EMAGE mouse gene expression spatial database

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EMAGE:30972

Mrpl38 ( MGI:1926269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_001892_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 07 08 10 11

vibrissa

moderate

regional

moderate expression: see section 07 08 18 19

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 14 15 16 17 18 moderate expression: see section 12 13

midbrain ventricular layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T656
Entity Detected:	Mrpl38, ( MGI:1926269)
Sequence:	sense strand is shown >T656 TCCTCGAGNCTGTTGGCCTACTGGCTCCGGCCACCTCTGTCTGGGTCCTACGGGCGTCTTTGGCGACCTG GGGGAAAGATGGCGGCGCCCTGGTGGCGTGCGGCGTTTTTCGGAATTGGGAGATGCCGGGGCTTCAGCAC CTCGGCCTTCCTCAGCCGCCGGACACCCCCGCTCGGGCCCATGCCCAACGAGGACATCGACGTGAGCAAC CTGGAGCGGCTGGAGAAGTACCGGAGCTTCGAGCGGTACCGCCGCCGGGCGGAGCAGGAGGCGCGCGCCC CGCACTGGTGGCGGACCTACCGGGAGCATTTCGTGAAGGAGACAGATCCCAAAGACAAAATTGACATTGG GCTACCCCCACCCAGAGTCAGTCGGACCCAAAAGCTACTGGAGCGGAAGCACTTTCTGCGGGAACTTCGT GCCAATGTGGAAGAAGAACGGGCAGCCCGCCTCCGCACGGCTAGCATCCCACTGGAGGCAGTGCGGGCAG AGTGGGAGAGGACGTGTGGGCCCTACCACAAGCAGCGTCTGGCTGAGTACTATGGC
Notes:	The probe template was PCR amplified from IMAGE:1887595 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1887595 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001892 same experiment
	EMAGE:30076 same embryo
	EMAGE:29682 same embryo
	EMAGE:29676 same embryo
	EMAGE:31733 same embryo
	EMAGE:29712 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source