EMAGE mouse gene expression spatial database

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EMAGE:31187

Thbs1 ( MGI:98737)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005711_01	euxassay_005711_02	euxassay_005711_03	euxassay_005711_04	euxassay_005711_05

euxassay_005711_06	euxassay_005711_07	euxassay_005711_08	euxassay_005711_09	euxassay_005711_10

euxassay_005711_11	euxassay_005711_12	euxassay_005711_13	euxassay_005711_14	euxassay_005711_15

euxassay_005711_16	euxassay_005711_17	euxassay_005711_18	euxassay_005711_19	euxassay_005711_20

euxassay_005711_21	euxassay_005711_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

right lung

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20

sternum

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

mesenchyme

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

cranium

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

clavicle

strong

regional

strong expression: see section 07 14

axial skeleton

strong

regional

strong expression: see section 07 08 09 10 11

axial skeleton tail region

strong

regional

strong expression: see section 10 11 12 13 14

nose

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21

left lung

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09

lower leg mesenchyme

moderate

regional

moderate expression: see section 18 19 21 22

upper arm mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04

foot

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21

upper leg mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 15 16 17 18 19 20 21

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03

forearm mesenchyme

moderate

regional

moderate expression: see section 01

upper lip

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

naris

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 21 22

hand

moderate

regional

moderate expression: see section 01 02 03 04 05 06

lower lip

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8745
Entity Detected:	Thbs1, ( MGI:98737)
Sequence:	sense strand is shown >T8745 CGGACTTCTGCAGGCAATCGCGAAGCTGCTATCCAGTTCTGCCACGGTCTCTCCCGGCGCACCGGCAGTC TCAGCGTCTTCACCGGACTCAGCGTCCTTGTCCTTCACTTCACCTTTGCCACCTCTCCGGGTTACTGAGC CCCGGTGCACACAGGCTCCGTGTTGGGCACAAAGGCTCCACCATGGAGCTCCTGCGGGGACTAGGTGTCC TGTTCCTGTTGCATATGTGTGGAAGCAACCGCATTCCAGAGTCTGGGGGAGATAACGGTGTGTTTGACAT CTTTGAACTCATTGGAGGTGCACGAAGGGGCCCCGGTCGCCGACTGGTGAAGGGCCAAGATCTATCCAGC CCCGCCTTCCGCATTGAGAATGCCAACCTGATCCCCGCTGTGCCGGATGACAAGTTCCAAGACCTACTGG ACGCTGTGTGGGCCGACAAAGGCTTCATCTTCCTGGCTTCCTTGAGGCAGATGAAGAAGACCCGGGGCAC ACTCCTGGCTGTGGAACGGAAAGACAACACTGGCCAGATCTTCAGTGTGGTCTCCAACGGCAAAGCTGGC ACCCTGGACCTGAGCCTGAGCCTGCCAGGGAAGCAACAAGTGGTGTCAGTGGAGGAAGCTCTCCTGGCCA CTGGCCAGTGGAAGAGCATCACGCTGTTTGTTCAAGAGGACCGGGCTCAACTCTACATAGACTGTGATAA