Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30968

Mif macrophage migration inhibitory factor ( MGI:96982)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968
euxassay_004929_01 euxassay_004929_02 euxassay_004929_03 euxassay_004929_04 euxassay_004929_05
EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968
euxassay_004929_06 euxassay_004929_07 euxassay_004929_08 euxassay_004929_09 euxassay_004929_10
EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968
euxassay_004929_11 euxassay_004929_12 euxassay_004929_13 euxassay_004929_14 euxassay_004929_15
EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968
euxassay_004929_16 euxassay_004929_17 euxassay_004929_18 euxassay_004929_19 euxassay_004929_20
EMAGE:30968 EMAGE:30968 EMAGE:30968 EMAGE:30968
euxassay_004929_21 euxassay_004929_22 euxassay_004929_23 euxassay_004929_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6905
Entity Detected:Mif, macrophage migration inhibitory factor ( MGI:96982)
Sequence:sense strand is shown

>T6905
GGCTTGGGTCACACCGCGCTTTGTACCGTCCTCCGGTCCACGCTCGCAGTCTCTCCGCCACCATGCCTAT
GTTCATCGTGAACACCAATGTTCCCCGCGCCTCCGTGCCAGAGGGGTTTCTGTCGGAGCTCACCCAGCAG
CTGGCGCAGGCCACCGGCAAGCCCGCACAGTACATCGCAGTGCACGTGGTCCCGGACCAGCTCATGACTT
TTAGCGGCACGAACGATCCCTGCGCCCTCTGCAGCCTGCACAGCATCGGCAAGATCGGTGGTGCCCAGAA
CCGCAACTACAGTAAGCTGCTGTGTGGCCTGCTGTCCGATCGCCTGCACATCAGCCCGGACCGGGTCTAC
ATCAACTATTACGACATGAACGCTGCCAACGTGGGCTGGAACGGTTCCACCTTCGCTTGAGTCCTGGCCC
CACTTACCTGCACCGCTGTTCTTTGAGCCTCGCTCCACGTAGTGTTCTGTGTTTATCCACCGGTAGCGAT
GCCCACCTTCCAGCCGGGAGAAATAAATGGTTTATAAGAGAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3978685 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3978685 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004929 same experiment
 EMAGE:30206 same embryo
 EMAGE:30130 same embryo
 EMAGE:30062 same embryo
 EMAGE:31018 same embryo
 EMAGE:31016 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS