EMAGE mouse gene expression spatial database

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EMAGE:31325

2810417H13Rik RIKEN cDNA 2810417H13 gene ( MGI:1915276)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002064_01	euxassay_002064_02	euxassay_002064_03	euxassay_002064_04	euxassay_002064_05

euxassay_002064_06	euxassay_002064_07	euxassay_002064_08	euxassay_002064_09	euxassay_002064_10

euxassay_002064_11	euxassay_002064_12	euxassay_002064_13	euxassay_002064_14	euxassay_002064_15

euxassay_002064_16	euxassay_002064_17	euxassay_002064_18	euxassay_002064_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 08 09 moderate expression: see section 07

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4632
Entity Detected:	2810417H13Rik, RIKEN cDNA 2810417H13 gene ( MGI:1915276)
Sequence:	sense strand is shown >T4632 CCACGCGTCCGCAGTTGGGGTGACGGTGAAAGTTGTCTCTTCTTGTGTGAACATGGTGCGGACCAAAGCA AACTACGTTCCAGGAGCCTACAGAAAAGCGGTGGCTTCTCAAGCCCCTAGGAAGGTGCTTGGCTCCTCCA CCTTTGTCACCAATTCTTCAAGTTCGTCGAGAAAAGCTGAAAATAAGTATGCAGGAGGGAACCCAGTCTG TGTGCGCCCAACTCCCAAGTGGCAAAAAGGCATCGGGGAATTCTTCAGGCTGTCCCCTAAAGAGTCTAAA AAGGAAAACCAGGCTCCTGAAGAAGCAGGAACCAGTGGCTTAGGAAAAGCAAAGAGAAAAGCTTGTCCTT TGCAACCTGATCACAGAGATGATGAAAATGAATAGAATTTACTCATCTGAATAATGTCTCCGGTTTTTCT ACATTACTTACATGTAAATTTGGGTAAATGAGTTTGTCCAATTGGCTTGTTGAGGGGAAAAAAATTAGGC TAAAATTTAGCCATTCAAAAGAAACCAGATGTAAGAATGGAGAATTTTTATAATTTGTAAATTTGAATTG CATGCTTACACTTATGCCCTATGCTCCTCCTTTCTTACCTGTTAAAGCTTCTTCTTAAAGCCAACGTTCC CTGGCACTCTACTTGCTACTCTACGGTCTTTGCTTAGATTTTTGTACTGATGCCATTTTATGGGCAGTGA TTATGGAAATGACGCCATATTGTTATGGCATGTTTGCTTTAAAAATTTGACTTAGGCTGGGTGTGGTGGC GCACGCCTTTAATCTTTAAGAGTGAGTTCCAGGTCAGCCAGGGCTATACAGAGAAACCCTGTCTCGAAAA ACCAAAAAAAAAAAAAAAATTTGACTTAGTCTTTCTTACATTAAAATTTTCAATATTACCTAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5006272 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5006272 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002064 same experiment
	EMAGE:30933 same embryo
	EMAGE:29483 same embryo
	EMAGE:29497 same embryo
	EMAGE:30909 same embryo
	EMAGE:29257 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source