GATGGAGAGCGCGGAGCTGGATGTACCCATCCAGAGCATCTTCACCAGGGATCTGGCCAGCGTTGCCAGG CTCCGAGTTGCAAAGGGAGATGTCAATGACAATTTTCAGGGGGTGCTGCAGAATGTGAGGTTTGTCTTTG GAACCACCCCAGAAGACATTCTCAGGAACAAAGGCTGCTCCAGCTCAGCTACCAACGTCCTTCTTACCCT TGACAACAACGTGGTGAACGGTTCCAGCCCTGCTATCCGCACCAACTACATCGGCCACAAAACAAAGGAC CTCCAAGCTATCTGTGGCCTCTCCTGTGATGAACTATCCAGCATGGTCCTGGAACTGAAGGGCCTGCGCA CCATCGTGACCACTCTGCAGGACAGCATCCGAAAAGTGACGGAAGAGAACAGAGAGCTGGTCAGTGAGCT GAAGCGGCCTCCCCTCTGCTTTCACAATGGAGTCCAGTACAAGAACAACGAGGAGTGGACTGTAGACAGT TGCACAGAGTGTCACTGCCAGAACTCGGTTACCATCTGCAAAAAGGTGTCCTGTCCCATCATGCCCTGCT CCAACGCCACAGTTCCTGATGGTGAATGCTGCCCACGGTGCTGGCCCAGCGACTCTGCTGACGATGGCTG GTCTCCCTGGTCTGAGTGGACCTCCTGCTCTGCCACATGTGGCAATGGAATTCAGCAACGTGGTCGTTCC TGTGACAGCCTCAACAACAGATGCGAGGGCTCTTCGGTACAGACAAGGACCTGCCACATTCAGGAGTGTG ACAAAAGATTTAAACAGGATGGTGGCTGGAGTCACTGGTCTCCATGGTCGTCCTGTTCTGTGACCTGTGG TGACGGTGTGATCACAAGGATCCGGCTCTGCAACTCCCCCAGCCCCCAGATGAACGGGAAGCCCTGTGAA GGTGAAGCCCGGGAGACCAAAGCCTGCAAGAAAGACGCCTGCCCAATTAATGGAGGCTGGGGTCCCTGGT CACCATGGGACATCTGCTCTGTCACCTGTGGAGGAGGAGTGCAGAGACGCAGCCGACTCTGTAACAACCC CACACCCCAGTTTGGAGGCAAAGACTGTGTTGGCGATGTGACAGAAAATCAAGTTTGCAACAAGCAGGAC TGCCCAATTGATGGATGCCTGTCCAATCCCTGCTTTGCTGGTGCCAAGTGTACTAGCTACCCTGATGGTA GCTGGAAATGTGGTGCGTGTCCTCCTGGCTACAGTGGAAATGGCATCCAGTGCAAAGACGTCGATGAGTG CAAAGAAGTGCCTGATGCTTGCTTCAATCACAACGGAGAACATCGGTGCAAGAACACAGATCCTGGCTAC AACTGCCTGCCCTGCCCACCACGATTCACTGGCTCACAGCCCTTCGGCCGAGGTGTCGAACATGCCATGG CCAACAAACAGGTGTGCAAACCGCGAAACCCCTGCACGGACGGGACGCATGACTGCAACAAGAACGCTAA GTGCAACTACCTGGGTCACTACAGTGACCCCATGTACCGCTGTGAGTGCAAGCCCGGCTATGCAGGCAAT GGCATCATCTGCGGAGAGGACACAGACCTGGACGGCTGGCCTAATGAAAACCTGGTGTGTGTGGCCAACG CAACCTACCACTGCAAAAAGGACAACTGCCCCAACCTTCCCAACTCGGGGCAGGAAGACTATGACAAGGA CGGGATTGGCGATGCCTGCGATGATGACGATGACAACGACAAGATCCCCGATGACAGGGACAACTGTCCA TTCCATTACAACCCAGCCCAGTATGACTATGACAGAGATGATGTGGGAGACCGCTGTGACAACTGCCCCT ACAACCACAACCCTGACCAAGCAGACACAGACAAAAACGGGGAGGGCGATGCCTGTGCTGTGGACATCGA TGGAGATGGAATCCTCAATGAACGAGACAACTGCCAGTACGTTTACAACGTGGACCAGAGGGACACGGAC ATGGATGGGGTTGGAGATCAGTGTGACAACTGCCCCCTGGAACACAATCCAGACCAGCTGGACTCTGACT CAGACCTCATAGGGGACACTTGTGACAACAATCAGGACATCGATGAGGATGGCCATCAGAACAATCTGGA CAACTGTCCCTATGTGCCTAATGCCAACCAGGCCGACCATGATAAAGATGGCAAAGGAGATGCCTGTGAC CATGACGATGACAATGACGGCATCCCTGATGACAGAGACAACTGCAGGCTGGTGCCCAATCCTGACCAGA AGGACTCTGATGGTGATGGCCGAGGTGACGCCTGCAAAGACGACTTTGACCATGACAATGTGCCAGATAT TGATGACATCTGTCCTGAGAATTTTGACATCAGTGAAACCGATTTCCGACAATTCCAGATGATTCCTCTA GATCCCAAAGGAACCTCCCAAAATGACCCTAACTGGGTTGTCCGCCATCAGGGCAAAGAACTTGTCCAGA CTGTAAACTGTGACCCTGGACTTGCTGTAGGTTATGATGAGTTTAATGCTGTGGACTTCAGCGGTACCTT CTTCATCAACACCGAGAGAGATGATGACTACGCTGGCTTTGTTTTCGGCTACCAGTCCAGCAGCCGCTTC TACGTTGTGATGTGGAAACAAGTCACCCAGTCCTACTGGGACACCAACCCCACAAGGGCTCAGGGATACT CAGGCCTGTCTGTAAAGGTTGTGAACTCCACCACCGGCCCTGGCGAGCACCTGCGGAATGCACTGTGGCA