EMAGE mouse gene expression spatial database

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EMAGE:29666

Rpl15 ( MGI:1913730)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000421_18	euxassay_000421_01	euxassay_000421_02	euxassay_000421_03	euxassay_000421_04

euxassay_000421_05	euxassay_000421_06	euxassay_000421_07	euxassay_000421_08	euxassay_000421_09

euxassay_000421_10	euxassay_000421_11	euxassay_000421_12	euxassay_000421_13	euxassay_000421_14

euxassay_000421_15	euxassay_000421_16	euxassay_000421_17	euxassay_000421_19	euxassay_000421_20

euxassay_000421_21	euxassay_000421_22	euxassay_000421_23	euxassay_000421_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3835
Entity Detected:	Rpl15, ( MGI:1913730)
Sequence:	sense strand is shown >T3835 TGGCCTCGAGCCAGATTCGGACGAGGCCAAGATGGGTGCATACAAGTACATCCAGGAGCTATGGAGGAAG AAGCAGTCCGACGTGATGCGCTTTCTTCTGAGGGTCCGCTGTTGGCAATACCGCCAGCTCTCTGCGCTGC ACAGGGCTCCCCGCCCCACCCGGCCTGATAAAGCTCGAAGACTGGGATACAAGGCTAAGCAAGGCTATGT CATTTACAGGATTCGTGTCCGCCGTGGTGGTCGTAAACGCCCAGTTCCTAAGGGTGCAACTTACGGCAAG CCTGTCCACCATGGTGTTAACCAGCTGAAATTTGCCCGAAGCCTTCAGTCTGTTGCTGAGGAGAGAGCTG GGCGCCATTGTGGGGCTTTGAGAGTCCTGAATTCCTACTGGGTTGGTGAAGATTCCACATATAAATTTTT TGAGGTTATCCTCATTGATCCGTTCCATAAAGCTATCAGAAGAAATCCTGACACCCAGTGGATCACCAAA CCAGTCCACAAACACAGAGAAATGCGTGGGCTGACATCTGCTGGCCGAAAGAGCCGTGGCCT
Notes:	The probe template was PCR amplified from IMAGE:420528 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:420528 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000421 same experiment
	EMAGE:29813 same embryo
	EMAGE:29717 same embryo
	EMAGE:29829 same embryo
	EMAGE:29664 same embryo
	EMAGE:32047 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source