EMAGE mouse gene expression spatial database

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EMAGE:31040

U46068 ( MGI:2137431)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001886_01	euxassay_001886_02	euxassay_001886_03	euxassay_001886_04	euxassay_001886_05

euxassay_001886_06	euxassay_001886_07	euxassay_001886_08	euxassay_001886_09	euxassay_001886_10

euxassay_001886_11	euxassay_001886_12	euxassay_001886_13	euxassay_001886_14	euxassay_001886_15

euxassay_001886_16	euxassay_001886_17	euxassay_001886_18	euxassay_001886_19	euxassay_001886_20

euxassay_001886_21	euxassay_001886_22	euxassay_001886_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

stomach

strong

regional

strong expression: see section 07 08 09 weak expression: see section 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3497
Entity Detected:	U46068, ( MGI:2137431)
Sequence:	sense strand is shown >T3497 TGGCCTCGAGCCAGATTCGGACGAGGCCACTGGTTCACAACTCTGCGACTTCTCTGATGGAGACCACCCC AGACAGGGCCCCCTTCAGCCTGACCGTGAGGCAGGACCTGGTGAATGCCATTGTGACCACCCTGGTCCCC AAGGAGGAGCTTGTAATCCTGCTCAGATTCGTGATTCCTGATGTGGCCCGCCAGTTACAGATGGACATCA AGGAAATCAATGCAGAGGCAGCCAACAAGCTGGGGCCCACCCAGATGTTGAAGATCTTCACCCACAGCAC CCCCCACATTGTGCTGAACGAGGGCAGTGCCAGGGCAGCCCAAAGTGTCGTGCTGGAAGTGTTCCCTACC AACACTGATGTCCGGCCCTTCTTCTCTCTCGGCATTGAGGCCAGTTATGAAGCTCAGTTCTTCACAGAAG ACAACCGGCTTATGCTCAACTTCAATAACGTCAGTATTGAGCGCATCAAGCTGATGATCTCAGATATCAA ACTATTCGATCCTGAAGTCCTGAAGGACACCCTGACCAAGATCCTTGAATACACACTGCTGCC
Notes:	The probe template was PCR amplified from IMAGE:335127 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:335127 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001886 same experiment
	EMAGE:29824 same embryo
	EMAGE:29823 same embryo
	EMAGE:29536 same embryo
	EMAGE:31753 same embryo
	EMAGE:29701 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source