CACAGGAAACACCCCTGGCCAGGTGCGCACCCTGTGGCATGACCCTCGCCACATCGGCTGGAAAGATTTC ACTGCATACAGATGGCGTCTCAGCCACAGGCCAAAGACCGGTTATATCAGAGTGGTGATGTATGAAGGAA AGAAAATCATGGCTGACTCGGGACCCATCTATGACAAAACCTACGCCGGCGGTAGACTAGGCCTGTTCGT CTTCTCTCAGGAAATGGTGTTCTTCTCAGACATGAAATACGAGTGTCGAGATTCCTAATCATCAGCTGCC AATCATAACCAGCGCTGGCAATGCACCTTCTAAAAACAAGGGCTAGAGAAACCCCCCACCCCTGCCGGGA TCGCCTTTCCTCGCCTTCCTTGCCTCTCTTCTTGCATAGTGTGGACTTGTAAAGCCTGAGACCTGCCTCA AGAAAATGCAGTTTTCAAACCCAGAGTCAGCACTCGGCCTTTAACGAATGAGAATGCATCTTCCAAGACC ATGAAGAGTTCCTTGGGTTTGCTTTTGGGAAAGCCAAAGCGCCTATTTACTTCCCACTAGGAAGGTGCCC GCTCCACTCTGCCTTACTCACAGAGCCAGAACTTCTTCGAGGCCACCTCTGAGCAGCACACACAGAAGCA TTTTCAGGCATGTCAAAGAAAGGAAAAAATGACTCACTAGAACTCACCGCCAAACAACCTCTGACATAGG TCCTGAGATGTGGGGAGGCAGGAGCCAAAGCTCTAGGGAGGGCATGTACCCAAGAGATGACTGTATGAAA ATGTGGAGGAGCTGTTCGGTACTAAATCATTTTCAGGGGACAGACAGACTTGCTGCATTTCTGCATGCTG CTGGTGAGAGCTGATTGACCCAATCTTCCACACAGGCACTTGAGCAAGCAGGGAAGGGAGGGAGATCATA GCTTCTGGACTTTCTCCCTTTGGGCACTTCTCACCTGCAGTGGCCAGGGTAGGGGTCAGAAGTGTGGGCC ATGCTGGCTGCCCTTGACTGGTCACGCTGAAACTTTTAGCTTATTCTGCATGTACAACATGCATATGTAT AAGGACAGCTAGAGAACCTTACCGTCTCAGTGAGCTCTAGCTGCCTCCGCAGGAGGGGCAGTGCGCCTTT ACTTTATGGTTAGAAAGGCACAAAATTTCTATCAACCTAACTAAAACATTCCTTTTCTTTCTTTCTTTCT TTCTTTCTTTCTTCCTTTTTCTCTCAGTTACCATGGATTTTTCCAAATCTCTTTTGGTTTTTGTTTAACA AATGCTTTAACAATGTAATATATTTATTTTGTTCCCCTATTCTGGAGGGTTCGTCGGAAGGATTGTTACT GAAACAGGAAGCGTGGGACTATCTGAATCATCTTCGTAGTGAGTCTTTATGACTGTAAGATTGTAAATAC AGATTATTTATTAACCCTGTTCTACCTGGAATTAGACTGCATATAGCAAATGTTTGCAAGCAGGTAGCTG AGGCGGATCAGCAAATCTCTCCAAAACCAGTGTGTGGAAAGGCAGCAGAGGCAGCTGCTTTTCATCTGGG GCTCACAGTGAATGATTTGGACTCCTTTGGCTTCCCCTTTGTTTTCTCTGTAGTTTCAAGTGGAATTAGG TGGCGGCCGCTTGCATTCACTCCTCCTTGTAATGGAAGCCAGGAGTACTCTAAGGCCCACACCACCCCTT GTGCCTATTTCCAGGGAGAAGAAAGAAAACACAATCACCTTTTCTTTATTTCATTTTTTCCCCAAAAGAG ACAAAGGTGGAACTTCTATACAAATATTACCTCATTTGTTGTGTGACTGAGTAAAGAATTTTGGATTAAG AAGAAAGAGTTTAAGTGTCAATAAACTTAAAACTACTGTTGTGTCTAAAAAGTCGGTGTTGTACATAGCA TAAAAATCCTTTGCCGAGGATGATCCCAAGAAAGAAACAGCTTTGTAATGTTAAGTACAACTTCTCAAAG TTCTGGTTTTGTTCTATCATCTTTTACACCGTTGCTTTTATTTTTATAAATTATTTTCTCGTTGCCATTG GAATAGAGATCTCAGACTATGTAGATATGCTATTTAAATAATTTATCAGGAAATACTGCCTGTAGAGTTA GTATTTCTGGTTTTTATATTTTGCACACTGAATTGAAGAAATGTTGGTTTTTCTTGTTTCGTTTTAGTTT GTTTCTTTGGTTTTGTTTTTGTTTTTGCTTTTTACTTCCCAGTTTTGACTCTTTGCCAATACCCTTTTTC CTAGGAACGTGCTTTTTTTTTTATTTTGTACACATTTTTATCCATTTTACATTCTAAAGCAGTGTAACTT GTATATTACTGTTTCTTATGTACAAGGAACAACAATAAATCATATGGAAGTTTATATTTATAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405710 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405710 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005711 same experiment
	EMAGE:30172 same embryo
	EMAGE:29864 same embryo
	EMAGE:31237 same embryo
	EMAGE:29624 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source