EMAGE mouse gene expression spatial database

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EMAGE:31056

Wfs1 ( MGI:1328355)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010102_01	euxassay_010102_02	euxassay_010102_03	euxassay_010102_04	euxassay_010102_05

euxassay_010102_06	euxassay_010102_07	euxassay_010102_08	euxassay_010102_09	euxassay_010102_10

euxassay_010102_11	euxassay_010102_12	euxassay_010102_13	euxassay_010102_14	euxassay_010102_15

euxassay_010102_16	euxassay_010102_17	euxassay_010102_18	euxassay_010102_19	euxassay_010102_20

euxassay_010102_21	euxassay_010102_22	euxassay_010102_23	euxassay_010102_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

spotted

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

vertebral axis musculature

moderate

spotted

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

maxilla

strong

spotted

strong expression: see section 06 07 08 09 10 17 18 21 moderate expression: see section 11 12 14 15 16 19 20

mandible

strong

spotted

strong expression: see section 05 06 07 08 09 10 17 18 21 22 moderate expression: see section 03 04 11 12 13 14 15 16 19 20

stomach

moderate

spotted

moderate expression: see section 02 03 04 05 06 07 08 09 10

lung

strong

spotted

strong expression: see section 05 06 07 08 09 10 14 15 16 17 18 19 21 22 moderate expression: see section 01 02 03 04 11 12 13 20 23

orbito-sphenoid

strong

regional

strong expression: see section 04 05 23 24 moderate expression: see section 01 02 03 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T32093
Entity Detected:	Wfs1, ( MGI:1328355)
Sequence:	sense strand is shown >T32093 TACGCCAAGGGCATCATTCCCACCAACCTGTTCCTGCAGGATGAGGATGAAGATGAGGACGAGCTGGCAG GGAAGAGCCCCGAGGACCTGCCACTACGCCAGAAGGTGGTGAAGTACCCTTTACACGCCATCATGGAGAT CAAAGAGTACCTGATTGACGTAGCCTCCAAGGCCGGCATGCACTGGCTCTCCACCATTGTACCCACCCAT CACATCAACGCCCTCATCTTCTTCTTCATCATCAGCAACCTAACCATCGACTTCTTCGCCTTCTTCATCC CCCTGGTGGTCTTCTATCTGTCCTTTGTGTCCATGGTCATCTGCACGCTCAAGGTGTTCCAGGACAGCAA GGCCTGGGAGAACTTCCGTACTCTCACCGACCTGCTGCTGCGCTTCGAGCCCAACCTAGACGTGGAGCAG GCCGAAGTGAACTTCGGCTGGAACCACCTGGAGCCCTACATCCACTTCCTACTGTCAGTCGTCTTTGTCA TCTTCTCCTTCCCGCTGGCCAGCAAGGACTGCATCCCCTGCTCGGAGCTGGCCGTCATCTCCACCTTCTT CACGGCGACCAGCTACATGAGCCTGAGCAGCTCTGCTGAGCCCTATACCAGGCGTGCCCTGGTCACCGAG GTGGCTGCCGGCTTGCTGTCCCTTCTGCCCACCGTGCCTGTGGACTGGCGCTTCCTGAAAGTACTCGGCC AGACTTTCTTCACTGTGCCCGTTGGCCACTTCATCATCCTCAACGTCAGCCTCCCCTGCCTGCTCTATGT CTATCTCTTTTACCTCTTCTTCCGCATGGCCCAGCTGAGGAACTTCAAGGGCACTTATTGCTACCTGGTG CCCTACCTGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6492038), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58117. Forward Primer - name:058117_F_IRAV90_h09_Wfs1, sequence:TACGCCAAGGGCATCATT; Reverse Primer - name:058117_R_SP6_IRAV90_h09_Wfs1, sequence:ACACCAGGTAGGGCACC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010102 same experiment
	EMAGE:29392 same embryo
	EMAGE:29370 same embryo
	EMAGE:31163 same embryo
	EMAGE:31177 same embryo
	EMAGE:29402 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source