EMAGE mouse gene expression spatial database

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EMAGE:30909

Ovol1 OVO homolog-like 1 (Drosophila) ( MGI:1330290)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002082_01	euxassay_002082_02	euxassay_002082_03	euxassay_002082_04	euxassay_002082_05

euxassay_002082_06	euxassay_002082_07	euxassay_002082_08	euxassay_002082_09	euxassay_002082_10

euxassay_002082_11	euxassay_002082_12	euxassay_002082_13	euxassay_002082_14	euxassay_002082_15

euxassay_002082_16	euxassay_002082_17	euxassay_002082_18	euxassay_002082_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14

vibrissa

strong

regional

strong expression: see section 03 04 13 14 moderate expression: see section 02 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 05 06 08 09 10 11 12

hindgut

moderate

regional

moderate expression: see section 07 08 09 10

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 07 08 09 10 11 12 13 14 15 16 17 18 19

naris

strong

regional

strong expression: see section 06 08 09 weak expression: see section 07

foregut-midgut junction

moderate

regional

moderate expression: see section 06 07 08 09

stomach

moderate

regional

moderate expression: see section 05 06

kidney calyx

moderate

regional

moderate expression: see section 05 weak expression: see section 09 10 11 12 13

urethra of male

moderate

regional

moderate expression: see section 07

midgut

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

bladder

moderate

regional

moderate expression: see section 07

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4633
Entity Detected:	Ovol1, OVO homolog-like 1 (Drosophila) ( MGI:1330290)
Sequence:	sense strand is shown >T4633 CCACGCGTCCGAGCCAATAAAGCGTGAACCCGTCCGTCCAGCTCGCACTTGAAGACTTCGCGAGCAGCCG CGGGGACGCCAGTCGAGCTAAAGGACGCTGGCTTAGCGCTTCTGCGATCCCGGTGCACCTGGCTGCACCC TCTCTGTGGACACCGGCGACCTTCGGCGTAGTTGGAAACGCCACGCGTGGTCCCTGGTACACTCTGTCCT AAGAACGTGGACACGCCTCGGCGACTCTTTCCTTGAACTGTCCCTATTCCCGCGCCTCAACCCCGATCCG GTGGAAGAAAAGGTGGGACGACCACACGAACTCGACTTACCAGAGCGAACCGCGCCGGGCCAGGAGGAAC TGAAGATGCCTCGCGCGTTCCTGGTGAAGAAGCCATGCGTCTCCACGTGCAAGAGGAACTGGAGCGAGCT CCCGGACGAAGAGCGCGGCGAGATCTACGTGCCAGTCAGCCTCGGCTTCTGCCCACCACAGCCCTACCGG GAACCAGAGGCATCGGTGGCCGAGCCTCCTTCCTGCCCCCTGGCTTTGGACATGAGCCTTCGAGACTCCA GCTATAGCGTGGCTCCAGGGCCCTGCGTGGTGGCCCAGCTACCCTCTGAAGATGTGAGCCATCTGACAGA TCCCCAGAGCAGAGACCAGGGCTTTCTACGAACCAAGATGAAGGTGACCTTGGGGGACAGTCCAAATGGA GACCTTTTTACCTGCCACATCTGCCAGAAGTCCTTCACCCACCAGCGCATGCTGAATCGACACATGAAGT GTCACAACGACGTCAAGAGGCACCTCTGCACTTACTGTGGGAAGGGCTTCAATGACACCTTTGACCTCAA GAGACATGTCCGAACCCACACTGGTGTGCGGCCCTACAAGTGCAGTCTGTGTGATAAGGCCTTCACTCAG CGCTGCTCTCTGGAGTCCCACCTCAAGAAGATCCACGGAGTGCAGCAGAAGTATGCATACAAGGAGCGCC GGGCCAAGCTCTACGTGTGTGAGGAGTGCGGTTGCACATCCGAGAGTCAAGAGGGTCATGTCCTGCACCT CAAGGAACGCCACCCCGACAGCCCACTGCTGCGCAAGACCTCCAAGAAAGTGGCTGTGGCTCTGCAGAAC ACTGTCACGTCCCTGCTGCAGGGCAGCCCCCATCTCTGAGCAGCACAGGCCCTGGGAAGGACCAGGTTCA CTTCCCTGCTGCCCAGCCATTCACCTTCCTGCAACCTCTTGCTAGAACACCAGTGACTGGGACCAGCGCT CTAACACCTCACTCCAGGCACACTTGCACAATTGGGCACTGACTTCTGCTCTGTTTGGGGAGTCTGTCTT ACAGGCAGAGCACACCCTGAGCTTGGGACCATAGGTGTTTGTGTCCCAGCAGGAGTGAGGACAAATGAGC AAGAAGAACCACTTACAGAGACAGGCCCTGTTTCCTTGCAGCAAAACTTTGCAGATGTGCTAAGAGGGGG TCCTGAGTACAAGATGGGCAATGGATTCCCCTGGAGAGCAGGATGGAGACACCAGCAAAGAGCATCCCGG CAAACAACCGCAGTGGTGCTGGCAGCGGCCCTCGTGCATGCAGGGCTAACTCTCAGGGTTCCAAGGGCTC TCTGCCCTTTCATCAGAGACCCACATGTGAACAGCCGCGTGTATGTCACACTGCTCTGTGTGTGATTTCT TCACGTGTGCATGTGCGCTCTTGGTCTTTCCACTTATTGCCTCGTTCGTAAGAAACCAACCATAAGGTGC CAAGGAGGTTTTATTCCTTTTTTTTTTAAAGATGACAAATGTACAGATGTTAGTACAGATGTTAATGTAC AGATGTTAATATATTTTTGGTGCCCACAGTAAGACTCAGGGGGGGAGCTGGCTTTTCTCCACCCCCACTG GTTCTATTTATCCTGGACATTTCAAACCTCCTCTGTGCCTTGGTCCTGGGGTGGTTTTGCAGACCCACCC TGTTCTCTGAGATGTTTCATTAAAGACCTTCCTCCTGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5006712 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5006712 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002082 same experiment
	EMAGE:30933 same embryo
	EMAGE:29483 same embryo
	EMAGE:29497 same embryo
	EMAGE:29257 same embryo
	EMAGE:31325 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source