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EMAGE:29689

Bloc1s1 biogenesis of lysosome-related organelles complex-1, subunit 1 ( MGI:1195276)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29689 EMAGE:29689 EMAGE:29689 EMAGE:29689 EMAGE:29689
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euxassay_002617_11 euxassay_002617_12 euxassay_002617_13 euxassay_002617_14 euxassay_002617_15
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euxassay_002617_16 euxassay_002617_17 euxassay_002617_18 euxassay_002617_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5269
Entity Detected:Bloc1s1, biogenesis of lysosome-related organelles complex-1, subunit 1 ( MGI:1195276)
Sequence:sense strand is shown

>T5269
GTCCGCCGTGCCCACACCCAGCCAGACTCGACCATGCTGTCCCGCCTGCTCAAAGAACACCAAGCCAAGC
AGAACGAACGCAAGGAGCTGCAGGAGAAGAGGAGGAGAGAAGCTATCGCTGCAGCGACCTGCCTGACCGA
AGCGTTGGTGGATCACCTCAACGTGGGTGTGGCCCAGGCCTACATGAACCAGAGAAAGCTGGACCATGAG
GTGAAGACTCTACAGGTCCAGGCTGCCCAGTTTGCCAAGCAGACAGGCCAGTGGATTGGAATGGTGGAGA
ACTTCAACCAGGCACTTAAGGAAATTGGGGATGTGGAGAACTGGGCTAGGAGCATCGAGCTGGACATGCG
CACCATTGCCACTGCGCTGGAATACGTCTACAAAGGGCAGCTGCAGTCTGCACCATCCTAGACTAAAACC
CACCCCTCTTACTTCACCCTCCTGGACAGGAGGGAAACTGGTGAGCCACGAATAAAAACACAAGCTTCCA
TTCTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5359972 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5359972 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002617 same experiment
 EMAGE:31659 same embryo
 EMAGE:31686 same embryo
 EMAGE:31695 same embryo
 EMAGE:29634 same embryo
 EMAGE:29633 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS