EMAGE mouse gene expression spatial database

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EMAGE:30684

Meg3 maternally expressed 3 ( MGI:1202886)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003621_01	euxassay_003621_02	euxassay_003621_03	euxassay_003621_04	euxassay_003621_05

euxassay_003621_06	euxassay_003621_07	euxassay_003621_08	euxassay_003621_09	euxassay_003621_10

euxassay_003621_11	euxassay_003621_12	euxassay_003621_13	euxassay_003621_14	euxassay_003621_15

euxassay_003621_16	euxassay_003621_17	euxassay_003621_18	euxassay_003621_19	euxassay_003621_20

euxassay_003621_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9215
Entity Detected:	Meg3, maternally expressed 3 ( MGI:1202886)
Sequence:	sense strand is shown >T9215 AAACAAACAAACAAACAAACAAACAACATTCATTCAACAAACATTTTCTGAGACACTGACCATGTGCCCA GTGCACCAGGCTATGGCTGAGGTACAAAGACAATGAGAACAGTCTCTTTTTGGAGGCTAATTAGTACTCT CATTTCTACCAGCACTTTCTTTGGCCTTTTAAACCAACCATTTTTGTCCAGACAAGACCAAAAAAAAAAA AAAAAACAAAAACAAAAAAACAAAACAAAACAAAAAACCCAAAAAAAACAAAAAACAAAACAACTGGGTT GCTTTAGGGAAGCCCGGCTTGGAGTGGACAATGGTGTCCAGGCCCCTGAAAGGGGCTGATTGGATTATGA GGCAAATGGAGGCAGAAGGAGCACGCAGGAGACAAATGCAGTGGGCGGAGACCCGCTCTGAGTTAATCAG GCTGATTGAAGGGACCTTTTCTGTCTTGCCGAGTGGCCCGGGGGGGCTCTCACTAGTGCACTGCTGTGTG CACATGGAGACTGGAGCTACCCTGGTCCCTCTCTGGCAACTGTTCATTCATTTGATGCTCACAACTCCCT GTCCTGACCCAGGGCCGGCCTGCCATCACGCAGGGAAGCAGAGGTCGCCAAGCGGTTTCCGACGGGGCCC CCCCACATAACGTTAGCCTCGCGGTCTCTTCGGTTTTAGCAGCAGTGGACATGAACCTGTTTCCAGCCTG CATCCTCCAGTTCCTCTCGGGACTCCTGGCTCCACTGTGCTCACCTGGAGCCCCGCCAGCCCGCCTCCCC CTGTCCTCCTTCCTCGCCACTCCAGTGTCTGTCTTTCCTTCACAGCCCCGGCTTCTCGAGGCCTGTCTAC ACTCGCTGCTTTCCTTCCTCACCTCCAATTTCCCCTCCAACCCACTGCTTCCTGACTCGCTCTTCTCCAT CGAACGGCTCTCGCTCAGGCCTGTCGCGTCTTCCTGTGCCATTTGCTGTTGTGCTCAGGTTCCACGAGCT GCCCATCTCCACAGAAGAGCAGCTGGCATTGCCCACCGGCCATGCCGGCTGAAGAAAAGAAGACTGAGGA CCCCAGGATGCCCAGCGCGAGGACCCCAGGAAGCCCAGCGCGAGGACTCCACCCACGACGCCCAGCGCGA GGACTTCACGCACAACACGTTGCAACCCTCCTGGATTAGGCCAAAGCCATCATCTGGAATCCTGCGTGGG ACCCTGGACACACGGACACAGACACCTGCCCCCAGGACCCTCCAACTGTAAATCGATTGGAAGCGCCCTT GTGAGCTGGGGCCTTCAAAGGGCTGAGGAGAAAATGCAGGCCGAGAGACCAGCTTCTCAAATGCTTCAAG CCTCCATGACCTGAGCTCACCCCCTGGAAATGTCTGGAAAACATAATGGGCAGGTTTTCTGTCTTCAAAG TTTCCATCAAAACCTCTTCAAGTTCTTTATTGTTTAGGACTGAGACACTTGGGGACAGTAATGGGGACTT TCTTTTGTATCTGTCTTGAAAGGCCAAAATATTTTTATATTGCTGTAGACAAAGCCACCTATTTACAAAT GGACTCTTGTTCCGTCGTTTCCCACCAGGAAGACCGTACTATGTTTGTGTCTCTATGTATTCTGGGGTCT TGAAACAGGTTTCTCATGGGGATGGCCATTCACTAGAGCCCAGAGGGGCAGAAGGGGAAGTGTCTACCCT GCCAAGGGGGTCTGGGGAAGGAGGGGGGCTAGTCTCAGATTTGCTCCTTGTATGTCTAGGGTAAACCCTG GGAGAATCTGGTGGTAAAAGAACATATGCTGTGTTTGCCCCACATCTGCCATTTGTGTGTGTTTCTGGGC TACGGGTTTGCACACTTTGGTGGTTAACATGGTGGGAGGAAGTAAGAAGGGCCAAGAGGGCACGGGTGCT CTGGTTTTCCTGCTGGGAGGCTGTTTCACTGGTGTGTGTTACATCACATAATGCATGTTTAGCTGGGTGA