EMAGE mouse gene expression spatial database

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EMAGE:31881

Slc35f2 ( MGI:1919272)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003909_01	euxassay_003909_02	euxassay_003909_03	euxassay_003909_04	euxassay_003909_05

euxassay_003909_06	euxassay_003909_07	euxassay_003909_08	euxassay_003909_09	euxassay_003909_10

euxassay_003909_11	euxassay_003909_12	euxassay_003909_13	euxassay_003909_14	euxassay_003909_15

euxassay_003909_16	euxassay_003909_17	euxassay_003909_18	euxassay_003909_19	euxassay_003909_20

euxassay_003909_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

genital tubercle of female

moderate

regional

moderate expression: see section 11

palatal shelf

moderate

regional

moderate expression: see section 10 11 12

metanephros

moderate

regional

moderate expression: see section 06 07 08 12 13 14 15 16

rectum

moderate

regional

moderate expression: see section 10 11

nose

moderate

regional

moderate expression: see section 09 10 16

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

medulla oblongata lateral wall

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13

pancreas

weak

regional

weak expression: see section 07 08 09 10 11 12 13

olfactory lobe

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

lower jaw molar

strong

regional

strong expression: see section 15 moderate expression: see section 07 08 09 13 14 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 12 13 14 15

spinal cord lateral wall

moderate

regional

moderate expression: see section 07 08 09 10

upper jaw molar

strong

regional

strong expression: see section 15 moderate expression: see section 07 08 09 13 14 16 17

thymus primordium

weak

regional

weak expression: see section 09

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10

naris

moderate

regional

moderate expression: see section 10

telencephalon mantle layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 17 18 19 20 21

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 12

not examined

regional

not examined expression: see section 10 11

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6996
Entity Detected:	Slc35f2, ( MGI:1919272)
Sequence:	sense strand is shown >T6996 GCTTCCCAAAGGTGCCAGGTGGCGATCTGCAAAAGCATTGTCTGGCCCTTAGGGCCTGACAGTTAATGAT TTGTCGCTCGAGCGGGGCGGAGGAGTATCGGGACGCGGGCGAGCATACTAGAGAGCGCAGCAGGAGAGCA AGGCAGGGCGCGAGCCGAGTCCGCGCCGTACTCACAGGCACCGCCTCCTGTCTTCCGGGCCCGAGCACTT CTCGGGGTTCCGGGCACGCGAGGCTCGAAGAGACCCCATGGAGGCTGAGTCGCCGGCGAGTGCGGGGGCC AGCGAGCCCCGGGCGCTGGGTACGGTGGACAGTATCGGCAGCCAGCTGAGCAGGATCCGACGCAAACTCT TCACCTGGGATATTTTGAAGACCATAGCTCTGGGCCAGATGCTGTCTTTGTGTATTTGCGGCACGGCCAT CACCAGCCAGTATTTGGCAGAAAAGTACAGAGTGAACACGCCCATGCTGCAGAGTTTCATCAACTACTGC TTGCTCTTCCTGGTTTACACGGTGATGCTGGCGTTTCAGTCAGGCAGTGATAACCTCCTGGAGATTTTGA GAAGAAAATGGTGGAAGTATACTCTCTTGGGGCTTGCCGATGTGGAAGCCAATTATCTGATTGTCAGAGC ATACCAGTACACGACTCTGACCAGTGTCCAGCTCTTGGATTGCTTTGGGATTCCTGTGTTGATGGCTCTC TCCTGGTTTATTCTCCGGGCAAGATACAAAGTGATCCACTTCATTGCTGTGTTTGTCTGTCTGCTGGGTG TAGGGACCATGGTTGGTGCAGACATATTAGCTGGGCGAGAAGATAATTCAGGGAGTGATGTTCTTATTGG TGACATCTTAGTCCTGCTTGGGGCTTCCCTTTATGCTGTGTCTAACGTGTGTGAAGAATACATCGTGAAG AAGCTGAGCAGACAGGAATTTTTAGGAATGGTGGGACTATTTGGAACAATCATCAGCGGCATACAGCTAT TGATTGTGGAATATAAAGACATTGCCCGGATTCAGTGGGACTGGAAAATTGCCCTGTTATTTGTGGCATT TGCCCTCTGTATGTTTTGCCTGTACAGCTTCATGCCATTGGTGATTAAAGTCACGAGTGCCACTTCAGTC AACCTGGGCATCCTGACAGCTGACCTCTACAGCCTTTTCTTTGGGCTTTTTTTATTCGAGTATAAGTTCT CAGGACTCTACATCCTGTCGTTCACAGTCATCATGGTTGGCTTCATCTTGTACTGTTCCACCCCAACGCG CACGGTAGAGCCCCCAGAAAGCAGCGTGCCCCCTGTTACCAGCATCGGGATCGACAATTTGGGACTGAAG CTTGAGGAGAGCGGTCTCCCGGAGACCCACTCTGCAGTCTTGTAGCTGGACAAGCCACAGACTCTGGCAT GTCACCAGGAGAGAGCAGAACCTGCCACTGCCAAGGGCGCATTTGAGGCGACACCAGCCTCAGAGGCGAA ACCCACTGCATTGGATTACATCCGTGGTTAGATTGTAGAAAGGAGCACTGACCAGTGGTTCCAAACTAGA AATACTAAAATAGAGCAGAGAGCCCAAGGGTAGTGAGTGTTTGCGTGTGCTGAGTACCAGTACCTGTCAG CGTCAGGTTTCAGAGACACATGTAAGAAGAGAAGGGGACCTGCTTCAGGGCTGTTTAGGTAGTTAGGAGT TGGGACAGTCTGGGATGTTGGTGCAATAAGATTTCTCTGTTGGAAGCTTCACGTTTTCATCCTGCTGGGG CAGTCACTGTCCATGGACCTTGTGGAATTTAGACACCCATGACCGCTGGTCCTTGGTGGCAGGTGGTGGG AAAAATGGGCGGGGCAGCACTTGAGAACTCCCAAGATAAACTGTGACGACTTTGAAAGCAGCTAATGAGT ACAGGAATCCAGGAACCCTCTCCCAGTCATATCCCTCGATGTTTTATAACATGTCTCTCCCCTTTGTCCC TCTTGCTTGTCCCTAGGCACGTTTGCTCCTGTGTTCTGAGTTCTGGAGCAAACTGCTCTCAAAGCCTGTT ATATTGGGCATCCCTCCCATTCAGCGCCACACTATACGCTGAGAGCAAATACGATCAAGTTACCCTTGGG TAATGTCCCGGTGCCCGGGAAGAGTCATCCCAATTGGCCTCACCTCACCAAGCCACAGTTTAGAAAATGA ACCTGCTGCAGCGTCTCATAGCAAGGCAAGAGCTTGAGAACTTGGTTGCTGTCTCTGGTTGGAAAAAATG CATTATTGTTAAAAGGAAAACGCACTGCTAATCATTCCCCACTGAAGCGAGGAACACTGACCAATGGTTC CACGCTAGGAATCCTACAGTAGGTTGGTGCTGGGGTTCTGGAGTCCTGACTGTAAGAGGAGAGTGTCTTC CTTCTTCCCACAGGTTTACAATGCTTGCAGGTTTGTGACAATTCCTCAGCATGGTGTCAACATGTAGCCT TAGCATAACATCAGCCACCAGAGTGCCGCCTTCAGAAAACTTTATGTAAATTGTTTTTGTATAAGTATAA TAAAGAAATTTGGGGTAAGTGTTCGTATTATTAAAAGACTTTAAACTCATGAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3991126 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3991126 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003909 same experiment
	EMAGE:31260 same embryo
	EMAGE:31192 same embryo
	EMAGE:29424 same embryo
	EMAGE:31258 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source