EMAGE mouse gene expression spatial database

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EMAGE:30642

D2Ertd485e ( MGI:1196405)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004984_01	euxassay_004984_02	euxassay_004984_03	euxassay_004984_04	euxassay_004984_05

euxassay_004984_06	euxassay_004984_07	euxassay_004984_08	euxassay_004984_09	euxassay_004984_10

euxassay_004984_11	euxassay_004984_12	euxassay_004984_13	euxassay_004984_14	euxassay_004984_15

euxassay_004984_16	euxassay_004984_17	euxassay_004984_18	euxassay_004984_19	euxassay_004984_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8045
Entity Detected:	D2Ertd485e, ( MGI:1196405)
Sequence:	sense strand is shown >T8045 AAGAGGTAATCCCTGAGGGATAAGCGGGAAAGACACCACCTATTGCGCAGTCGGGACCATCGCCGGAGCC CGAAGACACTTTTCTGTCGTCACAGTGAAGGTTTACATGTGGCACCCGTAAAAGATGAAGCTGAGAACAC GGAAGGCTTCTCAGCAGTCCAGTCCAATCCAGACACAGCGCACTGCCAGAGCAAAGAGGAAATACTCAGA GGTTGATGATAGCTTGCCTTCAGGAGGAGAAAAACCATCAAAGAATGAAACCGGCTTATTGTCTTCAATT AAAAAGTTCATTAAAGGAAGCACACCCAAGGAAGAGAGAGAAAATCCTTCGAAGCGGAGTAGAATTGAAC GTGATATAGATAATAATTTAATCACATCAACACCAAGAACCGGAGAAAAGCCTGACAAACAGTTGTCTCG AGTAAGACGGAAAAATCCAGTCAATGGAGAAGCTGGTAGTTATGAAATGACAAATCAACATATAAAGCAA AATGGAAAATTAGAAGATAACCCTTGCTCTGGTAGTCCTCCGAGGACTACATTGTTGGGGACCATATTTT CGCCTGTCTTCAACTTTTTTTCACCAGCAAATAAAAATGGAACGTCAGGCTCAGATTCCCCAGGACAGGC TGTGGAAGCTGAGGAGATAGTGAAACAACTTGACATGGAACAGGTGGATGAAATTACTACCAGCACCACG TCAGCTAATGGAGCGGCTTACTCAAATCAAGCAGTTCAAGTGAGGCCATCACTAAATAATGGTTTAGAAG AAGCAGAAGAAACAGTTACCCGGGATATCCCACCCCTTACAGCACCAGTAACTCCAGAGAGTGGGTACTC GTCAGCCCATGCAGAGGCCACCTATGAAGAAGACTGGGAAGTATTTGACCCCTATTATTTCATCAAACAT GTTCCGCCTCTGACAGAAGAACAGCTAAATAGGAAACCTGCTCTTCCTTTGAAAACAAGAAGCACACCAG AGTTCTCCCTGGTCTTAGATCTGGATGAGACACTAGTGCACTGCAGTCTGAATGAGCTAGAAGATGCAGC GCTCACTTTTCCAGTCCTTTTCCAAGATGTCATTTATCAGGTTTATGTGAGATTAAGACCATTCTTCAGG GAATTCCTGGAACGAATGTCTCAGATGTATGAGATCATTCTTTTTACTGCTTCTAAGAAGGTGTATGCAG ACAAGTTACTGAACATACTAGACCCTAAAAAGCAACTGGTCAGGCACCGGCTTTTCCGTGAACATTGTGT TTGTGTACAAGGAAACTATATAAAGGACTTGAATATCCTTGGGAGAGATCTTTCCAAAACTATAATAATT GACAACTCACCACAAGCCTTTGCCTATCAGCTTTCTAATGGAATCCCTATAGAAAGCTGGTTTATGGATA AAAATGACAATGAACTCCTAAAATTGATTCCATTTCTGGAGAAGCTTGTAGAACTGAATGAAGATGTCCG GCCTCACATCAGAGACAGATTTCGATTGCATGATTTGCTGCCCCCAGATTAAGTTCAGAGACTTGTCATA