EMAGE mouse gene expression spatial database

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EMAGE:29604

6030405A18 ( MGI:3607715)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006105_01	euxassay_006105_02	euxassay_006105_03	euxassay_006105_04	euxassay_006105_05

euxassay_006105_06	euxassay_006105_07	euxassay_006105_08	euxassay_006105_09	euxassay_006105_10

euxassay_006105_11	euxassay_006105_12	euxassay_006105_13	euxassay_006105_14	euxassay_006105_15

euxassay_006105_16	euxassay_006105_17	euxassay_006105_18	euxassay_006105_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8541
Entity Detected:	6030405A18, ( MGI:3607715)
Sequence:	sense strand is shown >T8541 GGCAAGCGGGAGGTGCGCGCATTATGGGCAAATGTTTGAGTTCTCATTAAGACCGAAGAAAAAGCAACAC TCCCTCCTCCTGAGAGAACAGAGAGCCACATTCATTCGCAGAAACGTCTTGAAGACCAGTCTCCGTGGAC AAGTGATCCCTGCTGTTGCTGCCTTTGTTTAAAAACCAAGACGGTTTGTTGTGCTGACATCCTAGCCTGT GATTGCTGCTGCTGCTGCTGTCATGATCGAGCAATGTCAGTTTCGCCACTCTTAAGCCCTGTGTGAACCG CGGACTCCGGACGCGGACGCGAACACGGACTGGACGCGGACCAACGTTTGAGCGTGCCTGCTCACACCTC CCCTCTTCGTAGAGATGTCGGAGGCTGACTCATCATCTGGATTTGCCGGAAGTGTGGAGAATGGAACTTT CCTGGAGCTGTTTCCCACATCGCTGTCCACGTCGGTGGACTCGTCCTCGGGCCACCTGTCCAACGTCTAC ATCTATGTCTCCATATTCCTCAGCCTCCTGGCTTTCCTGCTTCTGCTGCTGATCATTGCTCTTCAGAGGC TGAAGAACATCATCTCTTCCAGCTCCTCGTACCCGGAGTATCCTAGTGACGCTGGGAGTTCTTTCACCAA CCTAGAGGTGTGTAGTATCTCCTCTCAGAGGTCGACGTTTTCAAACCTCTCATCCTGAAGGAAATATTCC AAATGAGAATGGCTTTGGTTTCTCCGTACTGGAGGTATTGCTTCATGAAAGAAATGAGCATCAGGGCAGA CCTGTTTTCCTCCTTCGTTTTTAAATTTCTTTTCTCCTGAGTATATGCTTCTTTTGTGAAGAGAGGAGCC ATGCTGGTGGAAATGCAGGTAAACTTTGAGGCATGGGATTCCACACAAAGTTCATGGGTCACCTGAAGCC TGCTCAGCACTGTGTTGGGCACCACGGACTCAGACTACAGGGGAGACTGGGAAGAGACACTTCCACCCTG ACTCTCTTACAAAAGACCGGGCACTGGAGACGAGCAGAAACTGGAAAGCCAAGTAGAGACTGTTTAGAAG ATATTTTCTGTATTTAAAAGATAAAGCAGAGACGGGGGGGGGGGGGGGGGGGGGAGGGAGAGGGAGAAGG AGAGGGAAAAGAGAGAGAGAAATTATATTTAACATATAACATTTCCAAAGGGAGGTTAAAATTGGCTAAA TGTTTTTATTTAATTTCACCTTAGCAAATGTCACGCATGATGAGGACTGTTCCAGGGGCGTGGAAAGCTT TGGCGACACAAGGAAAGTGTGTCATGAGGCAGTTGGCCCTGCTTTCTGCTCCTGGGTTTCCTCCTGGCTT GTGTTGCAGCCAGATTGCTCTCCGGCTATGGAATCCTTTGTGACGGTCACTGTTGCTATTACTAAGTGAA GCCCAGAAGAGGCCCAATTCATTTTAATTTCCTCACTATGGTTTCCATCCAGTGGGAATTAAATATAAAG ACATATTCGTTGAGTTGCATTTTATACAGTGATATTCCTAGCACTCGTGTGCTCCTTCATTTCCATCCTT TAAAGCTTTGCTGCACAATAACATGGCCTCTAAGGTGTCAGTGTGTGCTCATGTGATTTCTCGATCCTTA GGGACAATGTCGCTAATAGTCGTGTCATCCTCCACACACGGGGCCTGTCTCTGATGACTTGTTCATGTTG ACATAATGCTCTGGTTTTCTAAGCGGTGAGTTTATGCTAAAACTGCAGACATTGCAATTGGGTAGCTGCA GACACTGGGCGCTGCTATCCTGAGGCCACCCACGGGTGTCCATGAGATGAGGCACAGCTGTGAGCACAAG GCTGTGCAGGGAATTGATCAGAGCCAATGACAGCTTGTATGGATACGAATTTAAAATCAATGTGTAAAAT AAATGGTCCACAGACTGAACTCTGAGCTGGGTAACCTGAGTGCCGCCGAGCCAACATACAGGTCTTTCTT AGGCAAAGTAGTTATTCCCTGCTCACAAAATGTGATAATCTCAAACACTGTAACTCCCACACATAAACAC ACCCAGCGGATAGGTTGTGCAGTCAGGACACCCTGGGGACTGGAATGGGAGAAAGAGTGAAACATTTTCC AGGCCCTGCCTGACATCCTAGACACGCTGAGGGTTTAAAGTGGTCAAGACTATCAAAGGCTGCTTCCTTC TTTTTTCTCCTCAGTCAGATCGGGACACACTTAGCTACTTACATTTCCAAAGTATCTACAGACACCATCA GACCAGTGCAGATGCTGCCCTACAACGTGTAGTGAAAAATCTTGTGTCAGCTGTCATGTAACGTGTGACT TAATACATATTAAAATCTTGGACTGGAGAGGTTCTGCAGTTTGGGTGCAAACTGCCATTCTCTGAGTGCA CATTCTCTCTGTGGGCACATGGATGCGTGTAAATACGTACACGCGGTGTGTGCACAGACACGTGGATGCG TGCACAGGTTTTCAGGGAAACGTTGTGGGACAGCTATCAGGAATTCAGAAGCATCTGCAATGTCACTTAT GCTCTTCTGATCATTTTGGTTCTTTGGGTAAAGAGATTCTAACAAATAAAAGGACAAACATCCCGAATGA CAAGTCAACCTCGTCAGGTCCCGTCCTGCAGAGGTAACTTCATATTCTTTTCTCCTGGAATAGATTCAAA GACTTTAGACAGCAAAACTACCGAAGTCAAACTCACTTGCCTCTCTGCAGGTGCGCTGCCTAACTAGCCA CATCGATTCTTGTGCCTGGAGATAGGCGGAGCAATTAGTAGCAGAACTCACCGGTGGAAAAGAGGCATCG CCACTAAGCATTGTAGAACTTGGGACGAATCTGTCTTGTGGAAATGTTCAGAGAGACTGGAATACAGGTA TACAAATAAAGAAGTGGAGTCTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708109 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708109 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006105 same experiment
	EMAGE:29591 same embryo
	EMAGE:31553 same embryo
	EMAGE:31563 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source