EMAGE mouse gene expression spatial database

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EMAGE:31965

Spag5 ( MGI:1927470)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004999_01	euxassay_004999_02	euxassay_004999_03	euxassay_004999_04	euxassay_004999_05

euxassay_004999_06	euxassay_004999_07	euxassay_004999_08	euxassay_004999_09	euxassay_004999_10

euxassay_004999_11	euxassay_004999_12	euxassay_004999_13	euxassay_004999_14	euxassay_004999_15

euxassay_004999_16	euxassay_004999_17	euxassay_004999_18	euxassay_004999_19	euxassay_004999_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8044
Entity Detected:	Spag5, ( MGI:1927470)
Sequence:	sense strand is shown >T8044 AAGAGGTAATCCGGTTAAGTGATCGAGTTGAAAATGTGGAGGGTGAAAACACTGAATCTCGGTTTGTCGC CTTCGCCCCAAAAGGGGAAGCCAGCTATGAGTACTCCTCTCCGAGAGCTTAAACTACAGCCCGAGGCCCT CGCCGATTCAGGGAAAGGTCCCTCTATGATCTCCGCGCTGACCCCATACCTGTGCAGGCTGGAGCTGAAG GAAAGATGCAACAACTCATCTCCAGTGGATTTTATCAATACTGAGAACAACTTCCTTTCAGAACAGTTCA GCCATCCTTCAACGCACATAGAAGCTTGCCAGCGTGAATCCGATCCAACTCCTGAAAGCAACTCTCTTTT CCATACTTTGGAGGAAGCAATAGAAACAGTGGATGACTTTGTCGTGGACCCAAGAGATGATAGTATAGTG GAGTCCATGGTTCTTCTGCCCTTTTCATTAGGGCAGCAGCAAGACCTGATGCTCCAGGCTCACTTAGATA CCACAGCAGAGAGAACTAAAAGCTCTCTAAATGAATCTTTGGGGCTAGAAGATCTGGTGGGGAAGGAGGT GGCACCTTGTGTGGAAGACAGCCTAACAGAAATTGTTGCTATTAGGCCCGAGCAACCTACATTTCAGGAC CCTCCGTTAGGACCCAGTGACACTGAAGATGCACCTGTGGACTTAGTTCCTTCTGAAAATGTCCTGAATT TCTCTCTGGCTCGTCTTTCTCCTTCAGCTGTCCTGGCCCAGGATTTCTCTGTCGATCATGTCGATCCAGG GGAGGAAACCGTAGAGAACAGAGTCCTACAGGAAATGGAAACGAGCTTCCCCACATTCCCTGAGGAGGCC GAATTGGGAGACCAAGCACCTGCTGCAAATGCAGAAGCCGTCTCACCCTTGTACCTGACATCAAGTCTAG TGGAGATGGGGCCCCGGGAAGCCCCAGGCCCAACAGTAGAAGACGCCAGTAGGATTCCTGGCCTTGAGTC AGAGACTTGGATGTCCCCATTGGCCTGGCTAGAAAAGGGTGTGAATACATCGGTCATGCTACAAAACCTC CGCCAGAGCTTATCCTTTTCTTCTGTGCTTCAGGATGCTGCCGTTGGCAACACACCCCTCGCCACGTGTT CTGTGGGCACTTCCTTTACTCCTCCAGCACCACTGGAGGTAGGCACCAAGGACAGTACTTCAGAGACAGA GCGCCTCCTCTTGGGCTGCCGTCCTCCAGATCTGGCTACCTTGTCCCGACACGACTTGGAAGAGAACCTG TTGAACTCTCTTGTCTTGCTGGAAGTTCTTTCCCACCAGCTACAAGCCTGGAAGAGCCAGTTGACTGTCC CTCACCGGGAAGCTCGAGACAGTAGTACACAGACTGACAGCTCTCCTTGTGGGGTCACTAAGACACCTAA ACATCTTCAGGACAGCAAGGAGATTAGACAGGCTCTACTGCAAGCCAGGAATGTCATGCAATCATGGGGT CTAGTCTCTGGAGACCTGTTGTCCTTGCTTCACCTGTCCCTAACACACGTGCAAGAAGGCAGAGTGACAG TGAGTCAGGAGTCTCAGCGGTCAAAAACCCTGGTTTCCTCCTGTTCTCGTGTGCTGAAGAAATTGAAGGC AAAGCTGCAGAGCCTCAAAACAGAATGTGAGGAGGCAAGGCACAGCAAGGAAATGGCCCTTAAAGGCAAG