EMAGE mouse gene expression spatial database

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EMAGE:31016

Ncf2 ( MGI:97284)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006090_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

single cell

moderate expression: see section 09 10 weak expression: see section 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6555
Entity Detected:	Ncf2, ( MGI:97284)
Sequence:	sense strand is shown >T6555 GGCTGGGACCTTGAAGCCTGGAGCGCCTACCCGGCAGCTCTGAGAGAGACACCTTGAACTACCATCCTGC CTTCTGGTAAGCACCTAACCATGTCCCTGGCTGAGGCCATCAGACTCTGGAATGAAGGGGTGCTCGCAGC CGACAAGAAGGACTGGAAGGGGGCCCTGGAGGCCTTCAGCGAGGTGCAGGACCCCCACTCGAGGATTTGC TTCAACATAGGCTGCGTGAACACCATCCTGGAAAACTTGCAGGCAGCCGAGCAGGCCTTCACCAAAAGCA TCAACAGAGACAAGCACTCTGCAGTGGCCTACTTCCAGAGAGGAATGCTCTACTACAGAATGGAGAAGTA CGACCTTGCTATCAAAGACCTTAAAGAGGCCTTGACGCAGCTTCGTGGGAACCAGCTGATAGACTACAAG ATCCTGGGGCTGCAGTTCAAGCTGTTTGCCTGTGAGGTATTGTACAATATTGCTCTCATGCATGCCAAGA AAGAGGAATGGAAGAAAGCAGAAGAGCAGTTGGCATTGGCAACCAACATGAAGTCCGAGCCCAGGCATTC CAAGATCGACAAGGCCATGGAGAGCATCTGGAAGCAGAAGCTGTTCGAGCCCGTGGTGATCCCTGTGGGT CGGCTGTTCCGTCCAAATGAGAGGCAGGTGGCTCAGCTGGCCAAAAAGGACTATCTGGGCAAGGCTACGG TTGTAGCATCTGTGGTTCACCAAGACAACTTTTCTGGCTTCGCCCCTCTGCAGCCGCAGTCAGCAGAGCC TCCTCCCAGACCCAAAACCCCAGAAATCTTCAGGGCTCTGGAAGGTGAGGCACACCGCGTATTGTTTGGC TTTGTGCCGGAGACGCCAGAAGAGCTACAGGTCATGCCTGGGAACATCGTCTTTGTCTTGAAGAAGGGCA GTGATAACTGGGCCACAGTCATGTTCAATGGACAGAAGGGGCTTGTCCCCTGCAACTACCTGGAGCCAGT TGAGCTTCGGATTCACCCTCAGTCGCAGCCCCAGGAAGATACCTCTCCAGAATCTGATATTCCACCACCT CCTAATTCTAGTCCCCCAGGAAGACTCCAGTTGTCACCAGGTCACAAGCAAAAAGAGCCCAAGGAACTGA AGCTCAGCGTGCCTATGCCTTACATGCTCAAGGTGCATTACAAATACACAGTGGTCATGGAGACGCGGCT TGGCCTCCCCTACAGCCAGCTTCGGAACATGGTGTCTAAGAAGCTGGCGCTCTCGCCAGAACACACTAAA CTGAGCTACCGGCGTCGGGACAGCCACGAGCTTCTGCTCCTGTCCGAAGAAAGCATGAAGGATGCCTGGG GCCAAGTGAAAAACTACTGCCTGACTCTGTGGTGTGAGCATACGGTGGGTGACCAAGGTCTTATTGATGA ACCCATACAAAGGGAAAACTCAGACGCCAGTAAGCAGACTACGGAGCCTCAGCCTAAGGAGGGGACCCAG GTGGTAGCAATCTTCAGTTATGAGGCTGCCCAGCCAGAAGACCTGGAATTTGTGGAAGGAGATGTAATCC TGGTACTGTCACATGTGAATGAAGAATGGCTGGAAGGGGAGTGTAAAGGGAAAGTTGGCATTTTCCCGAA GGCTTTTGTTGAAGGATGTGCAGCCAAGAATTTGGAAGGCATTCCCAGAGAAGTCTAGGATCTACAAAGC TGAAGCAAAGGGTTGTTTTTCCCCTTGCAAGACTTACCCATCTCTGCTGTGCGCTATACAGACACACCAG TTTGGAAATGTGGCCTAACTTACCATTTCCCTATTAAAATTGAACCTGTCACACAGCAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3592460 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3592460 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006090 same experiment
	EMAGE:30968 same embryo
	EMAGE:30206 same embryo
	EMAGE:30130 same embryo
	EMAGE:30062 same embryo
	EMAGE:31018 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source