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EMAGE:31733

Urod ( MGI:98916)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
telencephalon ventricular layer
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 weak expression: see section 11 12
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13
hypothalamus ventricular layer
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 11 12
liver
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 07 08
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 13 14 weak expression: see section 05 06 07 08 11 12
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 05 13 14 15
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 11 12
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T654
Entity Detected:Urod, ( MGI:98916)
Sequence:sense strand is shown

>T654
CTCGAGNCTGTTGGCCTACTGGAGTTACTGTTTACCATGGAGGCGAACGGGTTCGGACTCCAGAATTTCC
CGGAGCTGAAGAATGACACGTTCCTGAGAGCAGCCTGGGGAGAGGAAACAGACTATACTCCCGTTTGGTG
CATGAGACAGGCAGGCCGCTACTTACCAGAGTTTAGGGAAACCAGGGCTGCCCAGGACTTCTTCAGCACC
TGCCGATCTCCCGAGGCTTGCTGTGAACTGACTCTACAGCCACTACGAAGGTTTCCTCTGGATGCTGCCA
TAATTTTCTCTGACATCCTTGTTGTACCCCAGGCATTGGGCATGGAGGTGACCATGGTACCTGGCAAAGG
ACCCAGCTTTCCAGAGCCATTAAGAGAAGAGCGGGACTTAGAGCGTCTACGGGATCCAGCAGCAGCGGCT
TCAGAGTTAGGCTATGTGTTCCAAGCCATCACCCTTACTCGACAACGGCTGGCCGGACGTGTGCCACTAA
TTGGCTTTGCTGGTGCTCCGTGGACCCTAATGACATACATGGTTGAAGGCGGCAGTTCAAGCACCATGGC
Notes:The probe template was PCR amplified from IMAGE:1887550 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1887550 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009238 same experiment
 EMAGE:30076 same embryo
 EMAGE:29682 same embryo
 EMAGE:29676 same embryo
 EMAGE:30972 same embryo
 EMAGE:29712 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS