EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29743

Ecgf1 ( MGI:3818630)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011522_01	euxassay_011522_02	euxassay_011522_03	euxassay_011522_04	euxassay_011522_05

euxassay_011522_06	euxassay_011522_07	euxassay_011522_08	euxassay_011522_09	euxassay_011522_10

euxassay_011522_11	euxassay_011522_12	euxassay_011522_13	euxassay_011522_14	euxassay_011522_15

euxassay_011522_16	euxassay_011522_17	euxassay_011522_18	euxassay_011522_19	euxassay_011522_20

euxassay_011522_21	euxassay_011522_22	euxassay_011522_23	euxassay_011522_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30988
Entity Detected:	Ecgf1, ( MGI:3818630)
Sequence:	sense strand is shown >T30988 ATCAGACCGGGAGTGGGTGCCGAGGTACTGGTCGACGTGGGCCAGTGTTTGTCCCGAGGGACACCCTGGC TCCGCGTGCACCTAGACGGCCCTGCGCTCAGCAGTCAGCAGCGACGCACTCTCCAGGGGGCGCTCGTGCT ATCAGATCGAGCACCCTTCAAGGTCCCTTCACCCTTCGCTGAACTTGTCCTCCCACCGACCATCGCACAG CCCTAAGTCTCGAACCCAGCTCAGCCAGGAGCTTCAGGTCCACGATGCTACTGGCTCTAGGGCCCAAAGC TTGGCCCAAGCTCTCTCAGTTCAAACCCCTCCTCAGGATCTCAGGAGGTGAAACTCTGCATAGAAATTCC CGACACTGGGCAGGGCAGGGCCAGCGCCAAGGCCCTGGGCTAAGAACTAGACTGCTGATCACAGCCCTGT TTGGGGCTGGGCTAGGCTGGGCCTGGCTGGCCGCGAGGGCTGAGAAGGAACAGTGGCGCCAACAGCAGCG TACAGAGGCCCTGCGCCAGGCTGCTGTGGGCCAGGGTGACTTCAGCCTACTAGACCACAAAGGCCAGCCT CGATGCAAAGCCGACTTCCGAGGCCAGTGGGTGCTGATGTACTTCGGTTTTACTCACTGCCCTGATATTT GCCCCGATGAGCTGGAAAAGCTAGTGCAGGTGGTGCGGAAGCTAGAGGCGGAGCCTGACCTGCCCCTGGT GCAGCCTGTCTTCATCACTGTGGACCCAGAACGAGATGACGTGGCAGCCATGGCCCGCTATGTGCAAGAA TTCCACCCAAGACTGTTAGGTCTGACCGGTTCTACAGAACAGGTGGCCCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4163864), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 35659. Forward Primer - name:035659_F_IRAV32-35_D03_Ecgf1, sequence:ATCAGACCGGGAGTGGGT; Reverse Primer - name:035659_R_SP6_IRAV32-35_D03_Ecgf1, sequence:ATGGGCCACCTGTTCTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011522 same experiment
	EMAGE:29744 same assay
	EMAGE:31816 same embryo
	EMAGE:29744 same embryo
	EMAGE:32003 same embryo
	EMAGE:29754 same embryo
	EMAGE:29834 same embryo
	EMAGE:31501 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source