EMAGE mouse gene expression spatial database

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EMAGE:31228

Mthfd1 methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase ( MGI:1342005)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004845_01	euxassay_004845_02	euxassay_004845_03	euxassay_004845_04	euxassay_004845_05

euxassay_004845_06	euxassay_004845_07	euxassay_004845_08	euxassay_004845_09	euxassay_004845_10

euxassay_004845_11	euxassay_004845_12	euxassay_004845_13	euxassay_004845_14	euxassay_004845_15

euxassay_004845_16	euxassay_004845_17	euxassay_004845_18	euxassay_004845_19	euxassay_004845_20

euxassay_004845_21	euxassay_004845_22	euxassay_004845_23	euxassay_004845_24	euxassay_004845_25

euxassay_004845_26	euxassay_004845_27	euxassay_004845_28

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midgut loop lumen

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21 22 23 24

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 17 18

liver right lobe

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27

right lung

strong

regional

strong expression: see section 13 14 15 moderate expression: see section 16 17 18 19 20 21 22 23 not examined expression: see section 09 10 11 12

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

left lung

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

axial musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 14 15 16 17 18 19 20

esophagus

moderate

regional

moderate expression: see section 10 11

pancreas

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15

lower jaw molar

moderate

regional

moderate expression: see section 07 09 17 18 19 20

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09 15 16 17 18 19

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 17 18 19 20

thymus primordium

strong

homogeneous

strong expression: see section 09 10 11 12 13 14

stomach fundus

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 weak expression: see section 08 09 10

vibrissa

moderate

regional

moderate expression: see section 07 08 09 weak expression: see section 20 21 22

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22 23 24

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 12 13 14

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12 14 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 13 15 16 17

