Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29813

Tmem45a ( MGI:1913122)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813
euxassay_000365_22 euxassay_000365_01 euxassay_000365_02 euxassay_000365_03 euxassay_000365_04
EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813
euxassay_000365_05 euxassay_000365_06 euxassay_000365_07 euxassay_000365_08 euxassay_000365_09
EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813
euxassay_000365_10 euxassay_000365_11 euxassay_000365_12 euxassay_000365_13 euxassay_000365_14
EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813 EMAGE:29813
euxassay_000365_15 euxassay_000365_16 euxassay_000365_17 euxassay_000365_18 euxassay_000365_19
EMAGE:29813 EMAGE:29813 EMAGE:29813
euxassay_000365_20 euxassay_000365_21 euxassay_000365_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3833
Entity Detected:Tmem45a, ( MGI:1913122)
Sequence:sense strand is shown

>T3833
TGGCCTCGAGGCCAGAATTCGGACGAGGCCGCAAGGCACGTCCCCCACGTAGCCTCCTGCTAGCCACTTA
CTACTTAATTTTTTTTTCCCCAAGAAGCAATAAGCAACCCACGCTTGAATCTTTTTCTCTCCCTCCCCCC
CACCCCCCATGTGAGGCGAGGCCACATCACATTCAACACAGTTTAGTTTTCATGGGGCTTTGAGATCAAA
AGAACAGAAACAGCAACCAAAGCTCAGCTGCCTTCTGATCCTAACTGACAAAGTGGGGAGAGTAAGGTGT
GCGCAAACAGGACAAGTTGGGTCATGGGGAGTTTCAAAGGACATGCTCTCCCTGGGAGTTTCTTCTTCGC
CATGGGCTTTTGGTGGACTATGAAGAACATCCTGAAATCTGTCTACAAAAGGCAAACTCGAACCTGCTAC
CTTAACTCTAAAACATTATTACGTCGGACAGAGATTTGGGAAGGAGTTGTTGTGCTTTTAATGTCTCTCA
CTGGTATAGCTGGTGAACAGTTTATCTCAGGAGGACCTGCCTTGATCTTGCATAAAGATG
Notes:The probe template was PCR amplified from IMAGE:420323 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:420323 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000365 same experiment
 EMAGE:29717 same embryo
 EMAGE:29829 same embryo
 EMAGE:29664 same embryo
 EMAGE:32047 same embryo
 EMAGE:29666 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS