EMAGE mouse gene expression spatial database

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EMAGE:31260

Cab39 ( MGI:107438)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003762_01	euxassay_003762_02	euxassay_003762_03	euxassay_003762_04	euxassay_003762_05

euxassay_003762_06	euxassay_003762_07	euxassay_003762_08	euxassay_003762_09	euxassay_003762_10

euxassay_003762_11	euxassay_003762_12	euxassay_003762_13	euxassay_003762_14	euxassay_003762_15

euxassay_003762_16	euxassay_003762_17	euxassay_003762_18	euxassay_003762_19	euxassay_003762_20

euxassay_003762_21	euxassay_003762_22	euxassay_003762_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 13 14 15

facial vii ganglion

weak

regional

weak expression: see section 03 04 19

not examined

homogeneous

not examined expression: see section 01 02 03 04 22 23

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 22 23

vagus x ganglion

weak

regional

weak expression: see section 06 07 15

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 not examined expression: see section 15

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 17

clavicle

moderate

regional

moderate expression: see section 07 08 14 15

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6998
Entity Detected:	Cab39, ( MGI:107438)
Sequence:	sense strand is shown >T6998 GAAGCAGAGAGGGAGCTCCCTGGCCCGGCCCAGCCCGGTCAAGCAGGTAGAGTGCCGTGGAAGCCTCTGG CGCTGCCGTCATGCCGTTCCCATTTGGCAAGTCTCACAAGTCTCCGGCAGATATTGTGAAGAACTTGAAG GAGAGCATGGCTGTTTTGGAAAAGCAGGACATTTCTGACAAAAAGGCAGAAAAGGCTACAGAAGAAGTTT CCAAAAATTTGGTCGCCATGAAAGAAATTCTGTACGGCACCAATGAGAAGGAGCCTCAGACAGAGGCGGT AGCTCAGCTGGCTCAGGAGCTGTACAATAGCGGGCTCCTCGGCACCCTGGTAGCTGACTTACAGCTCATT GACTTTGAGGGCAAAAAAGACGTGGCTCAAATTTTCAACAATATTCTCAGAAGACAAATTGGTACAAGAA CTCCTACTGTTGAATACATCTGCACCCAACAGAATATTTTGTTCATGTTATTGAAAGGGTATGAATCTCC AGAAATAGCTCTTAATTGTGGGATAATGTTAAGAGAATGCATCAGACATGAACCACTTGCAAAAATCATT TTGTGGTCAGAACAGTTTTATGACTTCTTCAGATATGTTGAAATGTCAACATTTGACATAGCTTCAGATG CATTTGCTACATTCAAGGATTTACTTACAAGACATAAATTGCTCAGTGCAGAATTTTTGGAACAACATTA TGATAGATTTTTCAGTGAATATGAAAAGCTACTTCATTCAGAAAATTATGTGACAAAGAGACAGTCACTG AAGCTTCTGGGTGAGCTGCTGTTGGACAGACACAACTTCACAATTATGACAAAGTACATCAGCAAGCCCG AGAACCTCAAGCTAATGATGAACCTCCTCCGAGACAAGAGCCGCAACATCCAGTTCGAGGCCTTCCACGT GTTCAAGGTGTTTGTGGCCAACCCCAACAAGACGCAGCCCATCCTAGACATCCTCCTCAAGAACCAGACC AAGCTCATCGAGTTCCTCAGCAAGTTTCAGAACGACAGGACCGAGGACGAGCAGTTCAACGACGAGAAGA CCTACTTAGTCAAGCAGATCAGGGATTTGAAGAGAGCCGCCCAGCAGGAAGCCTAGTGTCTACTCAGCCC CGGTCCCATCCAAGCTCAGCACTGGCTGTCAGCTCCTCAGCATCAGGCACTCTCATCGGTTCATGGGGAG CACTACTGCTAATCTGCTGTTAAGTGAACGGTTTTTCATTTTACCTTTTTGTTTTTCAGTCCAGGTTGGA GAACGTAGCTGCTGCTGCTTGCACATTAGAGCGCACGTGGTCTTTCTTTCCCTTGATCTCTGTGTCATTT CAGAACTCACAGACTCCATTTGCTACAGGAGTTCTGAACATGGCTGGGTTCATGAAAGCAAATGAGAGTG AGAGTGGTCTAGGGAAAAGACGGTGATACATCGCTGACACCTGCCCGTCCATCGGCACTGCTCTCTGCAG GTCTGTGCAGTATGGCACGGGCATACCCACCCATCTCTGCCAAAGGAAGATGGCGCATACAGGGATTGTT GGAGAACTGTATCCAGTGTGAGCTTTGGGGAGGATAAAGTTGGTGTGCAAATAAATTGATACTGAAGCTT AGCAACTTGTCAAGTGTGGAGCTTCATAAGGTCATAAGAAAGTGTATATATGCTTTTTCACAGCTTTCTA GAACTTTGTGACATTTGATTTTCTTGAAACTTGTAAACCTGGATAGGTTGAAGGGTGTTTTAATTTTACT TTTGAAGGTGTTTTAAAACAGTAGAGCTAGCAGCAACACCTTATGTTTCATTTCAACACTGCTTACAGGT TTCTTTTGTATATAATTCTTAGAATGCTCATTTCTTTTAAATCGTTTAATTTGTACAGCAGAGGAATGTT ATTGTAGTAGTATGTAACTATTACCTGAGTTTTGCAACAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3990872 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3990872 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003762 same experiment
	EMAGE:31192 same embryo
	EMAGE:31881 same embryo
	EMAGE:29424 same embryo
	EMAGE:31258 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source