GCTGCGGCAGAGGCAGTACTGGAGGCTTTCCGTGCACATGCCAGCCAGCGCATCAGCCAACTGGAACAGG GTCTCACATCTATGCAGGAATTCAGAGGCCTTCTACAGGAGGCACAGACCCAACTGATAGGGCTTCACAC AGAACAAAAGGAGCTGGCTCAGCAGACAGTGAGTCTGAGTTCTGCCTTGCAGCAGGACTGGACATCAGTC CAATTGAATTATGGAATATGGGCAGCTTTGCTGAGTTGGTCTCGAGAACTCACCAAGAAACTCACAGCCA AGAGCCGGCAGGCCCTTCAGGAACGTGATGCTGCAATTGAAGAAAAGAAGCAGGTTGTGAAGGAAGTGGA ACAAGTCTCTGCCCATTTAGAGGACTGTAAAGGTCAAATCGAACAACTGAAGTTGGAAAATAGTCGCCTT ACTGCAGATCTCTCGGCTCAGCTGCAGATTCTGACTAGCACAGAGAGTCAGCTAAAGGAAGTACGGAGTC AGCATTCCCGCTGTGTCCAGGACCTGGCCGTGAAGGATGAATTGCTGTGTCAACTTACCCAGAGCAACAA GGAGCAGGCTACTCAATGGCAAAAGGAAGAAATGGAACTGAAACACATACAAGCAGAACTGCTACAGCAG CAGGCTGTGTTGGCTAAGGAGGTCCAGGACCTGAGGGAGACTGTGGAGTTTATAGATGAAGAAAGTCAAG TTGCTCACCGGGAGCTGGGCCAGATTGAGAGTCAGTTGAAAGTCACGCTAGAGTTACTGCGGGAGCGCAG CCTGCAGTGTGAGACCCTCAGGGACACTGTGGACAGCCTGAGGGCTGAGCTGGCCAGCACTGAAGCAAAG CATGAGAAACAGGCCCTAGAGAAGACACACCAGCATTCTCAAGAGCTGCGGCTACTGGCTGAGCAGCTGC AGAGTCTCACCCTCTTCTTACAGGCAAAACTCAAGGAGAACAAGGCTGAATCAGAGATCATTCTGCCCAG CACAGGCTCTGCTCCAGCCCAGGAACACCCTCTGTCCAATGACAGCAGCATCTCAGAACAGACCCCGACA GCAGCAGTAGATGAAGTGCCAGAACCAGCTCCTGTGCCATTGCTTGGAAGTGTTAAGAGTGCTTTCACCC GAGTAGCCTCAATGGCTTCCTTTCAGCCTACAGAGACCCCAGACTTGGAGAAGAGCCTGGCAGAAATGAG TACTGTGTTACAAGAGCTTAAGAGCCTGTGTTCCCTGCTGCAAGAGTCTAAAGAGGAGGCCACTGGGGTC CTGCAGAGGGAAATCTGTGAACTACACTCGAGACTACAGGCCCAAGAAGAAGAGCATCAGGAAGCCCTGA AGGCAAAGGAAGCAGACATGGAGAAGCTGAACCAGGCCTTGTGCTTGCTCCGCAAGAATGAGAAGGAGCT CCTGGAAGTGATACAGAAGCAGAACGAGAAGATCCTGGGGCAAATAGACAAGAGCGGCCAGCTCATAAAC CTCAGAGAGGAGGTGACCCAGCTCACACAGTCACTTCGGCGTGCAGAGACAGAGACTAAAGTGCTCCAGG AAGCCCTGGAAGGCCAGCTAGATCCCAGCTGCCAGCTGATGGCTACTAACTGGATCCAGGAAAAAGTGTT TCTCTCACAGGAGGTGAGCAAGCTGAGGGTTATGTTCCTGGAGATGAAAACTGAGAAGGAACAGCTGATG GACAAGTATCTGAGCCATAGGCACATCCTGGAGGAGAACCTTCGGCGCTCTGACACAGAGTTAAAGAAAC TCGATGACACAATTCAGCATGTCTATGAGACTCTGTTGTCTATCCCAGAGACTATGAAGAGTTGCAAGGA GTTACAAGGATTGCTAGAATTTCTGAGCTAAGAAACTGAAAATATTGGAACGCAGTGGTGTGCACCTGTA GTTCCAGCTGCTCCAGACGCCAAAGTTGGAGTGCTGCCAAATCCCAGGAATTTGGGAACCTAACACAGAC AACACAGCAAAAACCCTGAAAAATGAATTCCACTCCACTCCAGTTTCCTTCAGGGCTCTTTATCCCAACC CTTATCCCAATAATAAGACCAATTGGCATAGAGCCGACCATGTTTTATGTTATTTAAATAAAGTGTATTT AATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:30058496 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30058496 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004999 same experiment
	EMAGE:31030 same embryo
	EMAGE:30642 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source