Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29885

Rps27a ( MGI:1925544)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885
euxassay_002175_01 euxassay_002175_02 euxassay_002175_03 euxassay_002175_04 euxassay_002175_05
EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885
euxassay_002175_06 euxassay_002175_07 euxassay_002175_08 euxassay_002175_09 euxassay_002175_10
EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885
euxassay_002175_11 euxassay_002175_12 euxassay_002175_13 euxassay_002175_14 euxassay_002175_15
EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885 EMAGE:29885
euxassay_002175_16 euxassay_002175_17 euxassay_002175_18 euxassay_002175_19 euxassay_002175_20
EMAGE:29885 EMAGE:29885
euxassay_002175_21 euxassay_002175_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6009
Entity Detected:Rps27a, ( MGI:1925544)
Sequence:sense strand is shown

>T6009
GCCATCGTGGGTGGAGCCGCCGCCACGATGCAGATCTTTGTGAAGACCCTTACGGGGAAAACCATCACGC
TCGAGGTTGAACCCTCGGACACTATAGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGGAATTCCTCC
TGATCAGCAGAGGCTGATCTTTGCTGGTAAGCAGCTGGAAGATGGCCGGACTTTGTCTGACTACAACATT
CAAAAGGAGTCCACCCTTCATCTGGTGTTGAGACTTCGGGGTGGTGCTAAGAAAAGGAAGAAGAAGTCTT
ACACCACTCCCAAGAAGAACAAGCATAAGAGGAAGAAGGTTAAGTTGGCTGTGCTGAAATACTATAAGGT
GGATGAAAATGGCAAAATTAGCCGACTTCGTCGAGAGTGTCCTTCTGATGAATGTGGTGCTGGAGTTTTC
ATGGGAAGCCACTTTGACAGGCATTACTGTGGCAAGTGTTGTCTGACTTACTGCTTCAACAAACCAGAAG
ACAAGTAGTTGTGTATGCGTTAATAAAAAGAAGGAACTCGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3487291 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487291 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002175 same experiment
 EMAGE:32186 same embryo
 EMAGE:32142 same embryo
 EMAGE:32135 same embryo
 EMAGE:29886 same embryo
 EMAGE:29889 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS