EMAGE mouse gene expression spatial database

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EMAGE:30897

Cilp ( MGI:2444507)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004028_01	euxassay_004028_02	euxassay_004028_03	euxassay_004028_04	euxassay_004028_05

euxassay_004028_06	euxassay_004028_07	euxassay_004028_08	euxassay_004028_09	euxassay_004028_10

euxassay_004028_11	euxassay_004028_12	euxassay_004028_13	euxassay_004028_14	euxassay_004028_15

euxassay_004028_16	euxassay_004028_17	euxassay_004028_18	euxassay_004028_19	euxassay_004028_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

tongue

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 10 11 12 16

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20

lower lip

moderate

regional

moderate expression: see section 08 weak expression: see section 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4446
Entity Detected:	Cilp, ( MGI:2444507)
Sequence:	sense strand is shown >T4446 CCCAGCTAAGAGTCTTGCTCAGGACACGAAAGAAGACATCTTCTTCCCTTTCCTCTGCACCCGACTTAGT GATTAAAGATCATTGGCTCTATGAAATATCTCTGGGGAACTACAGGTTCAACTTTGTGAAGCAGCGTCAC TTATCTGTCACACTTCTACAAGGGAGAGATGGCAGCAATCAAGACTTGGGTATTCTCTTTCCTGGTCCTT GAGGTCACCACCGTGCTGGGGAGACAGACGATGCTTGCCCAGTCAGTGAGAAGAGTCCAGCCTGTGAAGA GGACCCCCAAGACCCTTGCCAAGCCTGCCGACTCCCAGGAGAGTCCCGGAGAGTGGACAACATGGTTTAA CATCGACCACCCAGGTGGGCAGGGTGACTATGAGCGGCTGGATGCCATTCGCTTCTACTATGGGGAGCGT GTGTGTGCCCGTCCCCTGCGGCTAGAGGCCCGGACCACTGACTGGATGCCTGCGGGCAGCACTGGCCAGG TGGTCCACGGCAGTCCCCGTGAGGGCTTCTGGTGCCTCAACAGGGAGCAGAGACCAGGCCAGAACTGTTC CAATTATACCGTGCGCTTCCTCTGCCCACCAGGGTCCTTGCGAGGAGATGCAGAGCACATCTGGAGTTCC TGGTCTCCCTGGAGCAAGTGCTCAGCTGCCTGCGGTCACACTGGGGTGCAGACCCGTACCCGCACCTGCT TGGCACAGACAGTGTCACTGTGCAGTGAGGCGACCGAGGAGGGCCAGCTCTGCATGAGCCAGGCCTGCAC AGCTTGTGACCTGACCTGCCCCATGGGCCAGGTAAATGCTGACTGTGATGCCTGCATGTGCCAGGACTTC ATGCTTCACGGGGCCATCTCCCTCCCTGGAGGTGGCCCAGCTCCAGGAGCCGCTGTCTACCTGCTGGCCA AGGCACCAAAGATGCTGACCCGAACAGACAGCAGCGGGAGGTTCCGAGTTCCTGGCTTGTGTCCTGATGG CAAAACCATCCTGAAAATCACCAAGACCAAGTTTGCCCCAATTATGATCACGATGCCCAAGACTAGCCTG AAGTCAGCTACCATCAATGCGGAGTTTGTGAGGGCAGAGACCCCATACATTGTAATGAACCCTGAGATGA AAGCACGTCGGGCTGGGCAGAGTGTGTCTCTGTGCTGCAAAGCCACGGGGAAACCCAGTCCGGACAAGTA CTTCTGGTACCACAACAACACACTGCTGGATCCCTCCCTCTATAAGCACGAGAGCAAGCTGGTGCTGAGG AACCTGCAGCAGGACCAAGCAGGGGAGTACTTCTGTAAGGCTCAGAGTGATGCTGGGGCTGTGAAGTCCA AGGTCACCCAGCTCACTGTCATTGCACATGATGAGACTCCTTGCAACCCAACCCCAGAGAGCTACCTTAT CCGGCTGCCCCATGACTGCTTTCAGAACGCCAGCAATTCCTTCTACTACGACGTAGGCCGCTGCCCGATC AAGACCTGTGCAGGGCAGCAGGACAATGGGATCCGGTGCCGAGATGCTGTGGAGAACTGCTGCGGCATTT CCAGAACAGAAGAGAGGGAGATCCAGTGCAGTGGGTACACACTGCCCACCAAGGTGGCTGTGGAGTGCAG CTGCCAACGATGCGCAGAGACACGGAGCATCGTCCGGGGCCGAGTCACTGCTACTGACAACGGGGAGCCC ATGCGCTTTGGCCATGTGTACATGGGGAATAACCGCGTGAGCATGACTGGCTACAAAGGCACCTTCACAC