EMAGE mouse gene expression spatial database

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EMAGE:29864

6720456H20Rik RIKEN cDNA 6720456H20 gene ( MGI:2443219)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_005698_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8790
Entity Detected:	6720456H20Rik, RIKEN cDNA 6720456H20 gene ( MGI:2443219)
Sequence:	sense strand is shown >T8790 CCCCGCTCGCTGCCTGGGGGAGACTCGGGGGAACTGATCACAGCCGCACATGAGCTTGGAGTCGCCCATC CCCCGGGCTATCCGTTGTTCACACTGTTGGCTAGCCTGACAATCACCCTCTTTCCATTTGGCTCCGTCGC CTACCGCGTCAATCTCCTCTGTGGCTTATTCGGGGCGGTAGCCGCCTCGCTCCTCTTCTACACCGTTTTC AGGCTTTCTGGCTCACATGCTGGAGGGATTCTTGCTGCGGGGGTGTTTTCATTTTCTCGCCTAACATGGC AGTGGTCCATTGCAGCCGAGGTTTTTAGCTTAAACAATCTGTTCGTGGGGCTGCTCATGGCGCTTACTGT GCGTTTTGAGGAGGCAACAGCCGCAAAGGAGAGATCAAAGATAGCGGCCATTGGTGCTTTCTCGTGTGGC CTCAGCTTGTGTAATCAGCACACGATAGTCCTCTATATATTATGCATAATACCCTGGATTCTTTTCCGAC TTCTCAAAGAGAAGGAACTCACCCTCAGCTTGCTGCTGAGGCTGACTCTGGCCTTCTCTGCTGGCCTGCT GCCCTATGTCTACCTGCCTGTCTCGTCCTACCTCAGTCGCGCCCGCTGGACCTGGGGAGACCAGACGACA CTGCGAGGGTTTCTGACACATTTCTTCAGGGAAGAATATGGAACGTTCAGCCTGGCCAAGTCTGAAGTGG GATCGAGCGTGTCTACAGTACTGCTTTCACAGGTAATAAATATGAAGACCGAACTTTCATTCAATATCCA AGCTCTTGCAGTTTGGGCAAATATATGTTTAGCTAGAAAGGACAGGCGGAAGTCATCAGTAGTATGGCTT TTCACTGGAATGCTTTGCCTGTATTCATTATTCTTTGCTTGGAGAGCAAATTTAGATATTTCAAAACCAC TTTTCATGGGTGTGGTGGAACGATTCTGGCTGCAGAGCAATGCGGTGGTGGCCGTTCTCGCTGGCCTCGG TTTGGCCACGCTTGTGTCTGAGACTAACCGAGTGCTCCACTGTACTGGGATTCGGAACCTGGAGTGGCTG TCAGCAGCCCTTTTTGTTGCTTATCAAGTATACTCAAATTACAGCATTTGTGACCAAAGAACCAATAACG TGATTGATCAGTTTGCACGAAATCTTCTGGACTCCATGCCTCAGGATGCCATCATCCTACTCAGAGGAGA CCTGCCTGGAAACGCTCTCCGTTACTTGCATTACTGCGAGGGCTTGAGACCAGATGTTTCACTGGTGGAT CAAGAAATGATGACCTATGAGTGGTACTTACCCAAGATGGCGAGGCATTTGCCAGGAGTGCACTTTCCTG GGGACCGGTGGAACCCTGTGGAAGGAGTGTTACCCAGTGGGATGGTCACATTCAACCTCTATCACTTTCT TGAAATGAATAAGCAAAAAGAAACATTTGTTTGCATAGGAATTCATGAAGGCGACCCAACCTGGAAAAAG GACTATTCACTTTGGCCATGGGGATCTTGTGACAAATTGGTGCCTTCAAAGATTGTATTCAACCCAGAGG