EMAGE mouse gene expression spatial database

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EMAGE:29597

Gm185 ( MGI:2685031)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_017245_01	euxassay_017245_02	euxassay_017245_03	euxassay_017245_04	euxassay_017245_05

euxassay_017245_06	euxassay_017245_07	euxassay_017245_08	euxassay_017245_09	euxassay_017245_10

euxassay_017245_11	euxassay_017245_12	euxassay_017245_13	euxassay_017245_14	euxassay_017245_15

euxassay_017245_16	euxassay_017245_17	euxassay_017245_18	euxassay_017245_19	euxassay_017245_20

euxassay_017245_21	euxassay_017245_22	euxassay_017245_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8627
Entity Detected:	Gm185, ( MGI:2685031)
Sequence:	sense strand is shown >T8627 CTGGCCGTAAAGCAGGCTCGAGCCGAGCAAGCCAAGGCCGCGGACCAAGCTGCTATGGCGGACATGGAGG ACCTCTTCGGGAGCGAAGCTGAGAGCGAGGCCGAGCGCAAAGATTCCGAGTCTGAGTCCGACTCGGACTC TGACCAGGACAACGGCGCTTCTGGCAGCAACGCCTCTGGCAGCGAAAGTGATCAGGATGACAGAGGTGAC TCCGGACAGCCCAGTAACAAGGAACTGTTCGGGGACGACAGTGAGGAGGAGGGGGCCTCTCATCACAGTG GCAGTGACAATCACTCTGAACGGTCAGACAATAGGTCGGAGGCTTCCGAGCGCTCTGACCACGAGGATAA TGAGCCCTCTGACGAGGACCAGCACAGTGGCTCTGAAGCCCACAACGATGACGACGATGAGGGCCACAGG TCGGACGAAGGGAGCCGTCACTCAGAGGCAGAAGGGTCTGAAAAAGCCCAGTCAGATGATGAGAAATGGG ATGGAGAGGATAAAAGTGACCAGTCAGATGACGAGAAGCTGCAGAACTCTGACGACGAGGATCGGGAGCA AGGGTCCGATGAGGATAAGCTGCAGAACTCTGACGACGACGAGGAGAAAATGCAGAACACAGATGACGAG GACCGGGCCCAGATTTCTGATGATGACAGACAGCAGCTGTCTGAGGAGGAGAAGGGGAATTCTGACGATG AGCATCCAGTAGCTTCTGATAATGACGAGGAGAAGCAGAATTCGGACGATGAGGACCAGCCGCAGGTGTC CGATGAGGAGAAAATGCAGAACTCAGACGATGAAAGGCCACAAGTCTCAGATGAAGATGGAAGGCGTTCA GACGGTGAGGAGGAGCAAGACCAGAAGTCAGAATCTGCAAGAGGCAGTGACAGTGAAGATGAAGTTTTAC GACTGAAGCGCAAGAACGCGATTCCATCGGATTCCGAAGCAGACAGCGACACCGAGGTGCCCAAAGGTAG CCCGTTTACCTTGTATGCAGGTCTGCTTCATAGCTCTCTGTGCTTGTAACTGTCGTGCCCTGGCAGACCT TGAGACTGCAGGACTCACAGAAGACTGTGTGTGTGTGCTCAGTGTACTCTGCAGCTCATGCTGTTAGGAA CCCGCTTCGTTGTTACAGTTTGTTGCAGTGAATTTATCTGAGCCTCTGAACGGTTTGTAAGTGACAGTGC TGCAGCCTGATTAAAGATAACCCTCTTATAACCCAGTGCTCCAGCTCCCCAGAGTTTTCTAAGGTGTAAA ATGGGGTGATGCAGACCCTCTTACAGAGCGGTGAGAATTAGGTAGGCTGTGTCCGTTGTTGACTGATGTT TCCTGAGGCTCTGCTGTACATGGCTGCCGTTCTAAGTCATGTCCTATACCTTGCCATCTGGTGGTGAGGG TGTGCCTACTCTGAGACAAGTGAACCACATACGTGTACAGTGGCTGGCTGGTGACATGAGCTAAGGGGAC TATGGGAAGGACATGGCTGTGCTCACCTCCTAAACAGGAAGTCGCATAATGAGCGTCCAGTACTCTGGTC ACCTTTGCTTTGCTGTCCAGTGGCATGTCTTTTGCCACGTCTCTCTAACTCAGTCTCATAGGTTTATTCT AGACCATGTGACTACCAATAATTCTGGCTTGTTTAGACAGCTGGCTCTGAAAAAACTCTTCTGTCTCCTG TTTCCTTGCTGTCGTGCCTCACCTTGCTTTGCTGGCTTACGCACATTATTGTCTTGATGGGTGCCTCAGT GACTCTGCTTGTGAGAATGAGACCTCGCTAGAAGGGGAAACTGGAAGAAGGATGCAGAGATGGAGGGGGA AAAAGAGCTTCCTGTGCAGTGTGCAAACCTCTCCACACACATCTTTCCATGTGTGTGCCACAGTGACTTT CTCTCTCCACGCACATGCCACAGTGCCTGTCTGTCCACGCACATGCCACAGTGCCCTTTGCCTCCCTACC CAAGCTCAAGATCTGGATTTATGCCATTTCTTGGAGCTGGCCCAGAAATCTTAGGGATGTTCAAATGAAG ACCAGATGTTAACACTCTGTGTTTTTCCCTCTGCACTGAAGATAATAATGGAACCATGGATCTGTTCGGA