EMAGE mouse gene expression spatial database

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EMAGE:30947

Nicn1 ( MGI:1913507)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004034_01	euxassay_004034_02	euxassay_004034_03	euxassay_004034_04	euxassay_004034_05

euxassay_004034_06	euxassay_004034_07	euxassay_004034_08	euxassay_004034_09	euxassay_004034_10

euxassay_004034_11	euxassay_004034_12	euxassay_004034_13	euxassay_004034_14	euxassay_004034_15

euxassay_004034_16	euxassay_004034_17	euxassay_004034_18	euxassay_004034_19	euxassay_004034_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19

cervical ganglion

moderate

regional

moderate expression: see section 07 08 14

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 15

thoracic ganglion

moderate

regional

moderate expression: see section 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4951
Entity Detected:	Nicn1, ( MGI:1913507)
Sequence:	sense strand is shown >T4951 GGGACGCCAATAAGAGCTAGTGGTTGGATGGAGACTGTGAGCGGTGATTTTGCTTGGTTGTAATCTCCCA AATCATGTCCCGTGTGTTGGTGCCCTGTCATGTGAAAAGCACTGTAGCCCTGCAAGTGGGCGACATGAGG ACCTCTCAAGGTCGGCCTGGCGTGCTGGTCGTCGATGTCACTTTTCCCAACATCGCGCCTTTCGAGTTGC AGGAGATCATGTTTAAGAATTACTACACAGCTTTCTTGAGCATCCGTGTTCGTCAACAAAGCTCCATGCA CACAGCAGCCAAGTGGGTAACTTGTCTTCGGGATTACTGTTTGATGCCTGACCCACACAGTGAAGAGGGA GCCCAGGACTATGTATCACTGTTCAAGCACCAGATGCTGTGTGACATGAACAGAGTGCTGGAGCTGCGTC TGATTCTGCGGCAGCCATCACCACTGTGGCTGTCTTTCACAGTGGAGGAACTGCAGATCTACCAACAGGG ACCAAAGAGCCCCTCCTTGGCGTTCCCCAAGTGGCTTTCCCACCCAGTGTCCAATGAGCAGCCTGCTCCC CGCCTTGAGGGTCTCCCAGATCCCAGCAGAGTGTCCTCTGAGGTGCAGCAGATGTGGGCACTGACAGAGA TGATTCGGGCTAGTCACACTTCCACGAGGATCGGCCGCTTCGACGTGGATGGCTGTTATGATCTGAATTT ACTCTCCTACACATGAACAGCAGCTTCTACGTGAGATGTTGGCTTTCTGTGCCCATCTAGATGGGGTTTG GCCACCAGAGGCCGTTCCCTGTGCATCCAAGTTCTATCTGGGCAAAAATCTGCAGATTGGGAATTCTTGA TACCCATCCAGTTGTTTTAGCATGAATTCCTAGTGGGGGCACTTCTGTCTCTGACTGTCTGACATGTAAA TTCAGTCTCTTCCTCTCAGAATTCACCGGTTCTAATTAGAATCCTTGAGCTCCTCTTGCTTGTCCTGCCA ACACGAACACCCCAGTCTGCTACCATTTCCCCCCATTGCTGAGATAAACCTGTCACTTGTAGCAAGGAAG CCACTGTTGTTTTGACACTCATGACCTGGGAAGCTTGAGGAAGACATACCCTCTATTCATTCCTCTGCTT CTGCTAGTAGCCCTTGTCCTTTTGTATTGTATCCATGAAAAGAAAATGTCCTAGAGCCAGGCATGGCACA CACCTTTCATCTTAGCATTCTGTGAGTTCAAGATCAGCCAGGTCTACACACTGAATCCCAAGGCCAATCA GGGCTACATAGTGAAACTCCATACTAAGAAAGGAAGGAAGAAAGAAAAGAAAGAAAAAAGGAAGAAAGAG GAGGAAGGAAGGAGAGAAAGAAAATAGCTCTCTTTGAAATTGTCTGCTTCCAGCCTGGGAGGCGAAGATG AGACTTCTGGGAGCTTGAGTGCTTAAGTCCCTACAATTGCTCCTGAAAAGTGTTCTGTGTCAGTTCTGGG TAGGTGCTGTTGGCACAGCTGGGGCCAGAGCACGAGTCCTCCCTTCGGACTATTTGTCATCAGATTCAGC ATGAAGTACATCCAACGATCCCTGTCCCTTCTCTCAGTTGCTTCTGCCCGTCATGCTCAGGGTGCTGAGA GAGCCCTGGGGGAGTGCGTCCAAGCCAACGTCTCACTGACACTCATTGAGGCTGAGGCCCTCCATTACCC CAGAGCAACCACTAAATGAGGAAGACTACTGCTCTTTCCTGTGATCAGATTGTTTCCCTCTAGTCCTTCC TGGCTTCGTCCCCGGGCAGTATTATTAAGCCGTGCTGCAAGGTGTCTCAGATCTGGAAAATTGGACACGA GTATATGCAACAGTTTTCTGGGAAAGTCTGGCCACCTTTGGTAGCTACCTTGCATCTCTGCAGTCAGCCC TCTTGAAGGTCCTGTCTTCACCTACAGGATCATAGTTAAGCCCCACTGACAGGGTAATTACCATAGTCCA GTGTTCAGGGAACAATGGTCCTCAGTACACTTGGGCAAAGAGGATCCTGAAGTGGCAGGGGCTATGCATA ATTCACAGCTGTGTTCCTAAGTTCCCAGAGCTGGAGCTAGTGTACGGCAAGATCAATAAATACTTGTGGA ATAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5133763 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5133763 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004034 same experiment
	EMAGE:30943 same embryo
	EMAGE:30031 same embryo
	EMAGE:30897 same embryo
	EMAGE:30334 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source