EMAGE mouse gene expression spatial database

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EMAGE:32202

Serhl ( MGI:1890404)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006967_11	euxassay_006967_12	euxassay_006967_13	euxassay_006967_14	euxassay_006967_15

euxassay_006967_16	euxassay_006967_17	euxassay_006967_18	euxassay_006967_19	euxassay_006967_20

euxassay_006967_21	euxassay_006967_22	euxassay_006967_23	euxassay_006967_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 20

diencephalon roof plate

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6756
Entity Detected:	Serhl, ( MGI:1890404)
Sequence:	sense strand is shown >T6756 GACCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGGGACCTGGAGGGAAAGTCCTGAGCGGGACGCA AGAGGCGGGGCGTGAGCTTCTGAGTCCTTCCAGTTCCTGTGACCTCCTCAGCGAGGAGTGACAGCCTCAC CGACCAGAGCAATGGGTTTGCACTCAGAGTTGAAGTTGGCTGTGCCCTGGGGCCACATTGCTCTCAAAGT CTGGGGCTCCCAGAAGAACCCTCCAGTTCTCTGCTTACACGGCTGGCTGGACAACGCCAACTCCTTTGAC AGGCTCATTCCTCTTCTTCCCCAAGACTTCTGTTACATGGCTATGGATTTCGGAGGCCACGGGCTCTCGT CCCATTACAACCCAGGCCTCCCGTACTACCAGCAAAATTTTGTGAGTGAGGTCCGAAGGGTGGCAACAGC CTTTAAGTGGAATCAGTTCACCCTCCTGGGCCACAGTTTTGGTGGCTGTGTGGGTGGCACGTTCGCCTGC ATGTTCCCTGAGATGGTGGACAAACTGATCTTGCTGGACTCAACGCCATTTTTCCTGGACTCTAATGAAA TGGAGAACATACTGACCTACAGGCGGAGAAACATAGAGCACACACTTCAGGTGGAGGCCTCCCAGAAAAA GTCCCTGAGAGCTGTCAGCCCAGAGGAGATGTTGCAGGGGTTCCTGAATAACAACAGCCACTTGGATAAA GACTGTGGGGAACTCATCCTACAGAGAGGAACCACGAAGGTGGACGCAGGTCTGGTGCTGAACAGGGACC GGAGGATCTCTTGGCCAGAGAACAGCTTTGACTTCGTAAGCAAGGAGATGTTCGTGCACTCTGCGAAGAG CCTGCAGGCCAGCGTGCTAATGATCAAAGCGCTGCAGGGGTACTACGATGTGAGGCGGGCGAATGATGCA GACAAGGCGCCTATGCACTTCATGGTGGACACACTAAGATCCACCCTAAAGGAGCGGTTCCAGTTTGTGG AAGTCCCAGGCAATCACTACATCCACATGAACAAGCCCCAGGTGGTGGCCGGAGTCGTCGGTCCCTTCCT ACAGGGCCTACAGAGGATGACTTCTGCCAGGCTGTAGCTCTGGGATCCTGTGCACTTTCTAGCTTCCACT GGATCTCGAGCACTCTCGTCCCACAAAACAGGTGGAGTGGGCGGGAGTCTTGCAGACCAAGAGAGGCAGC AGAGACTCCTTCAGCAGAGGTAGGGGCAGGGAAAAGAAGATTCTGGCCTTATCTGTGACTCTGACAAAGG GCCTGGGGTGTTTGGTGAGCTTTGGTGATGCTAAGGAAGCAGGGCTGGAGCCGACCCTCTGGGCTCTGAG GCTTCTGTGGCCTTGCCACCAGTGGTGGAAATAAAATCTCCCATCTTCAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3673247 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3673247 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006967 same experiment
	EMAGE:31928 same embryo
	EMAGE:29792 same embryo
	EMAGE:31909 same embryo
	EMAGE:29789 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source