GTGACAACAGTATGAAGGGACAGAGAAACAAATAGGTGTAGAAAGAGAGTCACAGAGCACACAGTCACCA TTCTGTGCCCAGGCGTTCATTAAATGTTCAATAGAATGCTGAGCTCTGATAGACAGAGCGGGCCATGGAC ACACTGGTATTGGGTCATCATGGACCTAAGGAGTTTTTTTAATTGAAGAAGGAATCTTACATAACAGAAG AAAGAATAAGAAGAGCTGTCAAAACGAGCTCTGAGGTTTTTGAAGCCCCTTAATATAAACAGACAGACAA TGGCGTGATCAGGAAAGGGAGAAAACAAACAAACAAACAAACAAGATGCTTACAGAAATACGAGAGGACG TGTGAGCATGCAAAGAGAACCCAGCTAGGTTAGTATCGGGGTGCTGGTGGATCGTTAATCCTGTCTGGGT ACCCCAGCTCCAAGGGGATGAGAGGCAGCAAGTGAGAGGACACATAGGAAACTGTAACAAGACAGAGGCG AGCAGATGGGGAGCTTCAGCGGAGTGTTAAGCTAGGCTTCGCAATCTGACCACTTAACTCTCCCAGCAAA GAAAAGAAAGGCTTAAATACTAAAAAGAAGGAAAGCTGGAAGAAAGGATGGTAGATGTGAAAGGCATAGA GGATAGAAAGCAATAGGGAGTGGAGTGCAGGTGCTGTGGAGAGACGCTTGCAGGTGACTCAATTCACCAG CCTGTGCAGCTGAGAAAGACAGCCAGTGACAAAGGAGAAGAAGAAAGGTAACAATACACTCACAAATAGG TAGATGAATGTAGCTGGAAGACGGGGCCTGGCTAGGGACGGGATGAGAGGCTGTGTCAGCAGACTGGTTA ATTCCCATCTGTGCTTCTGAGATGGACAATGACCCACCCGACCAAGAGACCTTTCAGGCATCAATATGTC AGGGAGGGCTTCAATGGGGCAATGGAGGCGAGGCCCATTAATAGCAGATTATGATGTATCCAATTAGGTG CACCTGCCCTCTGGAAGTGCAGAAACCAGTATTGAAATGGAAAGAAAGACTGAATGGAAGTCCGAGAGTA CAAATGAATGGATGGGAAAAAAGATTTGCATTCGCTCTTAGGGGGAGGGTGCTGCTGAGCCCCAGGGTCA TCTGATCTCCCGGAGAATAAAAGGGCGAGGGAGTGATAGAGAGTAACAATAAGATGGAGAAGAAATGAAA AGGAGTACAGAGAGAATAAGTGGGGACAATGAAGTCTAGGAGCGCGGCTTGTTTAACACAAGTCATCCAT ATTAATTCAAAATTGAGGCCCTGGGATGGGATGGGCTGTGTCCAAGGGATGAAGCTGTTGCCTAGCATGT GCCAAGTTCTGGGTTCCATCTCCGCCTGGCAAGACAAACACAGGAAACAAAATTATAGCCCAGAGGTTGT AAATGTTGTTTTTGATGGTTTTGGGACAAAGAGCTAGATCGGTTGGAAGATAGAGAAATAGGAAGAGGTG GGAAAGAGTTAGATGAGCACAAGAGGAAGCAGCAGTTCTTAAGATAAGTGTTTAAATATGGTGAATGCGT AGCTCTTGGGTGTGTCAAGATGAAAATAACGAAAAGAAACAAGTTTAGGGATGACGGCACAGGGATGAGC GGAAGCGGTGACTTTAGTTAATCACCGTGGCCAACTCAAGCCCTGTCAGGGATTTAAAAAAAGGTACAAA GGCGATAAAGGAAGACACATGCAATACGGAAGAGAGAGACAGGACAGACAGACACAGCGAAAAGGCTGAG GACATGCCTGAGAGGGTCGCGAAGGGATGAGAGAGAGAACAGCGAGAATTCTGCTTAATTCAAAACCGGG CTCCTGAGATAGCTAAATATCTCCTGAGCCCAAACACCAGCACAGGAGATTAAATAACAAAAGAGCTGCA GGCAGACCCAAGTGGAGAAGGCTTGGGACCCTCAGATCAGGCTTCAACTAAATCTGTGCACCTGAGGTCC ATAAATAAAAGAATAAATATTGAAATAAAGGAGTGACAGAAAGGATGAATGAGCACACATGACTGAATTA GAAATGGAAATGGCTAGAGCAGCCAGTAAGGAGTGGCCCATGGGACTTCTGCTCATCTTATTCTGGGCAC CTGAGGTCCCTTCGTGAGAAGATGCAGGGAGAAGAGGGGAAGGGAAAGGCATGAGAGCAAATGAAAGAGA GGCGAGGAGAGGAAATGAGAAAGTGGCAGAGAGGGGAAGCGATCGGTGGGTGCCTCGGAAGAGGAATGTC CACGAGGATTAATAACGAGGCTCTCATTAATCCTAAAGATCGATGAGGTAATCCAGACCCAGGTTCTTAA GGGGAAGGAGAATGGAGAGAATTAAAAAAAAAAGACGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831921 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831921 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003621 same experiment
	EMAGE:29868 same embryo
	EMAGE:30646 same embryo
	EMAGE:30682 same embryo
	EMAGE:30689 same embryo
	EMAGE:30654 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source