EMAGE mouse gene expression spatial database

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EMAGE:31380

BC016201 ( MGI:2385303)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001193_01	euxassay_001193_02	euxassay_001193_03	euxassay_001193_04	euxassay_001193_05

euxassay_001193_06	euxassay_001193_07	euxassay_001193_08	euxassay_001193_09	euxassay_001193_10

euxassay_001193_11	euxassay_001193_12	euxassay_001193_13	euxassay_001193_14	euxassay_001193_15

euxassay_001193_16	euxassay_001193_17	euxassay_001193_18	euxassay_001193_19	euxassay_001193_20

euxassay_001193_21	euxassay_001193_22	euxassay_001193_23	euxassay_001193_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

limb

weak

regional

weak expression: see section 01 02 03 04 05 24

pectoral girdle and thoracic body wall skeleton

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14 17 18 19 20 21 22

head mesenchyme

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

upper jaw incisor

moderate

regional

moderate expression: see section 14 15

tongue

weak

regional

weak expression: see section 12 13 14 15 16 17

nasal capsule

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

axial skeleton

weak

spotted

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4348
Entity Detected:	BC016201, ( MGI:2385303)
Sequence:	sense strand is shown >T4348 AGCTGCTGGGGCACTGAGAAGGCAATTCCTGGCTAGCCGCTGACATCTGAACAGGTGTCAGGATGTTGGG CTGGGTCCAAAGGGTGCTGCCTCAGCCTCCGGGGACCCCTCAGAAGACCGTGGAGACAGCAGGACCACAG CCAGAAACAGAGTCGAAACCAGAGGCCAACCCACAACCGGAGCCAGAACCTCAGCAGGAACCAGAGCCAG AGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAGCCAGAACCTGAACC TGAACCAGTACCTGAAGAGGCTCCACCTGAGGTCCAGGCCCTGCCACCAGAGGAGCCAATGGAAGGAGAG GGAGAGGCTGAGGCTGGTCCCAGCCTTCAAGAGACCCAGGTAGCTGACCCTGCTCAGCCCACCTCCCAGG CCCAGGTTGCTGTTGCCAAGGTGAACAGGCCCAGCTCCTGGATGTTGAGCTGGTTCTGGAGAGGCATGCA GAAGGTCGTGCCACAGCCTGTCTGCAGCAATGGGGGCCAGAACCTGGCTGCCGGAGAGCGAGACCCAGAT CAGGGTGGAGCACAGATCCCAGAACCTTGTGGCACTGGGGACCCAGGATCTGCAGAAGCTTCCGGGACTC AGGACACTGAGCCCAGCCTTTGGCTGCTCAGGTGGCTTGAGCAGAATCTGGAAAAAGTGCTGCCCCAGCC CCCTCCGCCTTCCCTGGCCTGGAAAGTTGAACCTGAGGCTGCTGTCTTGGATCCAGATCCTCCAGGAACC CCTATGCAGATGGAGCCCACAGAGAGCCCCTCCCAGCCTAATCCTGGACCCCTGGAGCCTGAGGAAGAGC CAGCTGCAGAGCCCCAGCCTGGCTTCCAATCCTCTTCCCTGCCACCACCCGGGGACCCCGTCAGGCTGAT TGAGTGGCTCCTACACAGACTGGAGATGGCCCTGCCTCAGCCCGTGCTCCATGGGAAGGCTGCAGAGCAA GAGCCTGGCTGCCCTGGGATGTGTGACGTACAGACCAGGGCGACAGCAGCTGGAGGCCTCTGAAACAAGG GAGAGGGGACCTGGGGAGCCCATGGACAAACGGATGCATGGATGGATGAAGGACTGAAGGATGCCTCAAG AGAAGAACTTTAGGGGGCAGTGACTGCTAATCTCCACCCTCTTTGAATAGTGAGCCCCCTGCACCCCCAG GCAGGGCAGAGGAAACCAGAGTCATTAAGTGTAACCCCAGAGCTTCCTCTCAGGGCTCTTGGGCAGGGGG CAGCACCCAGATCAGGGCAAGGGCAAGGAAGACTTCCTGGTGTGAGACTACAGAGAGCCCTCCCTATTGA GGCCTCAGTGTCTGAGCATGCTCTCACAAGGGGAACCTGGCACCATCCACACTACACGGGACTCAGTTTC CCTATAAGTGGTTCTTCCAAGGCCTGCCCTAGTTCTCTCTCCCAAGAGTGAGGGACCACCACCGACTGTG ACATTGTAATTGACATTAAAGAGTGTGACTTTGCACCTCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4527347 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527347 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001193 same experiment
	EMAGE:29627 same embryo
	EMAGE:31377 same embryo
	EMAGE:29585 same embryo
	EMAGE:29610 same embryo
	EMAGE:31996 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source