EMAGE mouse gene expression spatial database

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EMAGE:31415

Ltf ( MGI:96837)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006865_01	euxassay_006865_02	euxassay_006865_03	euxassay_006865_04	euxassay_006865_05

euxassay_006865_06	euxassay_006865_07	euxassay_006865_08	euxassay_006865_09	euxassay_006865_10

euxassay_006865_11	euxassay_006865_12	euxassay_006865_13	euxassay_006865_14	euxassay_006865_15

euxassay_006865_16	euxassay_006865_17	euxassay_006865_18	euxassay_006865_19	euxassay_006865_20

euxassay_006865_21	euxassay_006865_22	euxassay_006865_23	euxassay_006865_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

strong

single cell

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6647
Entity Detected:	Ltf, ( MGI:96837)
Sequence:	sense strand is shown >T6647 GCGAGCGGCCGTCGCGATCTAGAACCTCCCAAGACCACAGACATGAGGCTGCTCATCCCTTCCTTGATAT TTCTTGAGGCCCTTGGACTCTGTCTAGCTAAGGCAACAACTGTTCGATGGTGTGCTGTGTCAAATTCTGA GGAAGAAAAATGTTTAAGGTGGCAGAACGAGATGAGAAAAGTGGGTGGCCCGCCGCTCAGTTGTGTCAAG AAATCCTCCACCCGCCAGTGCATCCAGGCCATTGTGACAAACAGAGCTGATGCCATGACTCTTGATGGTG GCACTATGTTCGATGCAGGAAAGCCCCCCTACAAACTGCGACCTGTGGCAGCTGAAGTCTACGGGACCAA AGAGCAGCCCCGGACTCACTACTATGCGGTAGCAGTCGTGAAGAACAGCAGTAACTTTCATCTGAACCAA CTCCAAGGCCTGAGGTCCTGCCACACCGGCATTGGCAGGAGTGCGGGGTGGAAAATCCCTATAGGGACAC TTCGTCCATACCTGAATTGGAATGGGCCACCTGCATCCCTTGAGGAAGCGGTATCCAAGTTCTTCTCAAA GAGCTGTGTTCCCGGTGCCCAAAAGGATAGATTCCCCAACCTGTGTAGCTCGTGTGCAGGGACAGGAGCC AACAAATGTGCCTCTTCCCCCGAGGAGCCATACTCAGGTTATGCTGGAGCCTTGAGGTGTCTGAGAGACA ATGCTGGAGATGTGGCTTTTACCAGAGGAAGCACGGTATTTGAGGAGTTACCAAATAAAGCCGAAAGGGA CCAGTACAAGCTGCTCTGCCCAGACAACACCTGGAAGCCGGTGACAGAATACAAGGAGTGCCACCTGGCC CAAGTCCCTTCACATGCTGTCGTATCCCGAAGCACGAATGACAAAGAAGAGGCCATCTGGGAGCTTCTCC GCCAGTCACAGGAGAAGTTTGGAAAAAAACAAGCATCGGGATTCCAGCTCTTTGCCTCCCCCTCGGGACA GAAGGACCTGCTGTTCAAGGAGTCTGCCATTGGCTTTGTGAGGGTTCCCCAGAAGGTAGATGTAGGGCTC TACCTGACCTTCAGCTACACCACATCCATACAGAACCTGAATAAAAAGCAGCAGGATGTGATAGCCTCAA AGGCCCGGGTCACATGGTGTGCCGTGGGCAGTGAGGAGAAGCGCAAGTGTGATCAGTGGAACAGAGCAAG CAGAGGCAGGGTCACCTGCATCTCATTCCCCACCACGGAAGACTGCATTGTCGCAATCATGAAGGGAGAT GCTGATGCCATGAGCCTGGATGGAGGCTATATCTACACTGCGGGCAAGTGCGGTTTAGTTCCAGTCTTGG CAGAGAACCAGAAATCCTCCAAAAGCAATGGCTTGGATTGTGTGAACAGACCAGTGGAAGGGTACCTTGC TGTAGCAGCAGTTAGAAGAGAAGATGCTGGCTTCACCTGGAGCTCTTTGAGAGGCAAGAAGTCCTGCCAC ACTGCCGTGGACAGGACCGCAGGCTGGAACATCCCCATGGGCCTGCTTGCTAACCAGACCAGATCCTGCA AATTTAATGAGTTCTTTAGCCAAAGCTGTGCCCCTGGTGCTGACCCCAAATCCAATCTCTGTGCCCTGTG TATTGGTGATGAGAAGGGTGAGAACAAGTGTGCTCCCAACAGCAAAGAGAGATACCAAGGCTACACTGGG GCTTTAAGGTGTCTGGCTGAGAAGGCAGGAAATGTTGCATTTTTGAAGGACTCCACTGTCTTGCAGAATA CTGACGGGAAGAACACTGAAGAGTGGGCTAGGAACTTAAAGCTGAAGGACTTTGAGCTTTTGTGCCTTGA TGACACCCGGAAACCTGTGACTGAGGCTAAGAACTGCCACCTAGCCATAGCCCCAAACCATGCTGTAGTG TCTCGGACAGACAAGGTGGAAGTCCTTCAGCAGGTGCTGCTTGACCAACAGGTTCAGTTTGGGAGAAATG GACAGAGGTGTCCAGGAGAGTTTTGCCTGTTCCAGTCTAAAACCAAAAACCTTCTGTTCAATGACAACAC TGAGTGTCTGGCCAAGATCCCCGGCAAAACCACATCGGAGAAGTATCTGGGAAAGGAGTACGTCATAGCG ACCGAGCGCCTGAAGCAGTGCTCCAGCTCCCCACTCCTGGAAGCCTGCGCTTTTCTTACCCAGTGAAAAC ACTGAGCAAATAGCAGAACCTTCCCAGGAAGTCTCATCCCGGAGCCACGGTCCGGGGGCCTTCAGACCAT CTGGTCTCCTCACTCCCTGCTGTCACTTTAGGTAGAAATAAAATGAAGTAGTGTTGAAAAAAAAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600033 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600033 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006865 same experiment
	EMAGE:31947 same embryo
	EMAGE:29505 same embryo
	EMAGE:31944 same embryo
	EMAGE:31176 same embryo
	EMAGE:29523 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source