAATCAACGTTTTTGCTATTACTCTGGACAGATATGACATCTCTGTAAAACCTGCAAA CAGAATTCTGACAATGGGCAGAGCTGTAATGCTAATGACATCCATTTGGATTTTTTCTTTCTTCTCATTC CTGATTCCCTTCATTGAAGTAAATTTTTTCAGTCTTCAAAGTGGAAATACATGGGCAAACAAGACACTGC TGTGTGTCAGTACAAGTGAATACTATACTGAGCTCGGGATGTACTATCACCTTTTGGTGCAGATCCCCAT CTTCTTCTTCACAGTTATAGTCATGTTGATCACATACACTAAGATACTCCAGGCTCTTAACATCCGCATA GGCACTAGATTCTCAACAGGACAGAAGAAGAAAGCCCGAAAGAAAAAGACAATCTCTCTAGCTACACATG AGACCACAGACATGTCACAAAGCAGTGGTGGGAGGAATGTCGTGTTTGGTGTGAGAACTTCAGTTTCTGT AATAATTGCCCTCCGGCGAGCCGTGAAACGCCACCGGGAACGACGAGAACGGCAGAAAAGAGTCTTCAAA ATGTCGTTATTGATTATTTCTACATTTCTTCTCTGTTGGACACCAATTTCTGTTTTAAATACCACCATTC TATGTTTAGGCCCAAGTGACCTTTTAGTAAAATTAAGATTGTGTTTTCTAGTCATGGCTTATGGAACAAC GATATTCCACCCTCTCTTGTATGCATTCACCAGACAAAAGTTTCAAAAGGTCTTAAAGAGTAAGATGAAA AAGCGAGTTGTTTCCATAGTTGAAGCTGATCCCATGCCTAATAACGCTGTAATACACAACTCATGGATAG ATCCTAAAAGAAACAAAAAGGTTACCTATGAAGACAGTGAAATAAGAGAGAAATGTTTAGTACCTCAGGT TGTCACAGACTAGAGAGAAGTCTCAGCGCCACCAAATACATTCAGAAGAGTTTTAAATTTAAATTGTAAA AATGAAATTACTGCCAAATGTTAGAGAAAACATTCTAGGTATTGGTTATGTTGTAAATTTTCAATGTGAA TGTCAATTAGATTAGGTCATATATATATACTCAATTTCTTCATTATTTAATGTATTTGTTGCATGGCAGT TTGTTAAAAAGTAATATCATGTGTATATTTTGTCAATATTATGTCCATCAGAAGATATCCATGTAAGTCA TATTTTCTAAAGAATAAATATGTAGCCTTAAAAGAGTGTATAACTTTAAAATGTAACTGACACAGGTATC TACGTTTTTAAAAACAATGTATGAGGTCTATTATTTCTAAGCCACGAACTACAGATATATTTATATGGTG ACAAGTTGCATAAAGTATTTAACACAAAAAAATTATAGGCTCAAAAAAATCAAAGCAAGATTTTCTGTAC CACTATGCTATGTTTCTGATAATGACCAACTTGTTATAATATTTTAACAATGAAATAATGAACTATATAT AAACCTTATTTCTTTTGAATGTAGAAAATGTAGACTATATTAGTATTTCAAAACCATAAGTCAATGAATA AATAAATCCTATATATGCACACAAAAATAGGGAAACTTTAAAATTCAAGTTTACTATAAAAAGAAGAGAC TGAATTCTATTACAAGGGTAATATGCCTACTATACAAAGGAACCAACAATAAAGGGAGGGAAGAAAGCCA CAGTAACACCAGCCTTATTCCTCCTTCCTCATTTGGCCTGATTCTACAGCAAATGTCCTAGGCTAAAAGA AAAGACTGGTCTTAAAAGCCTGCAATAAAGAAATTTTTCACTTAACTCTACACTATTTTGTATACAAGAC GTTAACCACACAAATAGGGAGACTGAGAAGAAGATGAGAACCATTTCTGTCACTGTAGAAAAGCCTAACA CAGCCAGATGTTTCTAATTTTGGATACATAGCAACTTATGTGAGGCAGAATTTATACAACATTCCTTCCA AGCCTTCAAAATACAGCAGCTCTGTCTCCAGTCTCTAGGAGATTAAACTAATAATAAATTGTGTCACATT TCTGTTCCTCCACCATGTAACTTAGACATTATTCTCTTAAATCCTCAATTCTTATTGGAAAATTATTATT CATTGTAATTATAGATTGTAATTATAGATTGCTATTTTCATAGCAAACAAGAATTTCTTACTTGTTATAA TAATCTGGCAATGCATAGTAATACCAAAACTTAGCAACAGCTTATTTAAAATAGGTAAGTACCTGCTATA TATAACGAGATAGCTTGACAATAATTATTCTATATTTTAAATACTATATGAACTAAATGAAGTAACATAC TTTTTAAATTTAAAAGATCATGTTAAACAAGTTAATATGCATCACTTTTTACACAATGGTGAGACTCATC TAATCAACCTCACTGACCTGCCTCGTCCCTGGGGGAATGAATGTGATACTGCAGCTTGAAATCACTAAGG TTTCTGGAAAATTAAATTGATTTATTTTGATTATAATACTAGAGTGTCAAAACTTGGCAAAGGCCTACCT TTCTTCTGCTTTATGAAATTTTAAAAAATGCTACAGTTTGATAAATTGTAGCATAATCAAATAAAATTTA TTTTTGAAACCTAAAATTAAAACTTAATGGTGTTAAGAATTTTTTCACTGAATGTAAACATTTGGCCTAC AATATATCCTAACTGTTAATTAGAACTTTTAATGCATTTTGTACTTGGACTTCCAAGCATAAGTTAAAAT ACTAACACCTAGATTTATAAAGTCTTGATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30252116 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30252116 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_017253 same experiment
	EMAGE:29609 same embryo
	EMAGE:29571 same embryo
	EMAGE:29597 same embryo
	EMAGE:29563 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source