EMAGE mouse gene expression spatial database

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EMAGE:29889

Sepp1 selenoprotein P, plasma, 1 ( MGI:894288)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002178_01	euxassay_002178_02	euxassay_002178_03	euxassay_002178_04	euxassay_002178_05

euxassay_002178_06	euxassay_002178_07	euxassay_002178_08	euxassay_002178_09	euxassay_002178_10

euxassay_002178_11	euxassay_002178_12	euxassay_002178_13	euxassay_002178_14	euxassay_002178_15

euxassay_002178_16	euxassay_002178_17	euxassay_002178_18	euxassay_002178_19	euxassay_002178_20

euxassay_002178_21	euxassay_002178_22	euxassay_002178_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5993
Entity Detected:	Sepp1, selenoprotein P, plasma, 1 ( MGI:894288)
Sequence:	sense strand is shown >T5993 CCCACGCGTCCGTCTAACTAGAGCAAAGAGACAGGACGAAGCTAGTCCGAAGGGGTTGCGACAACCTCAG CAATGTGGAGAAGCCTAGGGCTTGCCCTGGCTCTCTGTCTCCTCCCCTATGGAGGAGCAGAGAGCCAAGG CCAAAGCTCTGCTTGTTACAAAGCCCCGGAGTGGTACATAGGAGATCAAAATCCAATGCTAAACTCAGAG GGCAAAGTGACAGTGGTTGCTCTTCTTCAAGCCAGCTGATACTTGTGTCTTCTGCAGGCATCCAGATTGG AAGACCTGCGCATAAAACTAGAAAGCCAAGGATATTTTAACATCTCTTACATTGTTGTTAACCATCAAGG ATCTCCTTCCCAATTAAAACACTCACATCTTAAAAAGCAGGTGTCAGAACACATCGCAGTGTACAGACAA GAAGAAGATGGCATAGATGTCTGGACTCTCTTAAATGGAAACAAAGATGACTTCCTCATCTATGACAGAT GTGGCCGTCTTGTGTATCACCTTGGTTTGCCTTACTCCTTCCTCACATTCCCATATGTTGAAGAAGCCAT TAAGATCGCTTACTGTGAGGAGAGGTGCGGAAACTGCAATCTCACGAGTCTTGAAGATGAAGACTTCTGT AAAAATGTGACCTCAGCTACTGCCAATAAAACTGCGGAGCCCTCAGAGGCTCATAGCCACCACAAACACC ACAACAAACATGGGCAGGAGCATCTTGGCAGCAGTAAGCCTTCAGAGAATCAGCAACCAGGGCCATCAGA GACGACTCTGCCTCCTTCAGGCTTGCACCACCACCACAGGCATAGGGGCCAGCACAGGCAGGGTCACTTA GAGAGCTGAGACACCACAGCAAGTGAAGGCTTGCACCTTTCACTTGCCCAGAGGAAGCTCTGACGAAGGG GGTGCATCAACCAGCTCCTGTGTAAGTTGTCTAAGGAGTCCGAGGCAGCCCCCAGCAGCTGCTGCTGTCA CTGCCGCCACCTCATATTTGAGAAGTCAGGGTCTGCAATTGCTTGACAGTGTGCGGAAAACCTCCCATCC TTATGTAGCTGACAGGGGCTTTTCGCGGAGGAGAAAGTCACTGAATCCTGTCAGTGTAGGTCACCTCCAG CTGCCTGACAAAATCAGCCCATGAACCCCATGGAAGCCAACCCCAACTGAAGCTGAGATAATCAGACCAG GAAGTGAAAATGACATTCAAACTAAATTATTTAAAACAAGGCATACCTCTCCCCAACTCAGTCTAAAGAC ACAATTTCATTTTGAGAATGTTTACAGCCCATTTAATTAATCAGTGAACTAAAAGTCATAGAAATTGGAT TTGTGCAAATGTAGAGAAATCTACCATATTGGCTTCCAAAATTTAAAAATTTTATGCCACAGAACATTTC ATCCAAATCAGATTTGTACAATAGGGCACCTGAAAAGTGACTGCAGCCTTTGGTTAATATGTCTTTCTTT TTCCTTTTTCCAGTGTTCTAGTTACATTAATGAGAACAGAAACATAAACTATGACCTAGGGGTTTCTGTT GGATAGCTTGTAATTAAGAACGGAGAAAGAACAACAAAGACATATTTTCCAGTTTTTTTTTTCTTTACTT AAACTCTGAAAACAACAGAAACTTTGTCTTCCTACTCTTACATTCTAAACCGATGAAATCTTTAACAGAT TACACTTTAAATATCTACTCATCATTTTCTCTCTCAGAGTCCTAGCTTGAGTTGCACTGCATGTATCTGT GCATCTTGTTCTCTTCATTTAATGCTGTACTGTTCTGCTGAGCTCTGAGGGACTATCTTGAGAGATGTAA TGGAAGGAAAGCGTGGTGTTAATCTGCGTACTGCTTAAGACAGTATTTCCATAATCAATGATGGTTTCAT AGAGAAACTAAGTCCTATGAACCTGACCTCTTTTATGGCTAATACGACTAAGCAAGAATGGAGTACAGAA TTAAGTGGCTACAGTACACACTTATCAAAATAAATGCAATTTTAAAACCTTTAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3486761 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3486761 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002178 same experiment
	EMAGE:32186 same embryo
	EMAGE:32142 same embryo
	EMAGE:29885 same embryo
	EMAGE:32135 same embryo
	EMAGE:29886 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source