AGTGGATTGAACGCACAAGAGCCATCTATAACTGGACTGAGGCCTATGAAAGATTTGGTCCATCTTCTTG GGAATCGGTGGCCAATGAGGAGATGTGGCAAGCAAGAATGAAGACTCCATTCTTCATCTTCAGTCTGGCA GAGTCTGCTGCAGTTCCTGCAGATGTGAAAGCACAACTCTACACGCATGCATATAAACTTTATAAGGAGA TTGTGTACTTGCAAGAGGAACACCCAGTGAATTGGCACAAGAACTATGCCATTGCCTGTGAGCGCATGTT ACGCCTTCCAGGGACCGGCATAGACCCTGAAGTCCTGCTGTCTGAAGCCATCAGACATTTTCATCTCTAC ACTCAGAAAGCACAAAATGATCCACAGCGAGCAGACATTATAGCTGCTCTCAAGCATCTAAGAAGAGAAC TGCAAAGTCTAAGAAATATAAAAAAGGTCTGAGCCAGCAAAGTGTGGTAGCCCTGCTTACCGCTCTGCTC CCAAAGTTTCTGAAGTCCAAGAATTTTTCCTTCAAGTACAGAAGCTGCATCAAAAAAGTAGAAATCTCAT TTATCTCCTGGATATCAGTGTCACGTGCAGCAGAAACGAGCTAATGGCTGAAATGCAGGCCTTATGCAAA ATCCTGTTTATCTCACGTTGCCAAATTAGCATTTCCATGAGAAGCATGCAAAAACATCTGTAGTCCTCAG AGCAACTGACTGTAGGTTCTAGCTGAGAAGGAACTGTCTCCACAGACATTGGAGACATGTCTCCATGCTT ATTGGGATCCAAGGGTAAATTCTTAAGAAATATTGCAGACCAAAGTCATTTTCTATTTCCTGCAAGTGAG ATAGTTTCTGAAAAACAATTCTTGTTATATACCTCATTACAATTCCTTTTTCCCTTGTTAACTATAACTA TTTTAGTCTTAAGTTTTATACTATATACCAAGTACCTGGTTTTTTATTTTTAACTTTGAGTTTTATCATC TTAGTTCTGCAGCCCATGAAAAGGAAAGCTAACTTCAAGTTCTCTCATTTCTGTTGCAGAAATGGGCAAA TTAAAAGGGCATTTTATATGTCCTTAATTAATTTCATATTTATGTTGTCCTTCTCTTTGGATTCACAGAT CTAGACCTAGTAATTAGGAAGTAGCTGCCCAGATGAACAAAGGGGCATGGGCGAGAGTGGGGGTGAGGAC GTCGTTCAATAAATTCATTTCTGTTTCAGGCATCTTTTATACATATTACTAAAGAGAACGTAGCAAGAAA TGCAGATAATAATAGCTCTTTATTTTATTATGACAGTTAATTAATAGCAACCTGTTTTAATGAATAAAGT CACTCAGAGTCCTTCAGCACTTCCTAAGTAGAGGCCAGAATCTGTAGGGATTCTTTGCCCCTGATTGTAT AGCTCCTAGACTCCGAGCACTATATAGGCCCCTGTATAGAAATGCTCACTAATGAAGAGGGAGGGCTAGA AGCTTGTCTGCATTCAAAGATCACTGGTGAGTCATTCAGCAAGAAAAGGCCCCTTACCAGGAATAGTCAC AGTTCCGTGGCATTGTACTAGCAAAAGGGTCTGATCAAAGGTCTCCCGTGGAGCTTGCATGGCTCCCTTT CATACCACGACCATAATTAAAACCACTAATTCTCTTTTAAAATGCTGCAGGATGCCATGTAGGCATCTGT CCGGAGTGTCCTTTGTGATGTCATAAGCGGTTAAGGACCAGTGCCGAGGGCTTTTGAGTGAAATGCCAGT CATGATGGTGCTTCAAGTCAAGGGTGCCTCTAAAAGCTTGACAGGGCCTTGACTGCACAATTCGAGCTGA ATTTGCCCCTTGTCAGCTGCCAGTAAATAAATCTCAAAGGGGGGAAAGCTGAAGTTTCATTACCTGATCC GTGGGGCTTTGTTGGTTTTGGCATCACACAGGGGAAGCTCTTGCTCCTCCATTCTCTGGATTTGAAGATG TCCATTGGAGCCTGCAGTGCCTGGACAGGGTTCAGAGCGGAACCTTTTGAAGAGTGTCAATAGTTGTAAC AGTTCAGCTGTTAGGAAGACAAATACATGGAGGGGCTCATTAATCCGCTTTTGGCTCTCGGTGCCTTTTG CCCTTTTATCACAGCCTTATTAGGCTCCTGCTCATCTTGAACCAGAAAAAACCGAATTGAAGTTGTTGAG TACTAATTGGCAAAGACTTTTAATCATGGGCCAAGAACTTTCACTGACTTGAAAGTAACTTCTCCGCAGG GAAGAGCCAGAAACCTGGTTCACCTTAAAACAAAAACCTGTTGGAGCTCAGGGCGGTGTGAGGCTGGGAG GAAGCACTGGGAAATTGTCTACGGTTCCAAAGAAATGATAGGAGGTTATATCCGCCTTTCCCTTTCCTGG GAGAGAAGGGCAGTAACATCCAAGCATTTCTTCAGAGAGCCTTTATTGGTTCAGAAAAACCAATTCTTGA GTGTGTTTGTTTCCGAACATCCTGTATGTGTGATTATTCATCTGAGACTCTATGTATACACACGGTATTT ATTTAAAAGGAACAGTGTCTGTCTAGGCTCACAAGGAGCAGCCGAGGCCTCTTTGAGAACACCTTTTAAA TGTGTATCAGCATGTAGTAGGCATGTCCGCTGCCGAGTGTGCTTTGCACATGAGTCGTATTAGCACATGG CATATAGTTTATCTGACATTTGTATAGCAGTGACATTAGACCTCTTTGACAAAGCAATAATGTATATAGC ATGAGACAGATAAAATCACAACTATTTAAACTCCTTAAAACCATCGGTAAATGAAAAAATTTCCAATGGA AGTATTATTTAAGGATGGTTAGCCTTGGCTAACCTTCATGCCCAGTCAGCAACTTGGAAAAGTCTTTTCC TTTCTCCTGTGTGAAGCAAAAGTTGCTTTTCCTAACTTGCTATTGCTTTTCCTTGGCTGGAGCATAACAG CATAAAATCAGATAAAGGGCTCCCTTCAGTCCAGCCCCCGAGGCTGAGGAGGGCGTGAGGAGATGGCTTC TTATACAGGATTTGGCCAGCATCCTCCTCCGTGTCCCTGGTGTGCCATGGGTTGGCAGATCTACAGGAAC CCCACAAAGCCAACTTCTGCCTGGAGGTTAGCCCGAGGAGGAAGTAGGAGCCGGCAGAGCTGTCTGTCTC TCCACACACTCCAGTGCCCTGCTGCCTGGACCCTAAGGCCAGCCCACTCCAGAGGGCACAGGACAAGGGC ATTGTGGCTGCCATCATCACTAGCAAAGCACTGAGCACGTCCCACTTCTTACCCTTCTCTGGTTCCTGCT GTGGGAGCATGCGCAGTTCCTAGAGCTTAGTCCCTCTTGTTTTTAAAAAGAATGTTTTAAAAGTTACATG TTTTGTTCTGTTACTCAGATTGTAGCCAGAATAAAAGAAAATTAATTATAATGTATTCAAAAATGAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6408599 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6408599 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005698 same experiment
	EMAGE:31187 same embryo
	EMAGE:30172 same embryo
	EMAGE:31237 same embryo
	EMAGE:29624 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source