EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30239

Plekho1 ( MGI:1914470)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007900_01	euxassay_007900_02	euxassay_007900_03	euxassay_007900_04	euxassay_007900_05

euxassay_007900_06	euxassay_007900_07	euxassay_007900_08	euxassay_007900_09	euxassay_007900_10

euxassay_007900_11	euxassay_007900_12	euxassay_007900_13	euxassay_007900_14	euxassay_007900_15

euxassay_007900_16	euxassay_007900_17	euxassay_007900_18	euxassay_007900_19	euxassay_007900_20

euxassay_007900_21	euxassay_007900_22	euxassay_007900_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35589
Entity Detected:	Plekho1, ( MGI:1914470)
Sequence:	sense strand is shown >T35589 CCATTACCAGAGCTAAAAACCGTATCTTGGATGAGGTCACCGTTGAGGAGGACAGCTATCTTGCCCACCC TACTCGAGACAGAGCAAAAATCCAACACTCCCGCCGTCCTCCAACCCGGGGACACCTCATGGCTGTGGCT TCGACCTCTACCTCAGATGGGATGCTAACATTAGACCTGATCCAAGAGGAAGACCCTTCCCCTGAGGAGC CAGCCTCTTGTGCTGAGAGCTTCCGGGTCGATCTGGACAAGTCTGTGGCCCAGCTGGCTGGCAGTCGACG GAGAGCGGACTCAGACAGGATCCAGCCCTCTTCGCAGCGGGCAAGCAGCCTTTCTCGGCCTTGGGAAAAA CCAGACAAGGGAGCCCCCTACACCCCACAAGCACTGAAGAAGTTCCCCAGTACAGAGAAGAGCCGATGTG CCTCCCTGGAGGAGATCCTATCCCAGAGGGACACTGCCCCAGCCCGCCCCCTTCACCTTCAAGCTGAGGA ATCCCTACCCCCTGTCCCTGCCCAGCCAGGGCAGCTGTCCCGGATCCAGGACCTGGTAGCAAGGAAACTG GAGAAGACTCAGGAGCTGCTGGCAGAGGTTCAGGGACTGGGTGACGGCAAGCGGAAGGCCAAGGACCCCC CACAGTCTCCACCCGACTCGGAGTCGGAGCAGCTGCTGCTGGAGACAGAGAGGCTGCTGGGAGAGGCTTC CTCCAACTGGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91186. Forward Primer - name:091186_F_cDNA_2810052M02Rik, sequence:CCATTACCAGAGCTAAAAACCG; Reverse Primer - name:091186_N_SP6_cDNA_2810052M02Rik, sequence:CTCCAGTTGGAGGAAGCCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007900 same experiment
	EMAGE:31637 same embryo
	EMAGE:30241 same embryo
	EMAGE:30173 same embryo
	EMAGE:29621 same embryo
	EMAGE:30238 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source