EMAGE mouse gene expression spatial database

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EMAGE:31087

Rap1gds1 ( MGI:2385189)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003801_01	euxassay_003801_02	euxassay_003801_03	euxassay_003801_04	euxassay_003801_05

euxassay_003801_06	euxassay_003801_07	euxassay_003801_08	euxassay_003801_09	euxassay_003801_10

euxassay_003801_11	euxassay_003801_12	euxassay_003801_13	euxassay_003801_14	euxassay_003801_15

euxassay_003801_16	euxassay_003801_17	euxassay_003801_18	euxassay_003801_19	euxassay_003801_20

euxassay_003801_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 13 14 15 16 moderate expression: see section 12

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 13 14 weak expression: see section 15

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 14 15 16 17 18 19 20

ventral grey horn

strong

regional

strong expression: see section 08 09 11 12 13

glans of male genital tubercle

strong

regional

strong expression: see section 09 moderate expression: see section 12

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 15 16 17

pons mantle layer

strong

regional

strong expression: see section 06 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7041
Entity Detected:	Rap1gds1, ( MGI:2385189)
Sequence:	sense strand is shown >T7041 GCCCTGCCGGTCCTCACTCTGTGGGAGGAACATGGATAACCTCAGTGACACTTTAAAGAAGCTGAAGATA ACAGCTGCTGACAGGACAGAGGGCAGTCTGGAAGGATGCTTGGATTGTCTGCTTCAAGCCTTGGCTCAGA ATAATGCAGAAACAAGTGAAAAAATACAAGGAAGTGGAATTCTTCAGTTGTTTGCAAATCTGTTGACGCC ACAGGCCTCCTGCACAGCCAAAGTAGCCAACATCATAGCAGAAGTAGCCAAAAATGAATTTATGCGAATC CCATGTGTAGATGCTGGATTGATTTCACCACTGGTACAGCTGTTAAATAGCAAAGACCAGGAAGTGCTGC TTCAAACGGGCAGGGCATTAGGAAACATATGCTACGATAGCCATTCCCTTCAGGCCCAGCTTATTAGCAT GGGTGTTATCCCGACCTTAGTGAAGCTACTGGGCATTCACTGCCACAATGCAGCACTCACCGAGATGTGT CTCGTTGCGTTCGGGAATTTAGCAGAACTTGAGTCAAGTAAAGAACAGTTTGCTAGCACAAACATCGCTG AAGAGCTGGTAAAACTGTTCAAGAAACAGATAGAACACGATAAAAGAGAGATGATTTTTGAAGTCCTAGC GCCGTTGGCAGAGCATGATGCTATTAAGCTGCAGCTGGTTGAAGCAGGTTTGGTAGAGTGTCTCCTAGAG ATCGTCCAACAGAAGGTGGATAGCAACAAAGAGGACGACGTTGCCGAGCTTAAAACTGCTTCTGACCTCA TGGTTTTATTACTGCTTGGAGATGAGTCCATGCAGAAACTTTTTGAAGGAGGAAAAGGCAGTGTGTTCCA AAGGGTGCTCTCCTGGATTCCATCGAATAACCACCAGCTGCAGCTGGCTGGGGCATTAGCCATTGCAAAT TTTGCCAGGAACGATGGAAATTGTATTCATATGGTAGACAATGGGATTGTAGAAAAACTCATGGATTTGC TGGACAGACACGTAGAAGACGGGAACGTGACGGTGCAGCACGCCGCACTAAGTGCCCTCAGGAATCTAGC CATCCCAGTTGTAAATAAAGCAAAGATGTTATCAGCTGGTGTCACGGAGACGGTTTTGAAATTCCTTAAA TCTGAAATGCCCCCAGTTCAGTTCAAACTCCTGGGAACGCTAAGAATGTTAATAGATGCACAAGCAGAAG CTGCTGAGCAACTGGGAAAGAATGCTAAACTGGTGGAGCGTTTGGTTGAATGGTGTGAAGCCAAAGATCA TGCCGGCGTGATGGGCGAATCAAACAGACTGCTCTCTGCCCTCATCCGACACAGTAAATCAAAAGATGTA ATTAAAACCATTGTACAGAGTGGTGGCATCAAGCACCTGGTTACCATGGCAACTAGTGAACATGTAATCA TGCAGAATGAAGCTCTTGTCGCCCTGGCACTGATAGCAGCCTTGGAACTGGGTCCCGCTGAGAAGGATCT CGCAAGTGCTCAGCTTGTTCAGATCTTACACCGACTGCTAGCAGATGAGAGAAGTGCCCCGGAGATCAAG TACAATTCCATGGTCCTGATCTGCGCTCTCATGGGATCCGAATCTCTATACAAGGAAGTACAGGATTTGG CCTTTCTAGATGTTGTCTCCAAACTTCGAAGTCATGAGAACAAAAGTGTTGCCCAACAGGCCTCGCTTAC AGAGCAGAGACTTACAGTGGAAAGCTGAGAACTGGCCCTGCTGGCACCAGGCTCATCCCAGCTCCGCCCA CTCCACTGTTCTCCATCCAGCTGCTTCTTCAACTCCATTCTCTACTACATCACTTGTGCATGCGTGTAAT GTTCCAGTACCAATGGAAGAACTGCTATAGGTACCTGCCTAATAAGATTTCTAAACCCATAGTTAATGTG AACATGACTGTCAGAACAAGTCTCCACCCATAACTGTTAAATCCTATACTCCTGATGCTTGAGCTACATT AAGTAGAATGTGCATGTTGTAGTCCTATGATGATGTAAACTTGGTACTCCATGCTGACTTGCTCCTCACA TGCAGTAAGCTACATAATAATGTACTGGTAAACTAGAAACAAAGAATGCAGCAGGACCTGTCTAGCTTAT TTAAAGATGAGACTGAATCGTCAAGGTAGCACCACGTATTCTGGTTTTGTTTTGTTTTGGTGCTGGGGAA GCACAGCGATCAAAAAGCCTGTATGGCTGCTCACTCATTAATCTTCTGCCAACGTCAGGCCTGTTCCACC TAAACTTGAATAGGTCCAATATTCCTTCCTTCCCTACGGTCTCTCCTTTTTCTGAACTATATAGGCAGTT TGCCAAGTAACACAGCAAACCTTCCTGAGGCTCCTGATTCCATCTTTAATCGACAGTGGTACAGGGGCTC AGTGCTGAAACGTGGATTTTAATGTGAGCGGAGCGTAGGAAGGAGTTAAAGAAACCCTGGCATACTGAAC CACTTTGGATCTTGCCTGTACTTTAGATCCGATTTGTGTGTGGTTACATCTAGAACTTCCATCAGCTAGC CAGCATTGTCAACATGTCTCTAAAGCGTCCTTGGTGTTTGTTCGGTGAAGGAAACTGAGGCGGCTTGTTT GGTGGAGAATGGACTTCCCCCCCCCGCCCCGTTTTCATCTTTTAGGGTGAAAGCAGGGAGGTTCATCTTT CATGCTCCCTTTCAATGTGCTAATTCTAATTGGACATAAGAGGAAACCTGAAGTAAGCTTGTCTAAAGAG AGGAAGGCCGGTTTTAAAAGCCATAGTAGCCAGTCCAGTACATTCTGCAGATGGCCTCATAGCAGTCATT GTTAGAGTTCTTCACCTCCACTTTCCCACTGAGAACCACAATAATCTCCCAGGCTGCAAGCCAGGACTCG CCGCAAAGACTCTTCTTGGCATAATGTAGTGTGCTCGTCACTGCCACTAACCACCACCAAGTGAGTTCTT AGAGCTTTGACACACAGTCTTTATAAATTAATCGGGATTCCCTGTGACCCGCCTGCCTCCTCACACCCAA GGTTGAGTCCTGCTGATGTCCGAGGGAGGCTGAGCTCCGTTACAGGAGTGGAGGGCAGTGAGGGGCTGCT GAGAGGGACCAGCTCCTCCCTCCCCAGCTCCTCCCTCCCCCAACAGGAGATACCTGTATCTCTGCGCTCT CTAAGTCGGACTTTGCTTTTCACACTTTTAAATTCAAGAAAGATTTACTTCATGTAAGTAGAAACCTGAC TGACAGTTCCCTCTGATGTCCAAATAAGGGAACTGTGGTGAGCGCCTGTGCTTTATTGTCTTTCTCCTGG GCTGGATAAGTGGACTAATGTTGCATAATACTGTCCATATTAATAACTTAATAAATAAATAGTTTATGAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4008751 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4008751 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003801 same experiment
	EMAGE:31738 same embryo
	EMAGE:31171 same embryo
	EMAGE:31217 same embryo
	EMAGE:31214 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source