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6517
Entity Detected:	Mthfd1, methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase ( MGI:1342005)
Sequence:	sense strand is shown >T6517 TCCCTGAGCACGTGGGTTGTGCTGTCCTTCGCGCGCGGAGCGACTGGAACCTGATTTTTGCTGGTGTTCA CCGTGGGTGGCAGACTAGCAAAGGCCATGGCACCAGCGGGAATCCTGAACGGGAAACTGGTTTCTGCGCA AATCAGGGATCGACTGAAGAACCAGGTAACTCGGATGCAGGAGCAGGTACCTGGCTTCACCCCTGGCCTG GCGATCCTGCAGGTTGGCGACAGAGATGATTCTAATCTTTATATAAATGTGAAGCTGAAGGCTGCTGAGG AGATTGGGATCAAAGCCACTCACATTAAATTACCAAGAACATCCACAGAGTCCGAGGTATTAAAATATGT CATCTCCCTGAATGAGGATGCCTCTGTGCACGGATTCATAGTGCAGCTGCCTTTAGATTCAGAGAATTCC ATTAACACTGAGGCAGTCATCAATGCCATTGCACCCGAGAAGGATGTGGATGGGTTGACGAGCGTCAGTG CTGGGAAGCTCGCCAGAGGTGATCTAAATGACTGCTTCATCCCATGCACACCCAAGGGATGCTTGGAACT CATCAAAGAGGCAGGGGTGCAGATTGCCGGAAGGCACGCGGTGGTGGTTGGTCGAAGTAAGATAGTTGGT GCACCCATGCATGACCTCCTTCTGTGGAACAATGCCACGGTGACCACCTGCCACTCCAAGACTGCCAATC TGGACAAGGAGGTAAATAAAGGAGACATTCTGGTGGTTGCCACAGGCCAGCCTGAGATGGTGAAGGGGGA GTGGATCAAGCCTGGGGCTGTGGTCATAGACTGTGGGATCAATTATGTTCCAGATGATACAAAACCAAAT GGAAGGAAAGTCGTGGGTGATGTGGCCTACGATGAGGCCAAGGAGAGGGCGAGCTTTATCACGCCTGTCC CTGGTGGCGTGGGGCCCATGACCGTGGCAATGCTGATGCAGAGCACAGTAGAGAGCGCACAGCGCTTCCT ACAGAAATTTAAGCCAGGGAAGTGGACAATTCAGTATAACAAGCTGAACCTCAAGACTCCTGTCCCAAGT GACATTGCTATATCACGATCTTGCAAACCAAAGCTCATCGGGAACTTGGCCCGAGAAATCGGGCTGCTCA CCGAAGAGGTGGAGTTGTACGGGGAAACGAAGGCTAAAGTCTTGCTGTCAGCATTAGATCGCCTGAAGTA TCAGCCAGATGGGAAATATGTCGTGGTGACTGGAATTACTCCAACACCGCTGGGAGAAGGGAAGAGTACC ACCACGATCGGGCTGGTGCAGGCCCTCGGCGCCCACCTGCGACAGAACGTCTTTGCGTGTGTGCGACAGC CTTCGCAGGGCCCCACCTTTGGGATAAAAGGTGGCGCTGCAGGAGGCGGCTATTCCCAGGTCATTCCAAT GGAAGAGTTTAACCTCCACCTCACTGGTGACATCCACGCCATCACTGCTGCTAACAACCTCGTGGCTGCT GCCATTGATGCTCGGATATTTCATGAGCTGACCCAGACAGACAAGGCTCTCTTTAATCGTCTGGTACCTT CAGTAAACGGAATAAGAAAATTCTCTGACATCCAAATCCGCAGGTTACGGAGGCTAGGCATTGAGAAGAC TGACCCCACCACGCTGACAGATGACGAGATAAACAGATTTGCAAGACTGGATATTGACCCAGAAACCATA ACATGGCAGAGAGTGTTGGACACCAACGATAGATTCCTGAGGAAGATCACAATTGGACAGTCTCCAACAG AGAAAGGCCACACACGCACGGCCCAGTTTGATATCTCTGTGGCCAGTGAGATCATGGCAGTCCTGGCCCT CACGAGCTCTCTGGAAGACATGAGAGAGAGACTGGGCAGAATGGTGGTGGCGTCCAGTAAGAAAGGGGAG CCTATCAGTTGCGAAGACCTGGGCGTGAGTGGGACGCTGACGGTGCTGATGAAGGATGCAATCAAGCCCA ACCTCATGCAGACCTTAGAGGGCACGCCAGTGTTTGTCCATGCCGGGCCTTTCGCCAACATTGCACACGG GAATTCCTCCATCATTGCAGACCGGATTGCACTCAAGCTGGTAGGCCCTGAGGGCTTCGTAGTGACGGAA GCTGGATTCGGAGCAGACATAGGAATGGAAAAGTTCTTCAACATCAAGTGCCGGTATTCTGGTCTCCAGC CACACGTGGTGGTTCTGGTTGCCACTGTCAGGGCCCTTAAGATGCACGGGGGTGGCCCCACGGTCACCGC TGGACTACCTCTTCCCAAGGCTTACACAGAAGAGGACCTGGACCTGGTTGAAAAGGGCTTCAGTAACTTG AGGAAACAAATCGAAAATGCTAGAATGTTTGGAGTCCCTGTCGTCGTGGCTGTGAACGTGTTCAAGACAG ATACAGATGCTGAGCTGGACCTTGTCAGCCGCCTCTCCAGAGAACACGGGGCTTTTGATGCTGTCAAGTG CACCCACTGGGCAGAAGGGGGCCAGGGGGCCTTGGCCCTGGCTCAGGCTGTCCAGAGAGCGTCACAGGCC CCCAGCAGCTTCCAGCTCCTCTATGACCTCAAGCTCTCAATTGAGGATAAAATCAGGATTATTGCACAGA GGATCTACGGGGCCGATGACATCGAATTGCTCCCGGAAGCACAGAACAAAGCAGAAATTTACACAAAGCA GGGCTTTGGGAATCTACCCATCTGCATGGCCAAAACACACTTGTCCTTGTCTCACAACCCAGAGCAAAAA GGGGTGCCTACTGGTTTCGTTCTGCCCATCCGGGACATCCGTGCCAGCGTTGGGGCAGGTTTCCTGTATC CTTTAGTAGGAACGATGAGCACAATGCCTGGACTCCCTACGAGGCCCTGTTTTTATGATATTGATTTGGA TCCTGAAACGGAACAAGTGAATGGATTGTTTTAAGCAGATCTTCCATCTCCAAGAGGCCACTCTGTCCGG CCAGTGTCTGTTCAGGCCCACTGAAGAAGTGTGCAGAAGTCTTGGAAGTCTGTCCCCGCCCTGAAGAGCT TCAGAAATAGTGGGAGTTTCCCTAAAGCCTTTCATAGCCTTAATTCACGTCATGTATAAATTAACATAAA TCATGTCTATTTACATAGTGAAGGTCCAGAATAAATGAAATAATTTTGCTAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590449 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590449 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004845 same experiment
	EMAGE:29649 same embryo
	EMAGE:29650 same embryo
	EMAGE:31685 same embryo
	EMAGE:30288 same embryo
	EMAGE:31298 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source