EMAGE mouse gene expression spatial database

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EMAGE:29792

Ciz1 ( MGI:1920234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006938_01	euxassay_006938_02	euxassay_006938_03	euxassay_006938_04	euxassay_006938_05

euxassay_006938_06	euxassay_006938_07	euxassay_006938_08	euxassay_006938_09	euxassay_006938_10

euxassay_006938_11	euxassay_006938_12	euxassay_006938_13	euxassay_006938_14	euxassay_006938_15

euxassay_006938_16	euxassay_006938_17	euxassay_006938_18	euxassay_006938_19	euxassay_006938_20

euxassay_006938_21	euxassay_006938_22	euxassay_006938_23	euxassay_006938_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6614
Entity Detected:	Ciz1, ( MGI:1920234)
Sequence:	sense strand is shown >T6614 CGCAGGGATAGCGGCTACTTGCAGAGAGCCTACGGGCTTTGTGGACAGGCGAGGCCACAGGATTACCACC ATGTTCAACCCGCAACTCCAGCAGCAGCAACAGTTGCAGCAGCAGCAGCAACAGTTGCAGCAGCAGCTCC AGCAGCAGCAGCTCCAGCAGCAGCAACAGCAGATACTGCAGCTCCAACAGCTGCTGCAACAGTCCCCACC ACAGGCCTCCTTGTCCATTCCTGTCAGCCGGGGCCTCCCCCAGCAGTCATCCCCGCAACAGCTTCTGAGT CTCCAGGGCCTCCACTCGACCTCCCTGCTCAATGGCCCCATGCTGCAAAGAGCTTTGCTCCTACAGCAGT TGCAAGGACTGGACCAGTTTGCAATGCCACCAGCCACGTATGACGGTGCCAGCCTCACCATGCCTACGGC AACACTGGGTAACCTCCGTGCTTTCAATGTGACAGCCCCAAGCCTAGCAGCTCCCAGCCTTACACCACCC CAGATGGTCACCCCAAATCTGCAGCAGTTCTTTCCCCAGGCTACTCGACAGTCTCTGCTGGGGCCTCCTC CTGTTGGGGTCCCAATAAACCCTTCTCAGCTCAACCACTCAGGGAGGAACACCCAGAAACAGGCCAGAAC CCCCTCTTCCACCACCCCCAATCGCAAGGATTCTTCTTCTCAGACGGTGCCTCTGGAAGACAGGGAAGAC CCCACAGAGGGGTCTGAGGAAGCCACGGAGCTCCAGATGGACACATGTGAAGACCAAGATTCACTAGTCG GTCCAGATAGCATGCTGAGTGAGCCCCAAGTGCCTGAGCCTGAGCCCTTTGAGACATTGGAACCACCAGC CAAGAGGTGCAGGAGCTCAGAGGAGTCCACCGAGAAAGGCCCTACAGGGCAGCCACAAGCAAGGGTCCAG CCTCAGACCCAGATGACAGCACCAAAGCAGACACAGACCCCGGATCGGCTGCCTGAGCCACCAGAAGTCC AAATGCTGCCGCGTATCCAGCCACAGGCACTGCAGATCCAGACCCAGCCAAAGCTGCTGAGGCAGGCACA GACACAGACCTCTCCAGAGCACTTAGCGCCCCAGCAGGATCAGGTAGAGCCACAGGTACCATCACAGCCC CCATGGCAGTTGCAGCCACGGGAGACAGACCCACCGAACCAAGCTCAGGCACAGACCCAGCCTCAGCCCC TCTGGCAGGCGCAGTCACAGAAGCAGGCCCAGACACAGGCACATCCACAGGTACCCACCCAAGCACAGTC ACAGGAGCAGACATCAGAGAAGACCCAGGACCAGCCTCAGACCTGGCCACAGGGGTCAGTACCCCCACCA GAACAAGCGTCAGGTCCAGCCTGTGCCACGGAACCACAGCTATCCTCTCACGCTGCAGAAGCTGGGAGTG ACCCAGACAAGGCCTTGCCAGAACCAGTAAGTGCCCAGAGCAGTGAAGACAGGAGCCGGGAGGCGTCCGC TGGTGGCCTGGATTTGGGAGAATGTGAAAAGAGAGCGGGAGAGATGCTGGGGATGTGGGGGGCTGGGAGC TCCCTGAAGGTCACCATCCTGCAGAGTAGCAACAGCCGGGCCTTTAACACCACACCCCTCACATCTGGAC CTCGCCCTGGGGACTCTACCTCTGCCACCCCTGCCATTGCCAGCACACCCTCCAAGCAAAGCCTCCAGTT CTTCTGCTACATCTGCAAGGCCAGCAGCAGCAGCCAGCAGGAGTTCCAGGATCACATGTCAGAGGCTCAG CACCAACAGCGGCTTGGGGAAATACAACACTCGAGCCAGACCTGCCTGCTGTCCCTGCTGCCCATGCCTC GGGACATCCTGGAGAAAGAAGCGGAAGATCCTCCGCCCAAACGCTGGTGCAACACCTGCCAGGTGTACTA CGTGGGAGACTTGATCCAGCACCGTAGGACACAGGAGCACAAGGTTGCCAAACAATCCCTGAGGCCCTTC TGCACCATATGCAACCGTTACTTCAAGACCCCTCGAAAGTTTGTGGAGCACGTGAAGTCCCAGGGACACA AGGACAAGGCCCAAGAGCTGAAGACACTTGAAAAGGAGACAGGCAGCCCAGATGAGGACCACTTCATCAC TGTGGACGCCGTCGGTTGCTTTGAGAGTGGTCAAGAAGAGGACGAGGATGACGACGAGGAAGAAGAAGAA GAAGGAGAGATTGAGGCTGAGGAGGAATTCTGCAAGCAGGTGAAGCCGAGAGAAACATCCTCAGAGCAAG GGAAGGGCTCTGAGACGTACAACCCCAACACAGCCTATGGTGAGGATTTCCTGGTGCCAGTGATGGGCTA TGTCTGTCAAATCTGTCACAAGTTCTACGACAGCAACTCAGAATTGCGGCTTTCTCACTGCAAGTCCCTG GCCCACTTTGAGAACCTGCAGAAATACAAAGCCAAGAACCCAAGCCCTCCTCCTACCCGGCCTGTGAGCC GCAAGTGTGCCATCAACGCCCGCAACGCCCTGACTGCACTGTTCACCTCTAGCCACCAGCCCAGCCCCCA GGACACAGTGAAAATGCCCAGCAAGGTGAAGCCTGGATCCCCCGGACTCCCTCCTCCCCTTCGGCGCTCA ACACGCCTCAAAACCTGATAGAGGGAGCTCTGGCCACTCAGCCTGACTAAGGCTCAGTCTGCTAATGCTT CCTAGGTATCTGTGTAGAAATGTTCAAGTGGTTGGTGTTTTTACTCAAAATCCAATAAAGAGTCAGTAGT TTGGCAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3597692 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3597692 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006938 same experiment
	EMAGE:31928 same embryo
	EMAGE:31909 same embryo
	EMAGE:32202 same embryo
	EMAGE:29789 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source