EMAGE mouse gene expression spatial database

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EMAGE:31947

Lat ( MGI:1342293)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006924_11	euxassay_006924_12	euxassay_006924_13	euxassay_006924_14	euxassay_006924_15

euxassay_006924_16	euxassay_006924_17	euxassay_006924_18	euxassay_006924_19	euxassay_006924_20

euxassay_006924_21	euxassay_006924_22	euxassay_006924_23	euxassay_006924_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15 16

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7356
Entity Detected:	Lat, ( MGI:1342293)
Sequence:	sense strand is shown >T7356 CCACGCGTCCGCGGACGCGTGGGCGGGGATCGTGCAGCTGGTAGCTGGGGCACGGGCCCCAGCTCTGGCT CTGGGGCGAGCACCTTTCCAGAGCCAACACTGCTCTCAACTCAGTCCAGCAAGAGAGGGGAGCCATCCAG CCCCGAAAGGATACGGCTGCCTACTGCCGGGCGGATCCCAGGCTGGAGCCCGCTTGGTCCCATACCCCTG CTGCCACTCTGTCTCGAGGGGCTGCAGTGCAGCAGGGCCTGTGGCAGGTGCTCTGCAGATGGAAGCAGAC GCCTTGAGCCCGGTGGGGCTGGGCCTCCTGCTGCTGCCCTTCTTGGTCACGCTCCTGGCTGCCCTGTGCG TGCGCTGCCGTGAGTTGCCAGTCTCCTATGACAGCACTTCCACAGAGAGTTTGTACCCAAGAAGCATCCT CATCAAGCCACCTCAAATAACCGTCCCCCGAACACCTGCTGTTTCCTACCCTCTAGTCACTTCCTTCCCA CCCCTGAGGCAGCCAGACCTGCTCCCCATCCCGAGATCCCCACAGCCCCTTGGGGGTTCCCATCGGATGC CATCTTCCCAGCAGAATTCAGATGATGCCAACAGTGTGGCAAGCTACGAGAACCAGGAGCCAGCCTGTAA GAATGTGGATGCAGATGAGGATGAAGACGACTATCCCAACGGCTACCTAGTGGTGCTGCCTGACAGTAGT CCTGCTGCCGTCCCTGTTGTCTCCTCTGCTCCTGTGCCTAGCAACCCTGACCTTGGAGACAGTGCCTTCT CTGTGGAGTCGTGTGAAGATTACGTGAATGTTCCTGAGAGTGAGGAGAGCGCAGAGGCGTCTCTGGATGG GAGCCGGGAGTATGTGAATGTGTCCCCAGAGCAGCAGCCAGTGACCAGGGCTGAGCTGGCCTCTGTGAAC TCCCAGGAGGTGGAAGACGAAGGAGAAGAGGAAGGGGTGGATGGAGAGGAAGCTCCCGACTATGAGAATC TACAGGAGCTTAACTGAAAGCCTACTGCAGCTGTCTGTCCTGAAACTGGACTTGCTGGGGTGTCGCTAAG AGGATCCCATTTGATCTCTGCCTTGCCACAGCCTGAGAATCTTCCCCTAACTTATTGTCACTTTGGGGTC CAGTCTGTGTCCCCAATATTCTGTACCTTCTGATAAAGCCTGAGAATGAATCTGGTTCCAGCCAGACCAT GTCATGGAATAAAGGCCATGTGACATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4191983 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4191983 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006924 same experiment
	EMAGE:29505 same embryo
	EMAGE:31944 same embryo
	EMAGE:31176 same embryo
	EMAGE:31415 same embryo
	EMAGE:29523 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source