EMAGE mouse gene expression spatial database

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EMAGE:31217

1810003N24Rik ( MGI:1913499)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003781_01	euxassay_003781_02	euxassay_003781_03	euxassay_003781_04	euxassay_003781_05

euxassay_003781_06	euxassay_003781_07	euxassay_003781_08	euxassay_003781_09	euxassay_003781_10

euxassay_003781_11	euxassay_003781_12	euxassay_003781_13	euxassay_003781_14	euxassay_003781_15

euxassay_003781_16	euxassay_003781_17	euxassay_003781_18	euxassay_003781_19	euxassay_003781_20

euxassay_003781_21	euxassay_003781_22	euxassay_003781_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23

stomach

moderate

regional

moderate expression: see section 02 03 04 05 weak expression: see section 06 07 08 09

rectum

weak

regional

weak expression: see section 13

urethra of male

moderate

regional

moderate expression: see section 12 13

left lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12

axial musculature

moderate

regional

moderate expression: see section 04 05 06 20 21 22

midgut

weak

regional

weak expression: see section 12 13 14 15 16 17 18 19 20

bladder

moderate

regional

moderate expression: see section 12 13 14

esophagus

moderate

regional

moderate expression: see section 13

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 12 13 17 weak expression: see section 09 10 16

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 19 20 weak expression: see section 18

thymus primordium

weak

homogeneous

weak expression: see section 11 12 13 14 15

hindgut

weak

regional

weak expression: see section 13 14 15 16

orbito-sphenoid

moderate

regional

moderate expression: see section 01 04 05 06 21 22 23 weak expression: see section 02 03 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7038
Entity Detected:	1810003N24Rik, ( MGI:1913499)
Sequence:	sense strand is shown >T7038 CGGACGCGTGGGCAACCACGCACGTGTTCCTCTAGTCGTACTTGCCGGGATTGATCTTGGCCGAGATGGA GACGCAAAGCACCGGGACGGAGGACGGCTTTACTCCCGTCACGCACAGAGGGGGCCGCCGGGCGAAGAAA CGACAGGCTGAGCAGTCGTCAGCGGCGGGACAGGATGGAGAAGCCGGACGCATGGACACGGAGGAGGCGC GGCCGGCGAAGAGGCCGGTCTTCCCGCCGCTCTCCGGGGATCAGCTCCTGACTGGGAAAGAAGAAACGAG GAAAATTCCCGTCCCAGGTAACAGATACACACCGTTGAAAGAAAACTGGATGAAGATATTTACTCCGATT GTAGAACATTTGGGACTTCAGATACGCTTTAACCTGAAATCAAGGAATGTAGAAATCAGGACTTGTAAAG ACACCAAGGATGTCAGTGCCCTGACAAAAGCGGCCGACTTTGTGAAAGCCTTTGTTCTTGGCTTTCAGGT GGAGGATGCACTTGCCCTTATCCGACTAGATGACCTCTTCCTAGAGTCTTTTGAAATAACTGATGTTAAG CCCCTAAAAGGAGACCACCTGTCAAGGGCAATAGGAAGAATTGCTGGCAAAGGAGGAAAAACCAAATTTA CCATAGAGAACGTAACACGGACACGGATTGTCCTGGCAGATGTGCACGTTCACATTCTTGGCTCTTTCCA AAATATCAAGATGGCAAGAACAGCTATTTGCAACCTCATCCTAGGAAATCCTCCATCAAAGGTGTATGGC AATATCCGAGCTGTGGCCAGCAGATCAGCAGATCGGTTCTGATTTTCCATGAACCCTTGAGATGTTCACA TAAAACCATGGGAAGAATTTCCCAGCCACTTAGAGCTCAGGTTTCTTCTCAGCTCTCAGCCAGAGCAGAA GCATAAAAGGAAGGTTTAACAGGACTTGTACTCAGCTGCTTCTAGGATAATTTAAATATGATATAATACT GAAAAGTACAGCAGTTTATACTTAAAGTGTAAGTAAGCCACACGGGCAGATGCACAGGTTACAACCCTAT CAATTAGAGGTAACTGCTTGCTTACAACTCTTTGGTTACAGATTTACAGCTAAACTTATAAACTTTATAT AAACTCTGAAATTTTTAATTAAAAATGAATCACAATTCTTGGTGCCAGGCACTGTGACTATTTCAGGGTT GAGTCATGGCAGAAACTTGCACTATTGAACTATATGTGCTCATCTTTGTACTTCCTGTACAATTTATAAA ATGAAAGTCCTGCATCACGTGGCTAATAAATGTTATTTTTCAAATAATTAAAGATGTTAAAATATTTCTC ATGTGCAGTAAAAGTAAAAATGTAAAAGAATTCTCCCAAGAAAACTGTTCTCCAAACAGCATATTCTACT AGTGTCTGTGTGTATGAATTTGATTATTTCTTAAATTGTTTTAGAACACATTTCATTAAACATGTAACTT AGATACTACATTCTCATGTTTCTCAAAAGCCTTAACCAAAATATTGTAATGATTGGAGATCAGTAAATTC AAGCAATAACTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4007940 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4007940 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003781 same experiment
	EMAGE:31738 same embryo
	EMAGE:31171 same embryo
	EMAGE:31087 same embryo
	EMAGE:31214 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source