EMAGE mouse gene expression spatial database

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EMAGE:30288

AA415817 ( MGI:1920909)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004821_01	euxassay_004821_02	euxassay_004821_03	euxassay_004821_04	euxassay_004821_05

euxassay_004821_06	euxassay_004821_07	euxassay_004821_08	euxassay_004821_09	euxassay_004821_10

euxassay_004821_11	euxassay_004821_12	euxassay_004821_13	euxassay_004821_14	euxassay_004821_15

euxassay_004821_16	euxassay_004821_17	euxassay_004821_18	euxassay_004821_19	euxassay_004821_20

euxassay_004821_21	euxassay_004821_22	euxassay_004821_23	euxassay_004821_24	euxassay_004821_25

euxassay_004821_26	euxassay_004821_27	euxassay_004821_28

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6516
Entity Detected:	AA415817, ( MGI:1920909)
Sequence:	sense strand is shown >T6516 CGCGGGCCCAGCACCGGACGCCTCAGCCATGGACGCGTCCCTGGAGAAGATAGCAGACCCTACATTAGCT GAAATGGGAAAAAACTTGAAAGAGGCGATGCGGATGCTGGAGAAGAGCCCGAGAAGAACAGAGGAGGAAA ATGGAAAGAAGCCAGTATCGGAGGATATTCCAGGGCCACTCCAAGGCAGTGGACAAGATATGGTGAGCAT CCTCCAGTTAGTTCAAAATCTGATGCATGGAGATGAAGACGAGGAGCCCCAGAGCACCCGAATCCAAAAC ATTGGAGAGCAAGGTCACATGGCTTTGTTGGGTCATAGCCTCGGTGCCTATATTTCAACTCTGGATAAAG AGAAGCTGAGAAAGCTCACAACCAGGATACTTTCAGACACTACCTTATGGCTACGCCGGATTTTCAGGTA CGAAAATGGATGTGCATATTTCCACGAGGAAGAGAGGGAAGGACTTGCTAAGATCTGTAGGCTTGCCATT CATTCTCGCTATGAAGACTTTGTTGTAGATGGATTCAACGTGTTATATAACAAAAAGCCTGTCATATATC TTAGTGCTGCCGCTAGACCTGGCCTGGGCCAATACCTTTGTAATCAGCTTGGCTTACCCTTCCCCTGCCT GTGCCGTGTGCCCTGTAACACTATGTTTGGATCCCAGCATCAGATGGACGTTGCCTTTCTCGAGAAACTG ATCAAAGATGACATAGAAAGAGGAAGACTGCCCTTGCTGCTTGTTGCAAATGCTGGAACTGCAGCAGTTG GACACACAGACAAGATTGGACGTTTGAAGGAACTCTGTGAGCAGTACGGCATTTGGCTTCATGTGGAAGG TGTGAATCTGGCAACATTGGCTCTAGGTTATGTCTCCTCATCTGTGCTGGCTGCAACCAAGTGCGATAGC ATGACACTAACTCCTGGCCTATGGCTAGGTTTGCCAGCTGTTCCTGCAGTGACACTCTACAAACATGACG ATCCTGCCTTGACACTAGTTGCTGGTCTTACATCAAATAAGCCTGCAGACAAACTCCGAGCCCTACCACT CTGGTTGTCTCTACAGTACTTGGGACTTGATGGCATTGTGGAGAAAATAAAGCATGCTTGTCATCTGAGT CAACGGTTGCAAGAAAGTTTGAAGAAAGTGGACCACATCAAAATCTTGGTGGAAGATGAGCTCAGTTCTC CAGTAGTGGTGTTCAGGTTTTTCCAGGAATTACCAGCCTCAGATTCAGCGTTTAAAGCTGTTCCTGTATC CAACATAGCACCTGCAGCAGTTGGCCGGGAAAGACACTCCTGTGATGCACTGAACCGCTGGTTGGGTGAA CAGTTGAAACAGTTGGTGCCTCAGTGTGGCCTCACTGTCATAGACCTGGAGGTAGATGGCACATGTGTTC GGTTCAGCCCTCTGATGACAGCAGAAGGGTTAGGAACTCGAGGAGAGGATGTCGATCAGCTCATTACCTG CATCCAAAGCAAACTACCAGTCCTGACCTGCACACTACAGCTGCGGGAAGAATTCAAGCAGGAGGTAGAG GGGACAGCAGGGCTCCTGTATGTTGATGACCCCAACTGGCCTGGAATCGGGGTTGTCAGGTATGAGCATG