TCACTGAAGGGGGGGAGAGAATGCAGGACCCTTTTGGACTAAGACAAAAACATTGCCATTACTGTTGAAA TTTTGCATTTTTGTACCCCGTCTTGCTTTCTTATCTTTGGTGCCCAATAATAATCAAGGGTTACAGAAAG AGACTCTCGGATTGAACACATACAGTGGGTAGGCTAAAACAGAAGCGTCTGTAGAGTAGACAGCTCCAGT GAGACTCTATGTATAGGACATCTGCAGCTCATAACGAATTCTGTTTCTGCCACCAGCAGTTTGACCTGTA GTTACACAGGATTTTATGATAATAACAGAGATGAAAGTGGACTTTCATTCTCTCTCTGTTTGGTGCTCTC AGTGGGTTTGTAGCCACTCTTCTGTTACTTCACTATTCTCATTTCAACAGGAATGGCCAGTAAAAGTTGT TTTATTTTTCCTGTTAAGGTTTATAACCTTTTTACATTTCTGACCTGTATTAGATTAGAATCTCATGATG CATTCCTTTTAGTTAAGGCGCTTCATAGTTTTCTGGAAATAGTCAATTTTTAGTTTTTTTTTTAATTATA CATTTGAATGGCCGTTTTCCTGCTTTGTCTGCCTGCATATTGTATATTTGTTTAAAAATATTCTCTACTT TTAGTCCATGTTAAGTTTCATTTAAAAGATGCATTTATATTTTGTTCTGTAATATTCTACTGTAGGTGAA ATCTTCAAAAATCAAGAGACTGGGTAGTTAAGAATATATGAATGACTAATATTCAAATGATTTGAATGAA CCATTGTGATGACATGTATTCCAGATGGTAATATCTTGCAATGGCACACGTTTAATGGATAAAGGTATAC CTCAGACTTCACTGTGCTCACTAGCCTTTGAGGAGAACGAGTTCAGGCACCTGTTGGGCTTGATTCGGTT ACTGCTGCCTTTGGGTTTTCTCCAGGAAGGAAGTATTCAGCACAGTGACTCCGTATTTTTACTTTGCATG GGCTGGTAGTACCTCTGTTATGCTCCCAGTTACAATCAGTTTAAAGCTCTGTGTAACAGCTGTGGTACCT AACGGTGGCTATGCATAATCCTGTGGCACAGTACACTCCCGGGCCACCAGTGCAGTCAATGTGCTCATAG TGGTTTTCTATGCTATATGAAAATAAGTCGTCACGCCTTGGTTCTCTAATGCAGCTACCACAAGAGGTTG ATATTTTTATAGTGGCGTGTAATCTCCTTTTCAGGAGGCTTTTTATGGAAGGTAGAATTTGTAAAAGTCA GTATGCTTTGCCTCTCAACTGCATTAACATGCCGCAGGCTCAGACTGTTTTTGTGTAAAGGATGTCAAAG AACGGCACTTTTTCTAAAGAGAAGTTTGATATTTTGTATGCTTGTTAAGAAAGTACAGTATTGGAAATTA AAGGTGGACAATTGATAATTGAGGAGTATGTCAGTTAATTTTTTATGTATATTACCTGTTTACTTGTACA ACTTATTGTACAAATTACATGCAGCTTCATTTTCAAGTGAATCCTTAAAATAAGGAAATCTTTTTAGGAA AACATTTAATTTTTGTATTTTTGATTTTAAAGGCATGAGTTGTCAGTTTTCAGTGCATTAATGAAGATTT TAACTTTTCATCAGGTTGAGTGTTTTCTTACTATATTATCTGTTGTGTATGTAGTTAGCATATTGTGTCA CTGAACTGTTTAAAAATCTAGCATGTAGAGCAAGCCTGTTTTGTGGAAAATCCTTATTCATTAAAAAAAA TAATCATGGCAGATTTTCTGCAAAAAAAAAAAAAGCAAAAGCATTTGCAATTCAGATTTTTTTTATTTAA AAAAAAAAGGTATTTTGCTTGCAGGAACAATACTACAGATTCATTTTAATGAGAATCTTCGAAATGTATT TATCTTTAGGGCACCTGGGCATCTTTATGCTGTTTGTCTTATGTCTTTCTTGAAATAAAATGTAAATATG TTACCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30049400 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30049400 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004984 same experiment
	EMAGE:31030 same embryo
	EMAGE:31965 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source