GGTGCAGATGACATATCTTCAGGGAGTGATGGAGAAGATAAGCCCCCAACTCCAGGACAGCCTGTGGATG AAAATGGCTTGCCCCAGGATCAGCAGGAGGAAGAGCCCATACCTGAGACCAGAATAGAAGTGGAGATCCC CAAAGTAAACACGGACTTGGGCAATGACTTATATTTTGTTAAACTGCCCAACTTCCTCAGTGTAGAGCCC AGGCCTTTTGATCCTCAGTATTATGAAGATGAATTTGAAGATGAGGAGATGCTGGATGAAGAGGGGAGAA CCAGGTTAAAATTGAAGGTAGAAAACACTATAAGATGGAGGATACGCAGAGATGAAGAAGGAAATGAAAT TAAAGAAAGCAATGCGCGGATCGTCAAGTGGTCAGATGGAAGCATGTCCCTGCATTTAGGGAATGAAGTG TTTGATGTGTACAAAGCCCCACTGCAGGGCGACCACAACCATCTTTTCATAAGACAAGGTACTGGGCTGC AGGGGCAGGCCGTCTTTAAAACGAAGCTCACCTTCAGGTAAGCGCTCTTGGTCCTGCTGCTTGGTTTGTG AAGCCTGGCCCCTCCCCGGCCCCACCCCGCCCCACCCCGCCCCCAGTGGAGCTCTGTAGCTCGGTGGCTC AGAGGTTCTCAGTTCTGGAGGCTCAGAGGCTTTAGTCAAGGACAGCTGAGGCTGCGTTCTTGGAGGCTGT GATGTAAAACAGAAAGTTGGTATTTTAGTTGCCTGCCGAGAATCCTAAAGGGCTTGTCTGTGCTGGCCAA ATGTATCCTTTTGTTTTCAGGAAAACTGACTTAGAGACAAACTGATAATTAGGGCTTGTGTGTGCCAGTG CCAGAGGAAGGGCGCTGAGCTGGGGGATGTGAATACTGGGGCTGTTGGGGCAGGCCTCTGTGGGAATGTG TCCCCTGGGCGGGCACATGTAGTCATCCTCCCTTGGGGATAGGCAGACCCCCTTCCTGGGTGACACTGCT CTGTAGGGAGCTCTGACCTTCAGAATTGTACTGCTTCAACTGTTTCTGCTCGTGACCTTGGACAGCTTTG ATTTTGTGAGATTGTGGGTAGCTGTGTGGTGTGGTGTGCACAACACTACTCGGGCCTGCCTGTTTGCTGC CTTGGGGTCATGCTGTACAACGGTCTTCACCCCACACCCCACACCTTTTTCTTTCCCAGACAGTTTCTCC GTGTAGCCCTGGCTGTCCTGGAACTCACGTTGTAGACCAGGTTGGCTTTGAACTCAGACATTTGCGGGGC TCTGCCTCCCAGGTGCTGGGCTCAAAGGGGCGCCACCCTGCCTGACGAGCTGGCCCCCGCTTTTCCTTTT CACTTTTCCCGTCTCATTGCCACCCCTGCCTGACAAGCTGGCCCCCACTTTTCCTTTTCACTTTTCCTGT CTCATTGAGCTCGAAAGGTGCCTTTCAACCTTCCCAGTTCCTCACGGTGTGGTGACTCCAACCATAAAAC TATTCATAACTGTAACTTTGCTACTGTTATGAATCGTAATGTAAATGTCTGATATGCAGGATATTTTATG CATGGCCCTGTATAAGGGTCGTGCAACTTCTGTGAAAGTGTCATTAGGCCCCTAAAGGTCACAACTGACA GGTTGAGAGGATTGACAGTGAATTAGAGGAAGGAAAGTGTTCTGTCCTCCAAGGAGAATATTCAGGTTAG AGGTCTGGCCTCTAAACTCTTCTTTGGTGGATAGGAAGCGGCTGCTGACTTTGTGTTTTTGTGATGTGGG TCTGATGGCTCACAAATATCCATGAGAAACAGTGGGAAGTTTCTGTTCATTTTCCACAGGCCTCACTCTA CAGACAGTGCCACACACAGGAAGATGACCCTGTCACTTGCAGATAGATGCTCAAAGACACAGAAGATTCG AATCTTACCAATGGCTGGCCGTGACCCTGAGTGCCAGCGCACAGAGATGATTAAGAAAGAAGAAGAACGC CTGAGGGCCTCCATCAGGAGGGAGTCTCAGCAGCGCCGGATGCGAGAGAAACAGCACCAGCGGGGGCTGA GCGCCAGCTACCTGGAGCCTGATCGCTACGACGAGGAGGAGGAAGGCGAGGAGTCCGTCAGCCTGGCTGC CATTAAAAACCGCTACAAAGGGGGCATCCGCGAGGAACGAGCCAGAATCTACTCCTCGGACAGTGATGAG GGCTCCGAAGAAGACAAAGCTCAAAGGTTACTCAAAGCAAAGAAGCTCAACAGTGATGAGGAAGGTGAAT CTTCTGGAAAGAGGAAAGCGGAAGACGATGACAAAGCGAATAAAAAGCATAAGAAGTATGTGATCAGTGA TGAAGAAGAAGAAGAAGATGACTGAGCTACAAACTGTGAATGCGTTTTATATATTTATTGTACAGCTTGT CTATAAATACAATGTAAACATGAGTTATTTTGATTGAGATTAATTGATCTGTTTGTGAAATTTTGATTGT AATAAAACATTTAAGGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5719017 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5719017 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_017245 same experiment
	EMAGE:29609 same embryo
	EMAGE:29571 same embryo
	EMAGE:29563 same embryo
	EMAGE:29589 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source