TCCACATCCCCCAGGACACGGAGAGGCTGGTGCTCACATTTGTGGACAGGCTGCAGAAGTTTGTCAACAC AACCAAAGTGCTGCCCTTCAACAAGAAGGGAAGCGCAGTGTTCCACGAGATCAAGATGCTTCGGCAGAAA GAGCCCATCACGTTGGAAGCCATGGAGACCAACATTATCCCCCTGGGAGAGGTGATTGGTGAAGACCCTG TGGCTGAGCTGGAGATTCCATCCAAAAGTTTCTATAGGCAGAACGGGGAGCCCTTCACAGGAAAGGTAAA GGCCAGCGTGACCTTCCTGGATCCTCGGAACATCTCGACGGCCACAGCTGCCCAAAGTGACCTCAACTTC ATCAATGATGAAGGAGACACTTTTCCCCTTAGAACATATGGTATGTTTTCAGTGGACTTTAGAGATGAGG CCACTTCCGAGTCACTTAATGCTGGCAAGGTGAAGGTCCACCTTGACTCGACCCAGGTCAAAATGCCAGA GCATGTGCCAGCCATGAAACTCTGGTCGCTCAACCCAGACACAGGACTGTGGGAAGAGGAAGGGGATTTC AAGTTTGAAAGCCAAAGGAGGAACAAGAGGGAAGAGAGAACCTTCCTAGTGGGCAATATGGAGATCCGGG AGAGAAGGCTCTTTAACCTGGACGTCCCTGAAAGCCGAAGGTGCTTCATTAAGGTGAGGACTTACCGGAG TGAGAGGTTCCTGCCCAGTGAGCAAATTCAGGGAGTTGTGGTCTCAGTGATCAATCTGGAGCCCAGAACT GGCTTCTCATCTAACCCCAGGGCCTGGGGCCGATTTGACAGTGTCATCACAGGGCCCAATGGGGCCTGCC TGCCTGCCTTCTGTGATGATCAGTCCCCCGATGCTTACTCTGTCTATGTCTTGGCAAGCCTTTCTGGAGA AGAACTAGAGGCAGTTGAGTCTTCTCCTAAATTCAACCCAAATGCAATCGGTGTCCCTCAGCCTTACCTC AACAAACTTAAATACCGTCGGACAGACCATGAGGATCCACGGGTTAAAAAGACGGCTTTCCAAATCAGTA TGGCCAAACCAAGGCCCAACTCAGCTGAGGAGAGCAATGGGCCTATCTATGCCTTTGAGAACCTTCGGGC GTGTGAGGAGGCACCCCCCAGTGCAGCTCACTTCCGGTTCTATCAGATCGAGGGGGATCGCTATGACTAT AACACAGTTCCTTTCAATGAAGATGACCCTATGAGCTGGACTGAAGACTACCTGGCATGGTGGCCCAAGC CAATGGAGTTCAGGGCCTGCTATATCAAGGTTAAGATTGTGGGGCCACTGGAGGTGAATGTACGATCCCG TAACATGGGAGGCACCCACCGGCAGACTGTGGGGAAGCTCTATGGGATCCGGGATGTGAAGAGTACTCGG GACAGAGACCAACCTAATGTCTCATCTGCCTGCCTGGAGTTCAAGTGCAGTGGAATGCTGTATGACCAAG ACCGTGTAGACCGCACATTAGTAAAGGTTATCCCCCAGGGCAGCTGCCATCGAGCTAGTGTTAACTCTAT GTTGCACGAGTACCTGGTCAACCACCTTCCCCTGGCAGTCAACAATGACACCAGCGAGTATACTATGCTG GCACCCCTGGACCCTCTGGGCCACAATTACGGTATCTATACTGTTACTGATCAGGACCCTCGTACAGCTA AGGAGATTGCGCTCGGCCGGTGCTTTGATGGCACTTCCGATGGCTCCTCCAGAATCATGAAGAGCAATGT GGGAGTTGCCCTGACCTTTAACTGTGCAGAAAGGCAGGTAGGCCGCCAGAGTGCCTTCCAGTACCTCCAA AGCACACCGGCCAGGTCCCCAGCTACAGGCACTGTCCAAGGAAGAGTACCAGCCATGAGGCAACAACGGG CAAGCAGGGGTGGCCTACGCCGGCGTGGAAGCATGGCCCCTCTGAGATTTTCTGGTGTTGCTCAACAACC TCTGAGCAACTAAGTCTTGTGGTTCTTAGTCCTTTCTGTCCATCTCATGTGACAGCTGTCGTGAGACTGA TGCACAGACTGTCACTTGTTAATTTAAACATATCTGGTTTGGTGCAGTTTGTTTCTTTGTGCCTTTACTT ACTGTCTCTGCCTCATGATGCTGATTGCCACAGGGCCCCCACGATGACAAAACAAAGATTCTCCTCTTAA AAAGAAACAAAAGGAATTGGTAAACGTCTGCAGCTTTGGCTGAAAACACACATCTTATTGTATGGTTTTG CCAGCCTTTACACTAATGATAATCTAATACTGAGGCTCAAGTGAACAACAATAAAACATATTTCTTGGCC ATGTTCCACAAGATGTAAGCAAAGTCTCTGTCACAGTCCATATATATATAAATGGTGGTGAATAAGGAAA TAAACAATGTTTTTATGTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4934956 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4934956 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004028 same experiment
	EMAGE:30943 same embryo
	EMAGE:30031 same embryo
	EMAGE:30947 same embryo
	EMAGE:30334 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source