EMAGE mouse gene expression spatial database

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EMAGE:29610

Stard7 ( MGI:2139090)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003089_21	euxassay_003089_22	euxassay_003089_23	euxassay_003089_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4353
Entity Detected:	Stard7, ( MGI:2139090)
Sequence:	sense strand is shown >T4353 AGAGGCGGACACAGGCGCACGGGACCGCTTCTGCTGTGGCCTGGGACGGCCACCGCTTTGCTTCCCGCGC CCTGGCCGCGGGGGCCCCGCGAGTTCCCGCCGTACACACAGACGGGCGGGCTGTCGGGACCGAGAGGGCC TCGTGCAACGGCGCTCTCCTCGGAAGCCACGCTACCTCAGCCGCTTCTGCAAAAAGGCATGAGGGACGGC TCCTGGCTTCTGGAGCGTGAGGTGGCCTCGGGGTCAACCCGTTAAGGGGACTGCGCGGACGGCCCGGCCT GCCTTCCCCCAGCCCTTCCTCTCCGGGGCCCCGCGGCAGGCGCGGGAATCCACAGAGGCCGACATGTTCC CGAGGAGGCCACCGGCCACCCTGGCCGCCTGGCTGGCGGGCGCGCGGGGCGGGGGCCTGCTGTCCGCGCT GGCCAATCAGTGCCGCTTCGTGACGGGCCTGCGCGTGCGGCGAGCGCAGCAGATCGCGCAGCTCTACGGC CGCCTGTATTCCGAGAGCTCGCGCTGCGCCCTGCTCGGACGCTTCTGGCGCCGGCTGCGAGGCCGCCCCG GCCATGCCTCTGTTTTGATGGCGGCGCTCTCCGGTGTTTTCGTATGGGACGAGGAGAGAATCCAGGAGGA GGAGTTGCAGAGGTCCATTAATGAGATGAAACGGTTGGAAGAAATGTCAAATATATTTCAGAGCTCTGGA GTTGAGAATTACCCTCCCGAACCAAAGTCCCCAGCAGGAGGGAATGAAAAGTCAAAAGACAAGGAAGAAC CCTGGGAAATGGTGATGGACAAGAAACACTTCAAGCTGTGGCGCCGCCCAATTACAGGCACCCACCTTTA CCAGTACCGAGTTTTTGGAACATACACAGATGTGACTCCCCGACAGTTCTTCAATGTTCAGCTGGATACA GAGTACAGGAAAAAATGGGATGCCCTTGTGATCAAGCTAGAGGTGATTGAGAGAGATGCTGTTAGTGGTT CTGAGGTTCTTCATTGGGTAACCCATTTTCCTTATCCAATGTACTCTCGTGATTATGTTTATGTTCGGCG GTATAGTGTGGATCAGGAAAACAATGTGATGGTCTTGGTGTCACGTGCGGTGGAACACCCCAGTGTGCCA GAGTCTCCAGAGTTTGTCAGGGTCAGATCTTACGAATCCCAAATGGTTATCCGTCCTCACAAGTCGTTTG ATGAGAATGGCTTTGACTACTTACTGACATACAGTGACAATCCACAGACAGTGTTCCCTCGATACTGTGT CAGTTGGATGGTTTCTAGTGGCATGCCAGATTTCTTGGAGAAGCTGCACATGGCCACTCTGAAAGCCAAG AACATGGAGATCAAAGTGAAAGACTACATCTCGGCTAAGCCTCTGGAAATGAGCAGTGAGGCCAAGGCCA CTGCTCCATCCCCTGAGCGGAAGAATGAGGGTAGCTGTGGCCCTGCTCGGATTGAGTATGCTTAAAGGCC TTTGGGATAAGGAAAGACAGGCACTTCCAGCCCTGCCTTGCTCCTTCAGCTCTGCTGCAGGACAGGGACA AGTCACACATGTTAGAAGACATCTGCTGTAACTGTCAGTGCAAGAGGATTTAGAACTGGAGTTAGATTTC ATCTGGAGCCCTATTGCTCTTTACCAAACAGAGGAGCATGACATTGTGGAGTTGTCATGTTCTCAGGCCA GCAGTTGTGAAATCCAGTATCGTGCTTACCTACTCTGGAACAACCTCTCATGTTAGGCCAGAAGGAGATG ACTCCTATCCGCCTCCTACGAGAAGCTTCCTTGCTGACATTCCAAATGCCACTGTTGGTTATGCAATGCT CTGGATATCCTTCAGTTCTGTAGGTCACTTGGAGAGTGGTTGGCCCCTGGTTCTCACTAAGTGGTTTTTT GGAGTGCTCTTGGTAACAGTCCTGTGGTGGTGTGAACAATTCTGGCTGAGCTAAGTGCCTTGTCCTCACC TTGCTTTATCTTTAAAGACATGAAGTTTGTACCAAGGGATCCTTCCCCCTGCAGACTACCATGACATTGA CTCATCTGGTGGAGGCAGCTTTCATCTTAACTGGCCTTTCTGTTTTCATGGGCTGTGCTCACTGTGTCAT GGCCTCTGCCGTAGCCTTAGCCCATTTGCTGGGAGCACCAGAGACAAACTCCTCTAGACGGTAGCTTCCT AAATGATGTCATCAGTGTTTTGATAGGATCAGTGGAAGTGTGCACAAAACATCTTACTTAAAAATAGATT TCTTTTTAACCCTTGTAGCAAATGGAGTTAGGTGGTTGAGCACAACTCTTCCCTTAGTCTTGTTTGTTTT GGCACTATTTTCCCACTGCAGATTGACTGCGCTGTCCTGGAAGGATAAAGGTGGTAGATGGTGGTTGTGC CATTTCCTCAGAGTTTTCAAAACTGTAAGCTGACATATGGGCCAACAGATGGAGTCTTGCCCAATACCAG TATGGTAGGGATGGGGCTTTTGTGACCTCTTGGTCTTGTAAAGCATTTCTCTCCAGAGCTGACATAGAAG TAATAGACACAGTTTGGATAAGATCACAACCAGATGCTAGGTGCCTGGCCTTGCTGGTTTCTTTATGGTG AATAGGGATCAAGTTTCACATCCTTCTCTCTCTTTTCCTGGAATTAATAGAGCTGACATTTTCTTTTCTT GCTTGCTACCTACCCTGTTGTGGTGTTAAAAGAGACTAAACCCAAGGTGAACATAATCTTTAACCTTCTG TTGCCTATTCAGTTCTGTGCCAAGGAAACAGCTAACTCCCCAGTGTTTCTGTCTTTTCGGTTGTTGGCAG AGCAGAGAGTGGCATTGAGAAGCCCCAGTGTCTCTCAGTGAGCATTTTTCCCTTCTTCCCAGGACCTGAC AGGAAAAGAAAAAATCTTTATTTGACTTTAACATCTGTAGCTGACTGCCTGTGAATCTGTGAGCAAGTGT AAGCATGTATGTGTGCGTGTGTGTGCTGGGGCTCAGGGACTGAGCAGTTACGTTTACCCTTTGTTTTGTG ATTTGACTCTGTACAGTTAACTTGTGTCATCATAATGAGTTTGCTCTTAACTGTGACATTTTTATCAAAG GTGTGAATAAATGCATAAAGATTGGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527790 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527790 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003089 same experiment
	EMAGE:29627 same embryo
	EMAGE:31380 same embryo
	EMAGE:31377 same embryo
	EMAGE:29585 same embryo
	EMAGE:31996 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source