EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29834

Gpatc8 ( MGI:1918667)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011499_01	euxassay_011499_02	euxassay_011499_03	euxassay_011499_04	euxassay_011499_05

euxassay_011499_06	euxassay_011499_07	euxassay_011499_08	euxassay_011499_09	euxassay_011499_10

euxassay_011499_11	euxassay_011499_12	euxassay_011499_13	euxassay_011499_14	euxassay_011499_15

euxassay_011499_16	euxassay_011499_17	euxassay_011499_18	euxassay_011499_19	euxassay_011499_20

euxassay_011499_21	euxassay_011499_22	euxassay_011499_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30986
Entity Detected:	Gpatc8, ( MGI:1918667)
Sequence:	sense strand is shown >T30986 GGTTCATCCTGCACTGGGACTCTCGGGTTGATGAGCAGCAGGCGGCTGCTGCTGTCAGGAGGATGCACAG TTGCAGTGGTCTCCCTGACGTGCTTTGTTATACAGAGAAACCGTGGACTCCATGGAGGAGGGGAGGATTG GTTCAACAGCTTTATTCTCTGGAAGCAGTGACACTCGGTGATTGTCTGGGAAAAGGGATCCTTTGGTTTC CCTGGGTACCTTGTTCCAGACAGTCTTATTTGCCTTCCTAGTATTTAGCCCCCTCTGACAATTTTTTAAA TGTGCCTTGTGGGTTGGCTAGACACTAAAATAGAAGTAGACCAGAGGATACTAGAGTTGGGACAGTTCTC TCTTTGTTGGGAGGTGAGCACGTTTATAGGTAGAGAGATTTTTGGCTTCGAAGTTCTGTGCCATCTGCCC AGCTGGATTGCTATTTCAGCAGTAGAGGTTCCTTTTTTTTTTTTTTTCCTCCAGTGACACTGACCAGAAG TCTGTCCATCCCTCTCCCCTTTGCCCTTCCCCCAGGCTCTCAGCCCTCCTTAACACTGACCCAAAGGTGC TTGTAATGCAGGTCTCATCTCCTTTGTTAGCGCTCTTCCTATGACTAGAACTGGTTAAGCAGTCAACTGA CGTTAACCCTGCTTGCCTAGGCCAAGGTTCTCTGTTCCTGCCTTCTCAAATTCCCAGCAGAGTGGGCTGT GTTAGGGAAGTGAGTGCTGGGCACTTGAGGCCCGTCCCTGCCTGAGCTTCTCCTACCTTCTGCTCATTGT CCCCTTACAAGTATCACTTTTAGGATAACTAGCCCTGGAAAAACGGTATCTGTTTGAAGTGAAGGGACTG GTTCTTCTTGCTTTCAGCCCGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3983946), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 21911. Forward Primer - name:021911_F_IRAV32-35_C18_5430405G24Rik, sequence:GGTTCATCCTGCACTGGG; Reverse Primer - name:021911_R_SP6_IRAV32-35_C18_5430405G24Rik, sequence:ACACGGGCTGAAAGCAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011499 same experiment
	EMAGE:31816 same embryo
	EMAGE:29744 same embryo
	EMAGE:32003 same embryo
	EMAGE:29754 same embryo
	EMAGE:29743 same embryo
	EMAGE:31501 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source