EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30946

Mageh1 ( MGI:1922875)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005123_01	euxassay_005123_02	euxassay_005123_03	euxassay_005123_04	euxassay_005123_05

euxassay_005123_06	euxassay_005123_07	euxassay_005123_08	euxassay_005123_09	euxassay_005123_10

euxassay_005123_11	euxassay_005123_12	euxassay_005123_13	euxassay_005123_14	euxassay_005123_15

euxassay_005123_16	euxassay_005123_17	euxassay_005123_18	euxassay_005123_19	euxassay_005123_20

euxassay_005123_21	euxassay_005123_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mandible

moderate

regional

moderate expression: see section 07 08 09 10 11 15 16 17 18 19 20 21

sternum

moderate

regional

moderate expression: see section 09 10 11 12 13

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 13

spinal cord

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 09 10

retina

moderate

regional

moderate expression: see section 01 02 22

adenohypophysis

strong

regional

strong expression: see section 08 09 10 11 12 13 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 15 16 17 18 19 20

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

maxilla

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20 21

tail

moderate

regional

moderate expression: see section 09 10 11 12 13 14

cervical ganglion

moderate

regional

moderate expression: see section 06 07 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8576
Entity Detected:	Mageh1, ( MGI:1922875)
Sequence:	sense strand is shown >T8576 GCTTTGCAGGAGCCATGCCTCGGGGACGGAAGAGTCGGCGCCGCCGGAACGCAAAGGCAGCTGAAGAGAA TCGCAACAATCGCAAGAGCCAGGCCTCAGAGGCTTCAGAGACCCCGATGGCGGCTTCTGTAGCCCCGAGC ACACCCGAAGAATACCTGAGCGGCCCGGAGGAAGACACAAGCACCCTGGAGAAGGCCTCCAGTACCCCTT CAGAAGCTTCGAGCACTGCCCTAGTGCAAAAGCCGGTTACCCGGAGCAATTTTCAGGGCACCAAGAAAAG TCTCCTAATGTCCATATTAGCCCTCATCTTCATCATGGGCAACAGCGCAAAGGAGGCCCTGGTGTGGAAA GTGCTGGGGAAGTTAGGGATGCAGCCTGGGAGGCAGCACAGCATCTTTGGAGATCCGAAGAAGGTCGTTA CAGAAGAGTTTGTGCGCAGAGGGTATCTGATTTATAAGCCAGTGCCCCGCAGCAGTCCAGTGGAGTATGA GTTCTTCTGGGGCCCTCGAGCACACGTGGAATCTAGCAAGCTGAAAGTCATGCATTTTGTGGCAAGAGTT CGGAACCGATGCTCCAAAGACTGGCCATGTAACTATGACTGGGATTCAGATGATGATGCAGAGGTTGAGG CTATTCTCAATTCGGGTGCTAGGGGTTATTCTGCTCCATAGAGAGATCCTAGGCAAACCCTTAGGAGTGG AAAAGAGAATCCAAAGTATCTGCTCCATGGAGTAGATAGGCAGGGTCCTGAATTCAAAAGGTTGTGGAGG GTGGGGAGGGCACTGTATTTGGTATTTGTGACCAGTGCTAATTGTTAAACTGCAAAGTAGTGTTTGCTAT AGATATTGTTATCTTGTATTCATTTTATAACACTGTTGACTAAGAATCACAATATGTTTAAATATATACT TGAGGAAAAGTTTATGTTATTTTTTTCTGTCGTTGGCATTTGATATTAACAGCTGTGCTAACCTTATTAA ATGAAGTCTTTGTGTCATGTTGTGTGATGGATAGAAAGTACCACTGAGAGGCTGTTCTTTGAAAGTTTGG AATAACCAGTAAAGTGTGAGTGGAAGAAGATTACAGATTTGTTCTGTCTTTCCCTTGTGTCTGATGTCTT GTAATGGAACACATTTGTAAATATGCTCACTGTGCAAATAGTATAAATAAAAGCATAGTAAACAGAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5714800 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5714800 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005123 same experiment
	EMAGE:30857 same embryo
	EMAGE:30866 same embryo
	EMAGE:30893 same embryo
	EMAGE:29224 same embryo
	EMAGE:31337 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source