CGAATGATGATGACACCAGTTTGAAATCTGATCCAGAGGGGGAAAAAATACACACTGGACTTCTGAAAAA GTTAAATGAACTGGAATCTGACCTCACATTTAAAATAGGCCCTGAGTACAAAAGCATGAAGAGCTGCATT TACATTGGCATGGCAAGTGATGATGTGGACGTTTCTGAGCTAGTGGAGACCATTGCAGTCACAGCCCGGG AAATTGAGGAGAACTCAAGGCTTCTAGAGAACATGACAGAAGTTGTTCGGAAGGGAATTCAGGAGGCACA GGTTCAGCTACAGAAGGCAAATGAGGAGCGGCTTCTGGAAGAGGGAGTGTTGCGGCAAATCCCTGTAGTA GGATCCGTGCTGAATTGGTTTTCTCCAGTCCAGGCTTCACAGAAGGGAAGAAGTTTTAACTTAACAGCAG GCTCTCTGGAGTCCACAGAATACACCTATGTCCACAAAGTACAAGGTACTGGGGTGACACCACCTCCCAC ACCCCTAGGCACTCGAAGCAAACAGAGGCTTCCAGGTCAGAAGCCTTTTAAAAGGTCCCTGAGAGGCTCG GATGCTGTGAGTGAGACCAGCTCAGTCAGCCACATTGAAGACCTGGAAAAGGTGGAACAGCTGTCCAGTG GGCTAGAGCATGACAACCTAGAGGCCCACAGCCCTGAGCAGCCTCCCAGGGCTGCTGATCTCACGGCCAG ACAGACTGAGGCCCTGCAGAACCAGGCCCAGCATCAGGAAGATGACCATTCACAGGTAGAGGAACTGGAG CGCTTAAGATAAGCTCAGTAGTGCTGGGCTGGAGGCTGTACTAACTGTCGTTTCAGGGAAGGTGAAGTTC TATGGAAGACGTGAACTATGCCACATGTTAATATGATAAAAACTTTTGTTGTTTGTGCTAAACTGGGAGT TTTGCACAGACACATGTCAGAAAAGCGGGCTTTCTAGGGGGCAATTAATTATTTTATCTGTAAGTAGAAC AAATGACAATTGACTATTTTGGCCTGTCCAACATAGGCATACCCTTTCTTCCTGTACCACACATTTTGTA CTGAGAACCGTGGCCTTCAAGCACCTGCCACTTGAAACACTGCTCTCATGGCTCTGAAGCTAATCGTGAT GAAGCTGCATACAGTGACTCTCCAGGTTCTCCTCGGTCTGTCGTAGTAGCTCTGTAGGTCAGCCGAGTTG TGGTAGGTGCCTGCCTCACTGGTTGGCATCAGGAGCTCCTCTGCCTCTGTCCCCACTGCTGCTTCTCCGG GAGCCTAAGTAAGGACTGTCCACATGAGCTGTGGCTAGGACTGCCCCTTGCTGCACCTTTTCTCCTTACA CTCTGTAAACAAATGTAAGGACTCACCTGTTAATAGAGTAATTAACTCTTAAGATCTCTTCCTGACCATA TTTAAGTGTATTTTTAAACACTGGTAATGCTTTTAAGTTGGTGCCCAGTGTGCACTGTGCTTGTGTTTAT TTGGGACAGAAAGCTGCCATGACGAGTCAGTGTCGTCTGCTGTGCAGCACGGTAAGGCTGAAGCAGACAA GCCCTCGGCCTGCTTTCCATGGTGCGGTTGTAGCTTCTCTTCTACTTGAAGCTCACCTGGGCCCAGCTCA GGAAAGAGCCAGAGAAGTCGCCTCTTCCAGGCTAACAAGCCAGAGCTTGCTGCCTCATTCCTGTCCATTT CTATTTCCCCCAAGTACTGTATCACTTGTGGTACCCTAAGATGCCCCACCCCGTGCCTTTCTAGTCCCTA AAAGTCAAACAGTGGAAGATGCTAAGATGGTGTTCCTGCAGACTGCAGTGTTAGAGCCTTAGTTACACCA CATGAAGCCTATAATTACACTGAGTTCACCGGGGTGATTGCTGACAGCTGCCCCTTGTCAACATGCAGTC TTTCCCTTTTTGGGGTGTAAATGATTAAATACAGTCTTCCCTGTTTATTTGGTGCAATGAAGTATGGCAG GTGACATGGGGAAGGGGTAAATTATTACCTTTAACAAGATTGATTTTTAAATGTTTTTGTACATTTGGAA TTGTTACCTTGGTTTTGCTGCAACAGAACTTACCTTGAGACTATCTGATGTTGGCTTAAATAAAGGCTCT TGAAATTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590316 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590316 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004821 same experiment
	EMAGE:29649 same embryo
	EMAGE:29650 same embryo
	EMAGE:31685 same embryo
	EMAGE:31298 same embryo
	EMAGE:31228 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source