EMAGE mouse gene expression spatial database

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EMAGE:31267

Amd1 S-adenosylmethionine decarboxylase 1 ( MGI:88004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002401_01	euxassay_002401_02	euxassay_002401_03	euxassay_002401_04	euxassay_002401_05

euxassay_002401_06	euxassay_002401_07	euxassay_002401_09	euxassay_002401_10	euxassay_002401_11

euxassay_002401_12	euxassay_002401_13	euxassay_002401_14	euxassay_002401_15	euxassay_002401_16

euxassay_002401_17	euxassay_002401_18	euxassay_002401_19	euxassay_002401_20	euxassay_002401_21

euxassay_002401_22	euxassay_002401_23	euxassay_002401_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 21 22 23

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3052
Entity Detected:	Amd1, S-adenosylmethionine decarboxylase 1 ( MGI:88004)
Sequence:	sense strand is shown >T3052 TGGCCTCGAGGCCAGATTCGTCGACTCGGACCAGTTCTACATGAAAGATGGTGTTACTGCAAAGGATGTC ACTCGTGAGAGTGGAATTCGTGACCTGATACCAGGTTCTGTCATTGATGCCACACTGTTCAATCCTTGTG GCTACTCGATGAATGGAATGAAATCGGATGGAACATATTGGACTATTCACATCACTCCAGAACCAGAATT TTCTTATGTTAGCTTTGAAACAAACCTAAGTCAGACCTCCTATGACGACCTGATCAGGAAAGTTGTGGAA GTCTTCAAGCCAGGAAAATTTGTGACCACCTTGTTTGTTAATCAGAGTTCTAAATGTCGCACAGTGCTTT CTTCGCCCCAGAAGATTGACGGCTTTAAACGTCTTGATTGCCAGAGTGCTATGTTCAACGATTACAATTT TGTTTTTACCAGTTTTGCTAAGAAACAGCAACAACAGCAGAGTTGATTAGGAAAAATGAAGAAGAAAAAA CGCAAAAAGAGAAGACACACAGGAGGTGGTGGCTGCTTTCTAGATGCTGATCC
Notes:	The probe template was PCR amplified from IMAGE:1546344 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546344 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002401 same experiment
	EMAGE:31154 same embryo
	EMAGE:31274 same embryo
	EMAGE:29448 same embryo
	EMAGE:30301 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source